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Mini protean tgx 4 to 15 gradient gels

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The Mini-PROTEAN TGX™ 4 to 15% gradient gels are a type of polyacrylamide gel used for protein electrophoresis. They provide a gradient of polyacrylamide concentration ranging from 4% to 15%, allowing for the separation and analysis of a wide range of protein molecular weights.

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Lab products found in correlation

2 mercaptoethanol, by Bio-Rad (2 mentions) Precision plus protein dual color, by Bio-Rad (2 mentions) Polyscreen pvdf hybridization transfer membranes, by PerkinElmer (2 mentions) Halt protease inhibitor, by Thermo Fisher Scientific (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Image lab software, by Bio-Rad (2 mentions) Laemmli sample buffer, by Bio-Rad (2 mentions) Chemidoc imaging system, by Bio-Rad (2 mentions) Du 640, by Beckman Coulter (2 mentions)

2 protocols using mini protean tgx 4 to 15 gradient gels

1

Western Blot Analysis of Activated T Cells

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Purified stimulated Tconv were lysed in radioimmunoprecipitation assay (RIPA) buffer with Halt protease inhibitor (Thermo Fisher Scientific). Protein concentration was determined by photometry (DU640, Beckman-Coulter). Subsequently, samples were mixed with Laemmli sample buffer containing 2-mercaptoethanol (Bio-Rad Laboratories, Hercules, CA) and boiled for 5 min. The protein samples were loaded onto Mini-PROTEAN TGX™ 4 to 15% gradient gels (Bio-Rad) and underwent electrophoretic separation. Proteins were then transferred to PolyScreen PVDF Hybridization Transfer Membranes (PerkinElmer, Waltham, MA). Membranes were cut according to the molecular weights of the proteins of interest (as indicated with Precision Plus Protein Dual Color, Bio-Rad) and incubated with primary and horseradish peroxidase (HRP)–conjugated secondary antibodies. We used Super Signal West Pico chemiluminescent substrate (Thermo Fisher Scientific) and the ChemiDoc™ imaging system (Bio-Rad), using Image Lab™ software (Bio-Rad) for image file export. Images were processed with Adobe Photoshop Creative Cloud (auto contrast function).
Quinn WJ I.I.I., Jiao J., TeSlaa T., Stadanlick J., Wang Z., Wang L., Akimova T., Angelin A., Schäfer P.M., Cully M.D., Perry C., Kopinski P.K., Guo L., Blair I.A., Ghanem L.R., Leibowitz M.S., Hancock W.W., Moon E.K., Levine M.H., Eruslanov E.B., Wallace D.C., Baur J.A, & Beier U.H. (2020). Lactate Limits T Cell Proliferation via the NAD(H) Redox State. Cell reports, 33(11), 108500.
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2

Western Blot Analysis of Activated T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified stimulated Tconv were lysed in radioimmunoprecipitation assay (RIPA) buffer with Halt protease inhibitor (Thermo Fisher Scientific). Protein concentration was determined by photometry (DU640, Beckman-Coulter). Subsequently, samples were mixed with Laemmli sample buffer containing 2-mercaptoethanol (Bio-Rad Laboratories, Hercules, CA) and boiled for 5 min. The protein samples were loaded onto Mini-PROTEAN TGX™ 4 to 15% gradient gels (Bio-Rad) and underwent electrophoretic separation. Proteins were then transferred to PolyScreen PVDF Hybridization Transfer Membranes (PerkinElmer, Waltham, MA). Membranes were cut according to the molecular weights of the proteins of interest (as indicated with Precision Plus Protein Dual Color, Bio-Rad) and incubated with primary and horseradish peroxidase (HRP)–conjugated secondary antibodies. We used Super Signal West Pico chemiluminescent substrate (Thermo Fisher Scientific) and the ChemiDoc™ imaging system (Bio-Rad), using Image Lab™ software (Bio-Rad) for image file export. Images were processed with Adobe Photoshop Creative Cloud (auto contrast function).
Quinn WJ I.I.I., Jiao J., TeSlaa T., Stadanlick J., Wang Z., Wang L., Akimova T., Angelin A., Schäfer P.M., Cully M.D., Perry C., Kopinski P.K., Guo L., Blair I.A., Ghanem L.R., Leibowitz M.S., Hancock W.W., Moon E.K., Levine M.H., Eruslanov E.B., Wallace D.C., Baur J.A, & Beier U.H. (2020). Lactate Limits T Cell Proliferation via the NAD(H) Redox State. Cell reports, 33(11), 108500.
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