The culture media, FBS, and chemical compounds were purchased from Life Technologies (Grand Island, NY, USA). Antibodies against phospho-EGFR (Tyr1068), phospho-STAT3 (Tyr705), phospho-AKT (Ser473), phosphor-ERK (Thr202/Tyr204), caspase 9, and poly (ADP-ribose) polymerase (PARP) (Asp214) were purchased from Cell Signaling (Temecula, CA, USA). Anti-EGFR, anti-STAT3, anti-AKT, anti-ERK, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Cycloheximide and MG132 were purchased from Sigma (St. Louis, MO, USA).
Chemical compounds
Manufactured by Thermo Fisher Scientific
Sourced in United States
Chemical compounds are substances composed of two or more different chemical elements. They are used in various laboratory applications to facilitate chemical reactions, separations, and analyses. The core function of chemical compounds is to serve as reagents, solvents, or analytical standards in scientific research and testing.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Anti egfr, by Santa Cruz Biotechnology (1 mentions)
Anti erk, by Santa Cruz Biotechnology (1 mentions)
Anti stat3, by Santa Cruz Biotechnology (1 mentions)
Anti akt, by Santa Cruz Biotechnology (1 mentions)
Mg132, by Merck Group (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Nhs activated sepharose 4b, by GE Healthcare (1 mentions)
Culture media, by Thermo Fisher Scientific (1 mentions)
Antibodies against e cadherin, by BD (1 mentions)
Hnrnpa2 b1, by Santa Cruz Biotechnology (1 mentions)
β actin, by BD (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Hepg2, by American Type Culture Collection (1 mentions)
4 protocols using chemical compounds
1
Signaling Pathway Analysis in Cells
2
Investigating AKT Signaling Inhibition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Culture media, chemical
compounds, and fetal bovine serum (FBS) were
purchased from Life Technologies (Grand Island, NY, USA). Antibodies
against phospho-AKT (Ser473) and geldanamycin were purchased from
Cell Signaling (Temecula, CA, USA); antibodies against AKT, Slug,
and hnRNP A2/B1 were purchased from Santa Cruz Biotechnology (Santa
Cruz, CA, USA); antibodies against HSP90 and HSP70 were purchased
from Enzo Life Sciences (Farmingdale, NY, USA); and antibodies against
E-cadherin and β-actin were purchased from BD Sciences (San
Jose, CA, USA) and Sigma (St Louis, MO, USA), respectively. The HSP90
inhibitor 17AAG was purchased from Calbiochem (Gibbstown, NJ, USA).
The phenanthrene-based derivatives PBT-1 and PBT-1-6L (see Figure4 A for their structures) were synthesized in the
laboratory of Dr. K. H. Lee. NHS-activated Sepharose 4B was obtained
from GE Healthcare (Piscataway, NJ, USA). All oligonucleotides were
obtained from Bio Basic (Markham, ON, Canada).
compounds, and fetal bovine serum (FBS) were
purchased from Life Technologies (Grand Island, NY, USA). Antibodies
against phospho-AKT (Ser473) and geldanamycin were purchased from
Cell Signaling (Temecula, CA, USA); antibodies against AKT, Slug,
and hnRNP A2/B1 were purchased from Santa Cruz Biotechnology (Santa
Cruz, CA, USA); antibodies against HSP90 and HSP70 were purchased
from Enzo Life Sciences (Farmingdale, NY, USA); and antibodies against
E-cadherin and β-actin were purchased from BD Sciences (San
Jose, CA, USA) and Sigma (St Louis, MO, USA), respectively. The HSP90
inhibitor 17AAG was purchased from Calbiochem (Gibbstown, NJ, USA).
The phenanthrene-based derivatives PBT-1 and PBT-1-6L (see Figure
laboratory of Dr. K. H. Lee. NHS-activated Sepharose 4B was obtained
from GE Healthcare (Piscataway, NJ, USA). All oligonucleotides were
obtained from Bio Basic (Markham, ON, Canada).
3
Culturing human liver and skin cell lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human HCC cell lines Huh7 and HepG2 were purchased from the American Type Culture Collection (Manassas, VA, USA) and donated by Dr. Chau-Ting Yeh of Chang Gung Memorial Hospital, respectively. Human skin fibroblasts (HFBs) were kindly provided by Dr. Pan-Chyr Yang of Taiwan University. The cells were maintained in Dulbecco's Modified Eagle Medium (Gibco, Gaithersburg, MD, USA) containing 10% fetal bovine serum (FBS) and cultured at 37°C with 5% carbon dioxide in a humidified incubator. Culture medium, chemical compounds, and FBS were purchased from Life Technologies (Grand Island, NY, USA).
4
Culturing Tumorigenic and Non-Tumorigenic OSCC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell Culture SAS is a high-grade tumorigenic human tongue squamous cell carcinoma [18] . OECM1 is a nontumorigenic Taiwanese OSCC cell line. SAS and OECM1 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS), 0.5 mM sodium pyruvate, 1.2 g/L sodium bicarbonate, and 2.5 mM L-glutamine. Culture medium, chemical compounds, and FBS were purchased from Life Technologies (Grand Island, NY).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!