5x106 CAR-T-cells were lysed and 10 μg of protein were separated on a 10% polyacrylamide gel. After blotting, membranes were incubated with an anti-CD247 mAb (BD Biosciences, #556366) followed by an HRP-conjugated rabbit anti-mouse secondary Ab (Cell Signaling Technology, #7076S). To remove N-linked glycosylations, protein lysates were incubated with PNGaseF (NEB) for 1 h at 37°C.
Hrp conjugated rabbit anti mouse secondary ab
Manufactured by Cell Signaling Technology
The HRP-conjugated rabbit anti-mouse secondary Ab is a laboratory reagent used to detect the presence of mouse primary antibodies in various immunoassays. It consists of a rabbit-derived secondary antibody that is conjugated to the enzyme horseradish peroxidase (HRP). This HRP-conjugated secondary antibody binds to the mouse primary antibody and can be used to generate a colorimetric or chemiluminescent signal, allowing for the detection and quantification of the target analyte.
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2 protocols using hrp conjugated rabbit anti mouse secondary ab
1
CD247 Expression in CAR-T Cells
2
CD247 Expression in CAR-T Cells
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5x106 CAR-T-cells were lysed and 10 μg of protein were separated on a 10% polyacrylamide gel. After blotting, membranes were incubated with an anti-CD247 mAb (BD Biosciences, #556366) followed by an HRP-conjugated rabbit anti-mouse secondary Ab (Cell Signaling Technology, #7076S). To remove N-linked glycosylations, protein lysates were incubated with PNGaseF (NEB) for 1 h at 37°C.
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