Rosemary extracts (Kunming Pharmaceutical Co., China.) are the crucial active constituents extracted from Rosmarinus officinalis Linn, containing 60% carnosic acid. RE were dissolved in 1% Tween-80 to prepare a suspension with the concentration of 100 mg/mL. Rabbit anti-BDNF, rabbit anti-Iba1 and rabbit anti-IL-1β were purchased from Abcam (Shanghai, China). Rabbit anti-TNF-α, rabbit anti-p-NF κ B p65, rabbit anti-NF κ B p65, rabbit anti-AKT, rabbit anti-p-AKT 473, β-actin and horseradish peroxidase conjugated anti-rabbit IgG were acquired from Cell Signaling Technology (Boston, MA, United States). Enzyme-linked immunosorbent assay (ELISA) kits were obtained from RD, Bio-Techne (Emeryville, CA, United States). E.Z.N.A. stool DNA Kit was purchased from Omega Bio-tek (Norcross, GA, United States), AxyPrep DNA Gel Extraction Kit was acquired from Axygen Biosciences (Union City, CA, United States).
Rabbit anti p akt 473
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-p-AKT 473 is a primary antibody that specifically recognizes the phosphorylated form of AKT (Protein Kinase B) at serine 473. This antibody is a tool used to detect and quantify the activated form of AKT in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti akt, by Cell Signaling Technology (2 mentions)
Rabbit anti il 1β, by Abcam (1 mentions)
Rabbit anti tnf α, by Cell Signaling Technology (1 mentions)
Rabbit anti iba1, by Abcam (1 mentions)
Rabbit anti nf κb p65, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated anti rabbit igg, by Cell Signaling Technology (1 mentions)
Rabbit anti p nf κb p65, by Cell Signaling Technology (1 mentions)
Rabbit anti bdnf, by Abcam (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Axyprep dna gel extraction kit, by Corning (1 mentions)
E z n a stool dna kit, by Omega Bio-Tek (1 mentions)
Mouse anti sfrp5, by Santa Cruz Biotechnology (1 mentions)
Mouse anti actin, by Santa Cruz Biotechnology (1 mentions)
Ecl advance, by Cell Signaling Technology (1 mentions)
Mouse anti tubulin, by Merck Group (1 mentions)
Quantity one 4, by Bio-Rad (1 mentions)
2 protocols using rabbit anti p akt 473
1
Rosemary Extract Neuroprotective Mechanisms
2
Quantitative Analysis of Protein Abundance
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The concentrations of proteins in the islets and INS-1 cell lysates were quantitated by BCA protein assay (Pierce). Nuclear and cytoplasmic lysates were prepared with the NE-PER Kit (Pierce) according to the manufacturer's instructions. Protein samples (20 μg) were separated by electrophoresis with an 8% SDS-PAGE gel and transferred onto a polyvinylidene fluoride membrane, followed by immunoblotting according to the protocol outlined by Cell Signaling Technology. The blotted membrane was developed with ECL Advance (Cell Signaling) and imaged with a LAS-4000 Super CCD Remote Control Science Imaging System (GE). The relative abundance was quantified by densitometry using Quantity One 4.6.2 software (Bio-Rad). The following antibodies were used: mouse anti-SFRP5 (1:500, Santa Cruz Biotech), mouse anti-cyclin D2 (1:700, Abcam), rabbit anti-pAKT(473) (1:1000, Cell Signaling), rabbit anti-AKT (1:1000, Cell Signaling), mouse anti-tubulin (1:20,000, Sigma-Aldrich), and mouse anti-actin (1:1000, Santa Cruz).
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