HeLa cells (CRUK-CI Biorepository and Cell Services Core) were cultured in DMEM (Sigma, D6429) supplemented with 10 % heat inactivated FBS. U2OS AID-DIvA cells were cultured in DMEM supplemented with 10 % heat inactivated FBS and 800 μg/mL G418 Sulfate (Gibco, 10131). To induce nuclear localization of AsiSI, AID-DIvA cells were treated with 300 nM 4OHT (Sigma, H7904) for 4 h. Normal human epidermal keratinocytes (NHEK), pooled from multiple donors, were purchased from Thermofisher (A13401) and cultured in EpiLife medium (Thermofisher, M-EPI-500-CA) supplemented with human keratinocyte growth supplement (HKGS) (Thermofisher, S-001-5). NHEKs were detached using accutase (Sigma, A6964). HeLa and U2OS AID-DIvA cells were STR genotyped and mycoplasma tested. NHEK cells were obtained from Thermofisher and were certified as mycoplasma negative.
Nhek cells
Manufactured by Thermo Fisher Scientific
NHEK cells are normal human epidermal keratinocytes, which are primary cells derived from human skin. They are commonly used in research to study skin biology, cell signaling, and other cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions)
Epilife medium, by Thermo Fisher Scientific (2 mentions)
Accutase, by Merck Group (2 mentions)
G418 sulfate, by Thermo Fisher Scientific (2 mentions)
Nheks, by Merck Group (2 mentions)
4 oht, by Merck Group (2 mentions)
2 protocols using nhek cells
1
Cell Culture Protocols for HeLa, U2OS, and NHEK
2
Cell Culture Protocols for HeLa, U2OS, and NHEK
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HeLa cells (CRUK-CI Biorepository and Cell Services Core) were cultured in DMEM (Sigma, D6429) supplemented with 10 % heat inactivated FBS. U2OS AID-DIvA cells were cultured in DMEM supplemented with 10 % heat inactivated FBS and 800 μg/mL G418 Sulfate (Gibco, 10131). To induce nuclear localization of AsiSI, AID-DIvA cells were treated with 300 nM 4OHT (Sigma, H7904) for 4 h. Normal human epidermal keratinocytes (NHEK), pooled from multiple donors, were purchased from Thermofisher (A13401) and cultured in EpiLife medium (Thermofisher, M-EPI-500-CA) supplemented with human keratinocyte growth supplement (HKGS) (Thermofisher, S-001-5). NHEKs were detached using accutase (Sigma, A6964). HeLa and U2OS AID-DIvA cells were STR genotyped and mycoplasma tested. NHEK cells were obtained from Thermofisher and were certified as mycoplasma negative.
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