Mass spectrometry grade formic acid

Manufactured by Merck Group

Sourced in United States, Germany

Mass spectrometry grade formic acid is a high-purity chemical reagent used in analytical applications, particularly in mass spectrometry. It serves as a mobile phase additive to improve the ionization and separation of analytes during mass spectrometric analysis.

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Lab products found in correlation

Hplc grade acetonitrile, by Thermo Fisher Scientific (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Nobiletin, by Merck Group (2 mentions) Ms grade acetonitrile, by Thermo Fisher Scientific (2 mentions) Milli q system, by Merck Group (2 mentions) Naringenin, by Merck Group (2 mentions) Tween 80, by Avantor (2 mentions) Methanol meoh high performance liquid chromatography grade, by Merck Group (2 mentions) Amb api powder, by Cayman Chemical (2 mentions) Nah2po4, by Thermo Fisher Scientific (2 mentions) Mgcl2, by Merck Group (2 mentions) Na2hpo4, by Thermo Fisher Scientific (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Cacl2, by Merck Group (2 mentions) Methanol, by Avantor (1 mentions) Hplc grade acetonitrile, by Avantor (1 mentions) Acetonitrile trifluroacetic acid water, by Agilent Technologies (1 mentions) Methionine, by Merck Group (1 mentions) Asparagine, by Merck Group (1 mentions) Alanine, by Merck Group (1 mentions)

9 protocols using mass spectrometry grade formic acid

HPLC-MS Calibration Solution Preparation

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High-Performance Liquid Chromatography (HPLC) grade acetonitrile, methanol and water were purchased from Burdick & Jackson (Morristown, NJ). Mass spectrometry grade formic acid and standards were purchased from Sigma- Aldrich (St. Louis, MO). The calibration solution containing multiple calibrants in acetonitrile/trifluroacetic acid/water was purchased from (Agilent Technologies, Santa Clara, CA).

Jin F., Bhowmik S.K., Putluri V., Gu F., Gohlke J., Von Rundstedt F.C., Dasgupta S., Krishnapuram R., O’Malley B.W., Sreekumar A, & Putluri N. (2015). A Novel [15N] Glutamine Flux using LC-MS/MS-SRM for Determination of Nucleosides and Nucleobases. Journal of analytical & bioanalytical techniques, 6(5), 267.

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Amino Acid Standards Preparation

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In total, 27 amino acid standards including norvaline internal standard were obtained from Sigma (St. Louis, MO, USA). Stock solutions (500 ng/μL) of every compound were prepared and stored at -80 ºC. A 10-point calibration curve (0.01-100 pmol/µL) was constructed by serial dilutions as well as spiked with internal standards at a final concentration (5 pmol/μL). HPLC grade acetonitrile from Fisher Scientific (Fair Lawn, NJ, USA) and Mass spectrometry grade formic acid was obtained from Sigma-Aldrich (St Louis, MO, USA).

Saeed M., Masood Quraishi U., Mustafa G., Farooqi A., Greger M, & Naseem Malik R. (2024). Metabolomics profiling reveals the detoxification and tolerance behavior of two bread wheat (Triticum aestivum L.) varieties under arsenate stress. Food chemistry, 443.

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Amino Acid Standards for Metabolomic Analysis

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Amino acid standards (alanine, arginine, aspartic acid, cysteine,
glutamic acid, glycine, histidine, iso leucine, leucine, lysine, methionine,
phenyl alanine, proline, serine, threonine, tyrosine, valine, glutamine,
asparagine, GABA, citrulline, ornithine, taurine, tryptophan, 5-HTP,
norvaline, and kynurenine) were purchased from Sigma (St. Louis, MO).
Isotopically labeled amino acid standards (alanine (¹³C₃,¹⁵N), arginine (¹³C₆, ¹⁵N₄), aspartic acid (¹³C₄, ¹⁵N), cystine (¹³C₆, ¹⁵N₂), glutamic acid (¹³C₅, ¹⁵N), glycine (¹³C₂, ¹⁵N), histidine
(¹³C₆, ¹⁵N₃), iso leucine
(¹³C₆, ¹⁵N), leucine (¹³C₆, ¹⁵N), lysine (¹³C₆, ¹⁵N₂), methionine (¹³C₅, ¹⁵N), phenyl alanine (¹³C₉, ¹⁵N), proline (¹³C₅, ¹⁵N),
serine (¹³C₃, ¹⁵N), threonine (¹³C₄, ¹⁵N), tyrosine (¹³C₉, ¹⁵N), valine (¹³C₅, ¹⁵N), citrulline (d4), GABA (¹³C₄), glutamine
(¹³C₅), asparagine(¹³C₄), ornithine (d6), tryptophan (d8), kynurenine (d4)) were obtained
from Cambridge Isotope Laboratories (Andover, MA). Stock solutions
of each compound were prepared at a concentration of 500 ng/μL
and stored at −80 °C. A 10-point calibration curve (0.01–100
pmol/μL) was prepared by serial dilutions and spiked with internal
standards at a final concentration of 5 pmol/μL. Mass spectrometry-grade
formic acid was purchased from Sigma-Aldrich (St Louis, MO), and HPLC-grade
acetonitrile was purchased from Fisher Scientific (Fair Lawn, NJ).

Spain O, & Funk C. (2022). Detailed Characterization of the Cell Wall Structure and Composition of Nordic Green Microalgae. Journal of Agricultural and Food Chemistry, 70(31), 9711-9721.

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High-Sensitivity Mass Spectrometry Workflow

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Ekspert nanoLC 400 series® liquid chromatography system (SCIEX, USA), a TripleTOF® 6600 mass spectrometer (SCIEX, USA), a high-speed refrigerated centrifuge 5810R (Eppendorf, Germany), and the - 80 ℃ refrigerator (Thermo, USA) were the instrument used for this study. The reagents used included mass spectrometry grade acetonitrile, methanol (Thermo Fisher Scienti c, USA), mass spectrometry grade formic acid (Sigma-Aldrich, USA), and puri ed water (Mass spectrometric grade).

Ding X., Tong R., Song H., Sun G., Wang D., Liang H., Sun J., Cui Y., Zhang X., Liu S., Cheng M., & Sun T. (2021). Construction of the Metabolomics-Based Prognosis-Prediction Models for ICU Septic Patients.

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Quantification of Bioactive Compounds in CRP Tea

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CRP samples (Batch number: 201712) were acquired from Xinhui Hele Tea Art Co. Ltd. (Jiangmen, China), and were authenticated by Prof. Wenbo Liao from Sun Yat-sen University. Corresponding voucher specimens were kept in our laboratory. Reserpine injection was purchased from Guangdong Bangmin Pharmaceutical Co., Ltd. (Jiangmen, China). The reference standards of hesperidin, naringin, neohesperidin and rutin were obtained from National Institute for Control of Biological and Pharmaceutical Products of China (Beijing, China). Hesperetin, naringenin, nobiletin and mass spectrometry (MS) grade formic acid were purchased from Sigma-Aldrich (St. Louis, MO, United States). MS grade acetonitrile was purchased from Fisher Scientific (Pittsburgh, PA, United States). All water used was distilled and further purified by a Milli-Q system (Millipore, Milford, MA, United States). Other reagents used in the experiment were of analytical grade.

Zheng Y., Zeng X., Chen P., Chen T., Peng W, & Su W. (2020). Integrating Pharmacology and Gut Microbiota Analysis to Explore the Mechanism of Citri Reticulatae Pericarpium Against Reserpine-Induced Spleen Deficiency in Rats. Frontiers in Pharmacology, 11, 586350.

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Comprehensive Chemical Analysis of Ganpu Tea

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Samples of Ganpu tea and the corresponding raw materials (including Pu-erh tea and GCP) were provided by Xinhui Hele Tea Art Co. Ltd. (Jiangmen, China). The reference standards of gallic acid, caffeine, hesperidin, naringin, neohesperidin, rutin, rhoifolin, and synephrine were obtained from the National Institute for Control of Biological and Pharmaceutical Products of China (Beijing, China). Naringenin, poncirin, nobiletin, sinensetin, tangeretin, and mass spectrometry (MS) grade formic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hesperetin and N-methyltyramine were acquired from Sinova (Shenzhen, China). MS-grade acetonitrile was purchased from Fisher Scientific (Pittsburgh, PA, USA). Water used in the experiment was distilled and further purified with a Milli-Q system (Millipore, Milford, MA, USA). All other reagents used were of analytical grade.

Zheng Y., Zeng X., Chen T., Peng W, & Su W. (2020). Chemical Profile, Antioxidative, and Gut Microbiota Modulatory Properties of Ganpu Tea: A Derivative of Pu-erh Tea. Nutrients, 12(1), 224.

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Purchasing Solvents and Dye for Analysis

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Methanol (MeOH; UPLC grade) and isopropanol (HPLC grade) were purchased from AppliChem (Darmstadt, Germany), acetonitrile (HPLC grade) was purchased from Roth (Karlsruhe, Germany), and formic acid (mass spectrometry grade) from Sigma-Aldrich (Hamburg, Germany). Hoechst 33342 was purchased from Life Technologies (Darmstadt, Germany).

Muschet C., Möller G., Prehn C., de Angelis M.H., Adamski J, & Tokarz J. (2016). Removing the bottlenecks of cell culture metabolomics: fast normalization procedure, correlation of metabolites to cell number, and impact of the cell harvesting method. Metabolomics, 12(10), 151.

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Analytical Characterization of Amphotericin B

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AmB analytical standard (87.8%), methanol (MeOH) high performance liquid chromatography (HPLC) grade, formic acid mass spectrometry grade, NaOH, MgCl 2 , CaCl 2 , CTAB, and NaHCO 3 were obtained from Sigma Aldrich (Germany); AmB API powder (85%) from Cayman Chemical (USA); BSA protease free powder fraction V, dimethyl sulfoxide (DMSO), dextrose, SLS, Na 2 HPO 4 , NaH 2 PO 4 , KH 2 PO 4 , NaCl and KCl from Fisher Scientific (USA); Tween 80 from Amresco (USA); GF/D (pore size 2.7 μm, 25 mm diameter) and GF/F (pore size 0.7 μm, 25 mm diameter) filters from Whatman (UK); regenerated cellulose (RC) filters 0.45 µm 13 mm diameter from Cronus (UK); cellulose ester dialysis tubing of 300 kDa MWCO from Spectrum Labs® (USA) and Sep -Pak® Vac 3cc (500 mg) tC18 SPE column from Waters (Massachusetts, USA).

Díaz de León-Ortega R., D'Arcy D.M., Lamprou D.A., Xue W.F, & Fotaki N. (2021). In vitro in vivo relations for the parenteral liposomal formulation of Amphotericin B: A biorelevant and clinically relevant approach. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 159.

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Analytical Standard Characterization Protocol

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AmB analytical standard (87.8%), methanol (MeOH) high performance liquid chromatography (HPLC) grade, formic acid mass spectrometry grade, NaOH, MgCl2, CaCl2, hexadecyltrimethylammonium bromide (CTAB), cholesterol (CH), NHCO3 and NH4HCO2 were obtained from Sigma Aldrich (Germany); ethylenediaminetetracetic acid anhydrous (EDTA) from Sigma Aldrich (USA); AmB API powder (85%) from Cayman Chemical (USA); BSA protease free powder fraction V, dimethyl sulfoxide (DMSO), dextrose, sodium dodecyl sulphate (SLS), Na2HPO4, NaH2PO4, KH2PO4, NaCl and KCl from Fisher Scientific (USA); Tween 80 from Amresco (USA); phosphatidylcholine from egg from Lipoid GmbH (Germany); sodium taurocholate from Prodotti Chimici e Alimentaria (Italy); GF/D (pore size 2.7 μm, 25 mm diameter) and GF/F (pore size 0.7 μm, 25 mm diameter) filters from Whatman (UK) and regenerated cellulose (RC) filters 0.45 µm 13 mm diameter from Cronus (UK).

Díaz de León-Ortega R., D'Arcy D.M, & Fotaki N. (2020). In vitro conditions for performance evaluation of products for intravascular administration: Developing appropriate test media using Amphotericin B as a model drug. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 143.

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