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Alexa fluor 546 conjugated goat anti rat igg h l antibody

Manufactured by Merck Group

Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody is a secondary antibody used for detection and visualization in immunoassays. It is conjugated with the fluorescent dye Alexa Fluor 546 and specifically binds to the heavy and light chains of rat immunoglobulin G (IgG).

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2 protocols using alexa fluor 546 conjugated goat anti rat igg h l antibody

1

Immunofluorescence Labeling of E-cadherin and SOX9

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The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (#13-1900, 1:50 dilution, Thermo Fischer Scientific), anti-SOX9 rabbit antibody (#AB5535-25UG, 1:200 dilution, Merck Millipore), Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (#A11081, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rat IgG (H+L) antibody (#A21247, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (#A32733, 1:1000 dilution)(all Thermo Fisher Scientific).
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2

Immunofluorescence Imaging of E-cadherin and SOX9

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The following primary and secondary antibodies were used for immunofluorescence: anti-Ecadherin rat antibody (#3195, 1:100 dilution, Cell Signaling Technology), anti-SOX9 rabbit antibody (#AB5535-25UG, 1:200 dilution, Merck Millipore), Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (#A11081, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rat IgG (H+L) antibody (#A21247, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (#A32733, 1:1000 dilution)(all Thermo Fisher Scientific).
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