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Hpa006111

Manufactured by Abcam

HPA006111 is a polyclonal antibody that recognizes phosphorylated serine residues. It is produced in rabbit and can be used for various applications, such as western blotting, immunohistochemistry, and immunocytochemistry.

Automatically generated - may contain errors

2 protocols using hpa006111

1

Protein Extraction and Western Blot Analysis

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Tissues were lysed in RIPA buffer containing 0.1% SDS, 1% NP-40, 0.5% Na deoxycholate, 150 mM NaCl, 50 mMTris-Cl (pH 8.0), and 1 mMEDTA supplemented with protease inhibitor (Roche). Total proteins were loaded and fractionated by SDS-PAGE, transferred to a nitrocellulose membrane, and detected with the antibodies anti-NFIA (1:500 dilution, Sigma, HPA006111), anti-Ox-Phos (1:1,000 dilution, Abcam, 110413), anti-actin (1:1,000 dilution, Santa Cruz Biotechnology, sc-1616), anti-NF-κB p65 (1:1,000 dilution, Cell Signaling, 8242), and FLAG M2 (1:2,000 dilution, Sigma, F3165).
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2

Western Blot Analysis of Protein Targets

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Tissues were lysed in radioimmunoprecipitation assay (RIPA) buffer containing 0.1% SDS, 1% NP-40, 0.5% Na deoxycholate, 150 mM NaCl, 50 mM Tris-Cl (pH 8.0), 1 mM EDTA supplemented with protease inhibitor (Roche). Proteins were separated by SDS-PAGE, transferred to nitrocellulose membrane, and detected with the antibodies anti-NFIA (1:1000 dilution, Sigma HPA006111), anti-UCP1 (1:2000 dilution, Abcam, ab10983), anti-VDR (1:100 dilution, Santa Cruz Biotechnology, sc-13133), anti-FLAG M2 (Sigma F3165), and anti-β actin (Sigma A3854).
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