Hpa006111

Manufactured by Abcam

HPA006111 is a polyclonal antibody that recognizes phosphorylated serine residues. It is produced in rabbit and can be used for various applications, such as western blotting, immunohistochemistry, and immunocytochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Anti nfia, by Merck Group (1 mentions) Anti oxphos, by Abcam (1 mentions) Anti nf κb p65, by Cell Signaling Technology (1 mentions) Anti actin, by Santa Cruz Biotechnology (1 mentions) Flag m2, by Merck Group (1 mentions) Ab10983, by Santa Cruz Biotechnology (1 mentions) Anti vdr, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti flag m2, by Merck Group (1 mentions)

2 protocols using hpa006111

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissues were lysed in RIPA buffer containing 0.1% SDS, 1% NP-40, 0.5% Na deoxycholate, 150 mM NaCl, 50 mMTris-Cl (pH 8.0), and 1 mMEDTA supplemented with protease inhibitor (Roche). Total proteins were loaded and fractionated by SDS-PAGE, transferred to a nitrocellulose membrane, and detected with the antibodies anti-NFIA (1:500 dilution, Sigma, HPA006111), anti-Ox-Phos (1:1,000 dilution, Abcam, 110413), anti-actin (1:1,000 dilution, Santa Cruz Biotechnology, sc-1616), anti-NF-κB p65 (1:1,000 dilution, Cell Signaling, 8242), and FLAG M2 (1:2,000 dilution, Sigma, F3165).

Hiraike Y., Saito K., Oguchi M., Wada T., Toda G., Tsutsumi S., Bando K., Sagawa J., Nagano G., Ohno H., Kubota N., Kubota T., Aburatani H., Kadowaki T., Waki H., Yanagimoto S, & Yamauchi T. (2023). NFIA in adipocytes reciprocally regulates mitochondrial and inflammatory gene program to improve glucose homeostasis. Proceedings of the National Academy of Sciences of the United States of America, 120(31), e2308750120.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissues were lysed in radioimmunoprecipitation assay (RIPA) buffer containing 0.1% SDS, 1% NP-40, 0.5% Na deoxycholate, 150 mM NaCl, 50 mM Tris-Cl (pH 8.0), 1 mM EDTA supplemented with protease inhibitor (Roche). Proteins were separated by SDS-PAGE, transferred to nitrocellulose membrane, and detected with the antibodies anti-NFIA (1:1000 dilution, Sigma HPA006111), anti-UCP1 (1:2000 dilution, Abcam, ab10983), anti-VDR (1:100 dilution, Santa Cruz Biotechnology, sc-13133), anti-FLAG M2 (Sigma F3165), and anti-β actin (Sigma A3854).

Hiraike Y., Tsutsumi S., Wada T., Oguchi M., Saito K., Nakamura M., Ota S., Koebis M., Nakao H., Aiba A., Nagano G., Ohno H., Oki K., Yoneda M., Kadowaki T., Aburatani H., Waki H, & Yamauchi T. (2022). NFIA determines the cis-effect of genetic variation on Ucp1 expression in murine thermogenic adipocytes. iScience, 25(8), 104729.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!