Canine pancreatic rough microsomes were a kind gift from Prof. B. Dobberstein and stored at 2 eq/µl in RM buffer (250 mM sucrose, 50 mM Hepes/KOH pH 7.6, 50 mM KOAc, 2 mM Mg(OAc)2, 1 mM dithiothreitol (DTT)). Primary antibodies: rabbit anti-mCherry (Invitrogen, PA534974, 1:5000), rabbit anti-calnexin (Enzo Life Sciences, ADI-SPA-865-F, 1:2000), mouse anti-tubulin (Sigma-Aldrich, T6199-100UL,1:10,000), rabbit anti-his-tag (Cell Signaling, 2365S, 1:2,000) and mouse anti-S-peptide (Invitrogen, MA1-981,1:3000). IRDye conjugated secondary antibodies against rabbit (680LT, 926-68021, 1:20,000) and mouse (800CW, 926-32210, 1:15,000) were obtained from LiCor Biosciences.
Rabbit anti his tag
Manufactured by Cell Signaling Technology
Rabbit anti-his-tag is a primary antibody used for the detection and purification of recombinant proteins containing a histidine (His) tag. It recognizes the His-tag sequence with high specificity and sensitivity.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti myc, by Merck Group (1 mentions)
Rabbit anti erk, by Cell Signaling Technology (1 mentions)
Mouse anti gapdh, by Merck Group (1 mentions)
Mouse anti phospho erk1 2, by Cell Signaling Technology (1 mentions)
Rabbit anti flag, by Merck Group (1 mentions)
Rabbit anti pak1, by Cell Signaling Technology (1 mentions)
Rabbit anti gfp, by Merck Group (1 mentions)
Mouse anti flag, by Merck Group (1 mentions)
2 protocols using rabbit anti his tag
1
Canine Pancreatic Microsome Preparation and Antibody Characterization
2
Western Blot Protein Detection Protocol
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Western blots were performed as described previously (McGarry et al., 2016) (link). The following primary antibodies were used in the study; rabbit anti-FLAG (F7425, Sigma), mouse anti-FLAG (F1804, Sigma), mouse anti-myc (M4439, Sigma), rabbit anti-MICAL1 (41112S, Cell Signaling), rabbit anti-phospho-PAK1 T423 (2601S, Cell Signaling), rabbit anti-phospho-PAK1 S144 (2606S, Cell Signaling), rabbit anti-PAK1 (2602S, Cell Signaling), mouse anti-Rab7 (95746S, Cell Signaling), mouse anti-GAPDH (MAB374, Millipore), rabbit anti-phospho-RxxS*/T* (9614S, Cell Signaling), mouse anti-phospho ERK1/2 (9106S, Cell Signaling), rabbit anti-ERK (9102S, Cell Signaling), rabbit anti-GFP (G1544, Sigma), rabbit anti-His-Tag (2365S, Cell Signaling), rabbit anti-GST-Tag (2622S, Cell Signaling). The secondary antibodies used were IRDyeâ 680RD and 800CW (LiCor). Proteins were visualized by infrared imaging using LiCor Odyssey CLx.
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