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Powerscript

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PowerScript is a high-performance reverse transcription system designed for sensitive and efficient cDNA synthesis. It utilizes a proprietary reverse transcriptase enzyme to ensure reliable and consistent results.

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Superscript 3, by Thermo Fisher Scientific (1 mentions) Primer express software, by Thermo Fisher Scientific (1 mentions)

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PowerScript reverse transcriptase (11 citations) PowerScript RT (2 citations) PowerScript cDNA synthesis strip (2 citations)

2 protocols using powerscript

1

RT-PCR Protocol for RNA Quantification

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RT-PCR was carried out with 0.05–3 µg of total RNA, using either Superscript III (Invitrogen) or PowerScript reverse transcriptase (Clontech) in conjunction with SpAncRT or SMARTIV and CDIII (Clontech) oligonucleotides (Table 1). All RT-PCR procedures were carried out in accordance with manufacturers' protocols. Both the 5′ and 3′ oligonucleotides used in reverse transcription reactions provided binding sites for primers used in PCR amplifications.
Roth M.O., Wilkins A.G., Cooke G.M., Raftos D.A, & Nair S.V. (2014). Characterization of the Highly Variable Immune Response Gene Family, He185/333, in the Sea Urchin, Heliocidaris erythrogramma. PLoS ONE, 9(10), e62079.
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2

Quantifying Vcam1 Gene Expression

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Gene expression was assessed in mouse tissues (heart, lung, aorta and carotid arteries) using RT-qPCR. Total RNA was isolated using the RNeasy Mini plus Isolation Kit (Qiagen). RT into single stranded cDNA was performed using the high capacity random primer reverse-transcription kit (Applied Biosystems, Foster City, CA, or Powerscript, Clontech, Palo Alto, CA). Primers to detect mRNA and hnRNA of wild type and mutant Vcam1 alleles and housekeeping genes (CD31 and HPRT) were designed using the Primer Express software (Applied Biosystems). Primer sequences are in Supplemental Table I.
Milstone D.S., Ilyama M., Chen M., O’Donnell P., Davis V.M., Plutzky J., Brown J.D., Haldar S.M., Siu A., Lau A.C., Zhu S.N., Basheer M.F., Collins T., Jongstra-Bilen J, & Cybulsky M.I. (2015). Differential Role of a NF-kappaB Transcriptional Response Element in Endothelial versus Intimal Cell VCAM-1 Expression. Circulation research, 117(2), 166-177.
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