Buffers and hplc reagents

Methylone hydrochloride, MDPV hydrochloride, (±) mephedrone hydrochloride, and (±) MDMA hydrochloride were obtained from the NIDA Research Resources Drug Supply Program. (+) methamphetamine hydrochloride, D-amphetamine sulfate, MPTP hydrochloride, DA, polyclonal antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and all buffers and HPLC reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bicinchoninic acid protein assay kits were obtained from Pierce (Rockford, IL, USA). Polyclonal antibodies against rat tyrosine hydroxylase (TH) were produced as described previously (Kuhn and Billingsley 1987 (link)). Monoclonal antibodies against rat DAT were generously provided by Dr Roxanne A. Vaughan (University of North Dakota, Grand Forks, ND, USA). Polyclonal antibodies against glial fibrillary acidic protein (GFAP) were obtained from Thermo Scientific (Rockford, IL, USA). IRDye secondary antibodies for Odyssey Imaging Systems were purchased from LiCor Biosciences (Lincoln, NE, USA).

(R,S)-N-Methcathinone HCl and (R,S)-mephedrone HCl were provided by the NIDA Research Resources Drug Supply Program. Racemic 4-methlymethamphetamine HCl was synthesized as described by Davis et al. (2012) (link) from methylamine HCl and 4-methylphenylacetone purchased from Alfa Aesar (Ward Hill, MA, USA). (+)- Methamphetamine HCl, pargyline HCl, L-3,4-dihydroxyphenylalanine (L-DOPA), S-(−)-carbidopa, DA, polyclonal antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and all buffers and HPLC reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bicinchoninic acid protein assay kits for Western blot analysis were obtained from Pierce (Rockford, IL, USA). Polyclonal antibodies against rat TH were produced as previously described (Kuhn and Billingsley, 1987 (link)). Monoclonal antibodies against rat DAT were generously provided by Dr. Roxanne Vaughan (University of North Dakota, Grand Forks, ND, USA). IRDye secondary antibodies for Odyssey Imaging Systems were purchased from LiCor Biosciences (Lincoln, NE, USA).