To isolate total RNA from the thyroid cell culture samples and from the purified exosomes isolated from the conditioned media, TRIzol reagent (Thermo Fisher Scientific Inc., Waltham, MA, USA; code 15596026) and Total Exosome RNA (Thermo Fisher Scientific Inc., Waltham, MA, USA; code 4478545) were used [13 (link)]. RNA levels were quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA) and are as follows: 2.9 ng/µL and 5.8 ng/µL for TPC-1 and Nthy-ori 3.1, respectively.
Total exosome rna
Manufactured by Thermo Fisher Scientific
Sourced in United States
Total Exosome RNA is a lab equipment product that enables the isolation and purification of exosomal RNA from various biological samples. It provides a reliable and efficient method for extracting high-quality exosomal RNA for further analysis and research.
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Lab products found in correlation
Nanodrop 2000, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
2100 bioanalyzer, by Agilent Technologies (1 mentions)
Protein isolation kit, by Thermo Fisher Scientific (1 mentions)
Glycogen, by Thermo Fisher Scientific (1 mentions)
Ribogreen, by Thermo Fisher Scientific (1 mentions)
Agilent rna 6000 pico kit, by Agilent Technologies (1 mentions)
Dna free rna kit, by Zymo Research (1 mentions)
2 protocols using total exosome rna
1
Thyroid Cell RNA Isolation and Quantification
2
Exosome RNA Isolation and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissue was homogenized, and then RNA was isolated using an RNAeasy Easy Lipid Tissue Kit (Qiagen) according to the manufacturer's instructions. Any DNA contamination was removed using the DNA-free RNA Kit (Zymo research). RNA was isolated from exosomes by using Total Exosome RNA and a protein isolation kit (ThermoFisher Scientific) as per the manufacturer's instructions. Ten micrograms of glycogen (ThermoFisher Scientific) was added during the RNA precipitation step after overnight incubation. RNA was eluted in 10-15 μl of nucleic acidfree water supplied along with the aforementioned kit followed by quantification with a RiboGreen (ThermoFisher Scientific) assay according to the manufacturer's instructions. The exosome RNA quality, yield, and size were analyzed using capillary electrophoresis (2100 Bioanalyzer, Agilent Technologies). One microliter of exosome RNA was used with the Agilent RNA 6000 Pico Kit (catalog no. 5067-1513) according to the manufacturer's protocol. Electropherograms were analyzed using the Agilent 2100 Expert B.02.07 software.
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