1 2 dioleoyl sn glycero 3 phosphatidylethanolamine

Sphingomyelin (egg, cicken) (eSM, 860061); L-α-phosphatidylcholine from hen egg yolk (ePC, 840051); 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE, 850725); heart bovine cardiolipin; ≈90% tetralinoleoyl (CL, 840012); ceramide (egg, chicken) (eCer, 860051); and 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine-N-(lissamine rhodamine B sulfonyl) (Rho-PE, 810150) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Methanol and chloroform were obtained from Fisher (Suwanee, GA,USA). The buffer solution, unless otherwise stated, was 20 mM piperazine-N,N’-bis(2-ethanesulfonic acid) (PIPES), 1 mM ethylene diamino tetraacetate (EDTA), 150 mM NaCl, pH 7.4. All salts and organic solvents were of analytical grade, supplied by Sigma (St. Louis, MO, USA).

L-α-phosphatidylcholine from hen egg yolk (ePC, 840051), 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE, 850725), liver phosphatidylinositol (PI, 840042), egg dioleoylglycerol (DOG, 800811), 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine-N-lissamine rhodamine B sulfonyl (Rho-PE, 810150) and 1-oleoyl-2-{6-[(7-nitro-2–1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphoethanolamine (NBDtail-PE, 810155) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphatidylethanolamine (NBD-PE, N360), p-xylene-bis-pyridinium bromide (DPX, X-1525) and 8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt (ANTS, A350) were purchased from Thermo Fisher Scientific (Waltham, MA).

Palmitoyl coenzyme A (p-CoA), oleoyl coenzyme A (o-CoA), palmitoyl lysophosphatidic acid (16:0 LPA), oleoyl lysophosphatidic acid (18:1 LPA), 1,2-dipalmitoyl-sn-glycero-3-phosphate (DPPA), 1,2-dioleoyl-sn-glycero-3-phosphate (DOPA), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DOPG), 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-sn-glycerol (cardiolipin), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000] (DSPE-PEG-biotin) were purchased from Avanti Polar Lipids. N-(6-tetramethylrhodaminethiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (TRITC-DHPE) was from Invitrogen. Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red DHPE), 212 μm-300 μm acid washed glass beads, chloroform, methanol, acetylacetone, glycerol-3-phosphate (G3P), β-mercaptoethanol, and L-serine were from Sigma-Aldrich. Formic acid, ammonium formate and ULC grade organic solvents for mobile phases were from Biosolve. Cytidine triphosphate (CTP) was from Promega.