Dactolisib

Manufactured by LC Laboratories

Sourced in United States

Dactolisib is a chemical compound developed for research purposes. It functions as an inhibitor targeting the phosphoinositide 3-kinase (PI3K) signaling pathway. The product is intended for use in laboratory research applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Essential amino acid, by Thermo Fisher Scientific (2 mentions) Biocoll, by Harvard Bioscience (2 mentions) Mec1 cells, by American Type Culture Collection (2 mentions) Rapamycin, by LC Laboratories (2 mentions) Pictilisib, by LC Laboratories (2 mentions) Wortmannin, by LC Laboratories (2 mentions) Gant61, by Merck Group (2 mentions) Copanlisib, by MedChemExpress (1 mentions) Everolimus, by LC Laboratories (1 mentions) Venetoclax, by MedChemExpress (1 mentions) Imdm medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Gefitinib, by LC Laboratories (1 mentions) Imatinib, by LC Laboratories (1 mentions) Hek293ft, by American Type Culture Collection (1 mentions) Fetal calf serum (fcs), by American Type Culture Collection (1 mentions)

4 protocols using dactolisib

Preparation and Dilution of PI3K Inhibitors

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The PI3K pathway inhibitors vistusertib (HY-15247, MedChem Express), everolimus (E-4040, LC Laboratories), dactolisib (N-4288, LC Laboratories), and sapanisertib (I-3344, LC Laboratories), were dissolved in DMSO to a 10 mmol/L stock concentration. Copanlisib (HY-15346, MedChem Express) was dissolved in 10 mmol/L TFA/DMSO to a 5 mmol/L stock concentration. Described inhibitors were diluted in fresh media at concentrations ranging from 5 nmol/L to 500 nmol/L for diameter studies.

DeStefanis R.A., Kratz J.D., Olson A.M., Sunil A., DeZeeuw A.K., Gillette A.A., Sha G.C., Johnson K.A., Pasch C.A., Clipson L., Skala M.C, & Deming D.A. (2022). Impact of baseline culture conditions of cancer organoids when determining therapeutic response and tumor heterogeneity. Scientific Reports, 12, 5205.

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Culturing GIST-T1 and GIST-T1/670 Cells

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GIST-T1 and GIST-T1/670 were kindly provided by Dr. César Serrano from Vall d’Hebron Institute of Oncology. These cells were cultured in IMDM medium (ThermoFisher Scientific, Waltham, MA, USA) supplemented with 15% of fetal bovine serum (FBS) (10270106, ThermoFisher Scientific), 1% of penicillin/streptomycin (15140122, Gibco ThermoFisher Scientific) and 1% of L-glutamine (25030024, ThermoFisher Scientific) at 37 °C and 5% of CO₂. Imatinib, dactolisib, and gefitinib were obtained from LC Laboratories (Woubourn, MA, USA); venetoclax was purchased at MedChemExpress (Monmouth Junction, NJ, USA), and repretinib from Selleckchem (Munich, Germany). All treatments were diluted in dimethyl sulfoxide (DMSO) (D8418, Sigma-Aldrich, Saint Louis, MO, United States).

Manzano-Muñoz A., Yeste J., Ortega M.A., Martín F., López A., Rosell J., Castro S., Serrano C., Samitier J., Ramón-Azcón J, & Montero J. (2022). Microfluidic-based dynamic BH3 profiling predicts anticancer treatment efficacy. NPJ Precision Oncology, 6, 90.

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CLL Cell Isolation and Culture

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All work with primary human material was performed in compliance with the national and local regulations. Primary CLL cells were isolated from peripheral blood and bone marrow of CLL patients via Biocoll (Biochrom AG) density gradient centrifugation and cultured in RPMI 1640 medium supplemented with 10% FCS, penicillin (62.5 μg/ml) and streptomycin (100 μg/ml) at a density of 1*10⁶ cells/ml. MEC1 cells (ATCC) were cultured in IMDM medium containing 10% FCS, penicillin and streptomycin. Cells were split twice a week at a density of 10⁶ cells per ml medium. For lentiviral packaging, HEK293FT (ATCC) cells were grown in DMEM supplemented with 10% fetal bovine serum (Life Technologies), penicillin and streptomycin, L-glutamate (100x concentrate, Invitrogen) and essential amino acids (100x concentrate, Life Technologies) and split three times a week in a ratio of 1:5. Dactolisib, pictilisib, rapamycin, wortmannin (LC Laboratories) and GANT61 (Merck) were dissolved in DMSO, stored as stock solution at −20 and diluted to final concentrations as indicated in the results section.

Kern D., Regl G., Hofbauer S.W., Altenhofer P., Achatz G., Dlugosz A., Schnidar H., Greil R., Hartmann T.N, & Aberger F. (2015). Hedgehog/GLI and PI3K signaling in the initiation and maintenance of chronic lymphocytic leukemia. Oncogene, 34(42), 5341-5351.

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Primary CLL Cell Isolation and Culture

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All work with primary human material was performed in compliance with the national and local regulations. Primary CLL cells were isolated from PB and BM of CLL patients via Biocoll (Biochrom AG, Berlin, Germany) density gradient centrifugation and cultured in RPMI 1640 medium supplemented with 10% fetal calf serum, penicillin (62.5 μg/ml) and streptomycin (100 μg/ml) at a density of 1 × 10⁶ cells/ml. MEC1 cells (ATCC, Manassas, VA, USA) were cultured in IMDM medium containing 10% fetal calf serum, penicillin and streptomycin. Cells were split twice a week at a density of 10⁶ cells per ml medium. For lentiviral packaging, HEK293FT (ATCC) cells were grown in DMEM supplemented with 10% fetal bovine serum (Life Technologies), penicillin and streptomycin, L-glutamate (100x concentrate, Life Technologies) and essential amino acids (100 × concentrate, Life Technologies) and split three times a week in a ratio of 1:5. Dactolisib, pictilisib, rapamycin, wortmannin (LC Laboratories, Woburn, MA, USA) and GANT61 (Merck, Darmstadt, Germany) were dissolved in dimethylsulfoxide, stored as stock solution at −20 and diluted to final concentrations as indicated in the Results section.

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