Formvar coated copper slot grids

Manufactured by SPI Supplies

Sourced in United States

Formvar-coated copper slot grids are a type of lab equipment used for the preparation and support of samples for electron microscopy. They consist of a copper mesh or grid coated with a thin layer of the polymer Formvar, which provides a stable and uniform substrate for mounting specimens.

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Lab products found in correlation

Tecnai g2 20 twin 200 kv lab6 tem, by Thermo Fisher Scientific (3 mentions) Ultrascan 1000 camera, by Ametek (2 mentions) Gatan ultrascan 1000 camera, by Ametek (1 mentions)

3 protocols using formvar coated copper slot grids

Preparation and Imaging of LR White-embedded Sections

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LR White-embedded ultra-thin sections were collected on 0.35% Formvar-coated copper slot grids (from SPI Supplies in West Chester, PA, United States). Grids were post stained for 3 min with 2% aqueous uranyl acetate and 3 min with 1% KMnO₄. Images were taken with a 4-megapixel Gatan UltraScan 1000 camera (from Gatan in Pleasanton, CA, United States) on a FEI Tecnai G2 20 Twin 200 kV LaB6 TEM (from FEI in Hillsboro, OR, United States) operating at 200 kV.

Nagle N.J., Donohoe B.S., Wolfrum E.J., Kuhn E.M., Haas T.J., Ray A.E., Wendt L.M., Delwiche M.E., Weiss N.D, & Radtke C. (2020). Chemical and Structural Changes in Corn Stover After Ensiling: Influence on Bioconversion. Frontiers in Bioengineering and Biotechnology, 8, 739.

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Transmission Electron Microscopy Imaging

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Ultra-thin sections were positioned on 0.5% formvar coated copper slot grids (SPI Supplies, West Chester, PA, USA). Grids were post-stained for 1 min with 1% aqueous KMnO₄. Images were captured with a 4 megapixel Gatan UltraScan 1000 camera (Gatan, Pleasanton, CA, USA) on a FEI Tecnai G2 20 Twin 200 kV LaB6 TEM (FEI).

Ciesielski P.N., Wang W., Chen X., Vinzant T.B., Tucker M.P., Decker S.R., Himmel M.E., Johnson D.K, & Donohoe B.S. (2014). Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 2: morphological and structural substrate analysis. Biotechnology for Biofuels, 7, 47.

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Lignin Staining of Resin-Embedded Sections

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Thin (60 nm) resin-embedded sections were positioned on 0.5% Formvar-coated copper slot grids (SPI Supplies, West Chester, PA, USA). Grids were post-stained for 6 minutes with 2% aqueous uranyl acetate and for 6 minutes with 1% aqueous KMnO₄ to selectively stain for lignins. Images were taken with a 4 mega-pixel Gatan UltraScan 1000 camera (Gatan, Pleasanton, CA, USA) on a FEI Tecnai G2 20 Twin 200 kV LaB6 TEM (FEI, Hilsboro, OR, USA).

Wang W., Chen X., Donohoe B.S., Ciesielski P.N., Katahira R., Kuhn E.M., Kafle K., Lee C.M., Park S., Kim S.H., Tucker M.P., Himmel M.E, & Johnson D.K. (2014). Effect of mechanical disruption on the effectiveness of three reactors used for dilute acid pretreatment of corn stover Part 1: chemical and physical substrate analysis. Biotechnology for Biofuels, 7, 57.

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