The largest database of trusted experimental protocols

Absolute ethanol

Manufactured by Tianjin Damao
Sourced in China

Absolute ethanol is a pure, anhydrous form of ethanol. It is a clear, colorless liquid with a characteristic odor. Absolute ethanol has a high purity level, typically 99.9% or greater. It serves as a versatile solvent and reagent for various applications in laboratory settings.

Automatically generated - may contain errors

13 protocols using absolute ethanol

1

Synthesis of Silver Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Silver nitrate (AgNO3, 98%) was acquired from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China), sodium chloride (NaCl, 99.9%) was obtained from Sigma–Aldrich (St. Louis, MO, USA)., sodium bromide (NaBr, 99.0%) was provided by Fuchen Chemical Reagent Factory (Tianjin, China), polyvinylpyrrolidone (PVP, MW ≈ 1300000) was supplied by Macklin Biochemical Co., Ltd. (Shanghai, China), ethylene glycol (EG, 99.8%), and absolute ethanol (99.7%) were acquired from Damao Chemical Reagent Factory (Tianjin, China). All of the chemicals were applied without further purification.
+ Open protocol
+ Expand
2

Antioxidant Properties of Monk Fruit Extract

Check if the same lab product or an alternative is used in the 5 most similar protocols
Siraitia grosvenorii seed was bought from Guilin Monk Fruit Corp. Wheat flour was bought from Wudeli Flour Group Co., Ltd. (Xinxiang, China). Yeast was purchased from Angel Yeast Co., Ltd. (Yichang, China). 2, 20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), rutin, and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (St. Louis, MO, USA), while potassium sulfate was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Zinc sulfate, potassium ferrocyanide, absolute ethanol, petroleum ether, hydrochloric acid, sodium dihydrogen phosphate, and disodium hydrogen phosphate were acquired from Tianjin Damao Chemical Reagent Factory (Tianjin, China). All other chemicals were analytical grade.
+ Open protocol
+ Expand
3

Multicomponent Nanoformulation for Glioma Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glyceryl monostearate, medium chain triglycerides and poloxamer 188 were purchased from AIKE REAGENT Co., Ltd. (Chengdu, China). Absolute ethanol was purchased from the DAMAO Chemical Reagent Factory (Tianjin, China). Phosphoric acid and methanol were supplied by FUYU CHEMICAL (Tianjin, China). TMZ and CUR were purchased from Aladdin Co., Ltd. (Shanghai, China). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Med Chem Express Co., Ltd. (New Jersey, USA). Hoechst 33342 Live Cell Staining Solution was obtained from Beyotime Co., Ltd. (Shanghai, China). All other chemical compounds were of analytical purity. Rat glioma cell line (C6) and human brain glial normal cell line (HEB) were obtained from Jennio Biotech Co., Ltd. (Guangzhou, China).
+ Open protocol
+ Expand
4

Fabrication of Hydrophilic PTFE Film

Check if the same lab product or an alternative is used in the 5 most similar protocols
PTFE 0.1μm hydrophilic film (thickness 25~30 μm) was obtained from Asimo Technology Co., Ltd. (Shanghai, China). Dopamine hydrochloride (98%) was procured by Bomei Biotechnology Co., Ltd. (Hefei, China). Tris-HCl (99.5%) was obtained from Yami Biotechnology Co., Ltd. (Beijing, China). NaOH and absolute ethanol (AR) were acquired from Damao Chemical Reagent Factory (Tianjin, China). Ammonia (AR) and AgNO3 solution (0.5 mol/L) were supplied by Wengjiang Chemical Reagent Co., Ltd. (Shaoguan, China). Glucose (FG) was purchased from Weimeiyuan Food Ingredients Co., Ltd. (Shanghai, China). N-methylpyrrolidone (AR) was purchased from Yatai United Chemical Co., Ltd. (Wuxi, China). Ethylene glycol (AR) was obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Disodium ethylenediaminetetraacetic acid (EDTA) and NiCl2·6H2O (AR) were supplied by China National Pharmaceutical Group Chemical Reagent Co., Ltd. PVDF was purchased from Candlelight New Energy Technology Co., Ltd. (Dongguan, China).
+ Open protocol
+ Expand
5

Photopolymerizable Hydrogel Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tripropylene Glycol Diacrylate (TPGDA, 90% of the total of isomer, stabilized with MEHQ), urea, poly(vinyl alcohol) (PVA, Mw = 13,000–23,000), and dimethylaminobenzaldehyde (DMAB), were purchased from Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). The silicone oil (Dow Corning, viscosity~50 mPa·s) was obtained from CChip scientific instrument Co., Ltd. (Suzhou, China). Rhodamine B and 2-hydroxy-2-methylpropiophenone (HMPP) were obtained from Sigma-Aldrich Trading Co., Ltd. (Shanghai, China). Acrylic acid (AA) was purchased from Macklin Biochemical Co.,Ltd. (Shanghai, China). Phosphate buffer saline (PBS, 0.1 M) was obtained from Hewei Pharmaceutical Technology Co., Ltd. (Guangzhou, China). Absolute ethanol was obtained from Damao chemical reagent factory (Tianjin, China).
+ Open protocol
+ Expand
6

Kelp, Eggs, and Corn Oil Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fresh kelp (Laminaria japonica), fresh eggs (Charoen Pokphand Food, Charoen Pokphand Group), and corn oil (Fulinmen, China Oil and Foodstuffs Corporation) were obtained from a local supermarket in Guangzhou, Guangdong, China. Alkaline protease, glucose, and cholesterol standards were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). α-amylase, glucoamylase, and Rochelle salt were obtained from Sigma-Aldrich Trading Co., Ltd. (Shanghai, China). Cadmium (Ⅱ) chloride, copper (Ⅱ) sulfate, hydrochloric acid, sulfuric acid, glacial acetic acid, sodium hydroxide, and absolute ethanol were of analytical grade and purchased from Damao Chemical Reagent Factory (Tianjin, China).
+ Open protocol
+ Expand
7

Synthesis of Polysiloxane-based Materials

Check if the same lab product or an alternative is used in the 5 most similar protocols
The silicone resin (195T) whose main component is polysiloxane(SinOn−1H2n+2) was purchased from Shenzhen Ausbond Co., Ltd. (Shenzhen, China). The crucibles (60 × 30 × 15 mm) were purchased from Jiangsu Huida Medical Instruments Co., Ltd. (Yancheng, China). Absolute ethanol, acetone, petroleum ether, n-hexane and n-heptane were purchased from Tianjin Damao Chemical Reagent Factory. All reagents are analytical-grade and are used without further purification. The glass slides (7105, 25 × 75 × 1 mm) were purchased from Dalian Liaodong Chemical Reagent Factory.
+ Open protocol
+ Expand
8

Coal Sample Preparation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The coal sample used in the experiment was Hulunbuir lignite from Inner Mongolia. First, the lignite was collected using the quarter method. After being broken into smaller particles, the coal sample was crushed and collected with a small universal crusher. All coal samples were passed through a 100-mesh sieve (particle size = 0.147 mm) and then dried at 80 °C for 24 h to remove most of the water in the coal. After drying, the sample was placed into a dry bag followed by sealing and storing.
Concentrated sulfuric acid was purchased from Sinopharm Chemical Reagent Co., Ltd, China. Hydrogen peroxide (H2O2) was purchased from Shanghai Zhanyun Chemical Co., Ltd, China. Absolute ethanol was purchased from Damao Chemical Reagent Factory, China.
+ Open protocol
+ Expand
9

Synthesis and Characterization of Silver Nanowires

Check if the same lab product or an alternative is used in the 5 most similar protocols
The surface morphology of the silver nanowires was observed by field emission scanning electron microscopy (SEM, ULTRA 55, Zeiss, Oberkochen, Germany). The phase composition and structure were analyzed by X-ray diffraction (XRD, D8 Advance, Bruker, Berlin, Germany) at a scan rate of 2°/min with Cu Kα (λ = 1.54056 Å) radiation. Transmission electron microscopy (TEM, JEM-2100F, JEOL Ltd., Tokyo, Japan) was employed to analyze the crystal structure. The UV-visible spectrophotometer (UV-2550, Shimadzu Corporation, Tokyo, Japan) was used to characterize the UV-visible absorption spectrum of silver nanowires and the optical transmittance of transparent conductive films. The sheet resistance of the conductive films were carried out by a four-point probe method (KDB-1, Guangzhou Kunde Technology Co., Ltd., Guangzhou, China). Silver nitrate (AgNO3, 98%) was acquired from Sinopharm Chemical Reagent Co., Ltd., sodium chloride (NaCl, 99.9%) was obtained from Sigma–Aldrich (St. Louis, MO, USA)., sodium bromide (NaBr, 99.0%) was provided by Fuchen Chemical Reagent Factory (Tianjin, China), polyvinylpyrrolidone (PVP, MW ≈ 1,300,000) was supplied by Macklin Biochemical Co., Ltd. (Shanghai, China), ethylene glycol (EG, 99.8%), and absolute ethanol (99.7%) were acquired from Damao Chemical Reagent Factory (Tianjin, China).
+ Open protocol
+ Expand
10

Evaluation of Antioxidant Capacity in RIN-m5F Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
RIN-m5F (ATCC CRL-11605) cells were purchased from the Tongpai Biotechnology Co. Ltd., Shanghai, China. Cytiva (Uppsala, Sweden) provided diethylaminoethyl 52 (DEAE-52) cellulose and Sephadex G-100. Sodium chloride, anthrone, D-glucose, penicillin-streptomycin solution (100×, including penicillin 10 ku/mL and streptomycin 10 mg/mL), phosphate buffer solution (1×, pH 7.2–7.4, 0.01 M), trypsin-EDTA solution, and Total Antioxidant Capacity (T-AOC) Assay Kit were purchased from Beijing Solarbio Science and Tech. Co. (Beijing, China). Gibco RPMI medium 1640 basic (1×) was arranged from ThermoFisher Biochemical Products (Beijing) Co. (Beijing, China). Fetal bovine serum (FBS, Australia Origin) and Cell Counting Kit-8 were arranged by Invigentech Inc. (Irvine, CA, USA). Sigma-Aldrich Co. (St. Louis, MO, USA) provided the 2-diphenyl-1-picrylhydrazyl (DPPH). Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu, China) provided the Hydroxyl Free Radical Assay Kit. Absolute ethanol, chloroform, n-butanol, concentrated sulfuric acid, and potassium bromide were purchased from the Tianjin Damao Chemical Reagent Co. (Tianjin, China). All compounds were analytical grade unless otherwise specified.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!