Aquamax ultra water purification system

HA (MW = 2.34 × 10⁵ g/mol) was purchased from Lifecore Biomedical Inc. (Chaska, MN, USA). 5β-cholanic acid (CA), DOX·HCl, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), formamide, dimethylformamide (DMF), TA, and iron (III) chloride hexahydrate (FeCl₃·6H₂O) were purchased from Sigma Aldrich Co. (St. Louis, MO, USA) and were used as received. RPMI medium and fetal bovine serum (FBS) were purchased from Capricorn Scientific GmbH (Ebsdorfergrund, Germany). Dulbecco’s Phosphate-Buffered Saline (DPBS) was purchased from WELGENE Inc. (Kyungsan, Korea) for in vitro experiments. The water used in this study was purified by using the Young Lin aquaMAX-ultra water purification system (Anyang, Korea).

HA sodium salt (molecular weight = 10–12 kDa) was purchased from Lifecore Biomedical (MN, USA). Methoxy poly(ethylene glycol) amine (PEG‐NH₂, molecular weight = 5 kDa) was obtained from Laysan Bio Inc. (AL, USA). Cy5.5‐DBCO and Ac₄ManNAz were purchased from Click Chemistry Tools (AZ, USA). Thiazolyl blue tetrazolium bromide (MTT), DBCO‐amine and dimethylformamide (DMF) were obtained from Sigma‐Aldrich Co. (MO, USA). Sodium cyanoborohydride (NaBH₃CN) was purchased from TCI (Tokyo, Japan). Poly(vinylidene fluoride) membrane was obtained from Millipore (MA, USA). Cy5.5 NHS ester dye was purchased from GE Healthcare (IL, USA). Foetal bovine serum (FBS), RPMI 1640, Dulbecco's phosphate‐buffered saline (DPBS), antibiotic‐antimycotic (AA) solution (100×) and trypsin‐trypsin‐ethylene diamine tetraacetic acid were purchased from WelGENE (Gyeongsan, Korea). NIH/3T3, CT26, RAW264.7, MDA‐MB‐231 and HCT‐116 were provided by the Korean Cell Line Bank (Seoul, Korea). Water was purified using an AquaMax‐Ultra water purification system from Younglin Co. (Anyang, Korea). All other chemicals were of analytical grade and used without further purification. Information on the antibodies used in the experiments is listed in Table S1.