The protein samples were extracted from the frozen livers according to previous protocols37 (link). The samples dissolved in the Yeasen Biotech sodium dodecyl sulphate (SDS)-loading buffer (Shanghai, China) were electrophoresed and transferred to Merck Millipore polyvinylidene difluoride (PVDF) membranes (Billerica, MA, USA) and then subjected to immunoblot analysis with the antibodies against p-JNK, JNK, p-cPLA2, cPLA2, platelet-type 12-LOX and leukocyte-type 12-LOX. The images were captured by the CLINX ChemiScope 3300 mini system (Shanghai, China). The relative protein abundance in each group was quantified by National Institute of Health Image J software (Silver Springs, MD, USA).
Chemiscope 3300 mini system
Manufactured by Clinx
Sourced in China
The ChemiScope 3300 mini system is a compact and versatile laboratory equipment designed for various analytical applications. It provides users with a reliable and efficient platform for conducting essential analyses and experiments. The core function of the ChemiScope 3300 mini system is to facilitate the collection and analysis of data, serving as a valuable tool for researchers and scientists in their respective fields of study.
Automatically generated - may contain errors
2 protocols using chemiscope 3300 mini system
1
Western Blot Analysis of Liver Proteins
2
Western Blot Analysis of TIMM50 Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tumour and adjacent tissue samples were lysed completely in extraction buffer. The supernatants were centrifuged and collected. The protein concentrations were determined according to the BCA protocol. Equal amounts of protein samples (30 µg) were separated by 10% SDS-PAGE. Then, the proteins were transferred onto polyvinylidene fluoride (PVDF) membranes. Next, the PVDF membranes were incubated with primary antibodies at 4°C overnight, including TIMM50 and GAPDH (1 : 1000, Santa Cruz Biotechnology Inc., Santa Cruz, USA). Then, the membranes were incubated at room temperature with HRP-conjugated secondary antibodies (1 : 10,000 dilution) for 2 h. The results were obtained using an ECL substrate (Beyotime Biotechnology, Beyotime, China) detected by a ChemiScope 3300 Mini system (Clinx Science Instruments Co. Ltd., Clinx, Shanghai, China). GAPDH acted as a loading control.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!