Human prostate normal cell line (RWPE-1) and human prostate cancer cell lines; LNCaP and PC-3, were purchased from the American Type Culture Collection (ATCC, Manassas, Virginia, USA). LNCaP and PC-3 cells were grown in Roswell Park Memorial Institute medium (RPMI) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO), 1% penicillin and streptomycin. RWPE-1 cells were maintained in Keratinocyte Serum Free Medium (K-SFM, ATCC) supplemented with bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). Mediums were supplemented with 10% heat-inactivated fetal calf serum (Sigma.), 100 U/ml penicillin and 100 mg/ml streptomycin (Flowlab, Sydney, Australia). All cells were maintained in a humidified atmosphere of 5% CO2 in air at 37°C incubator.
K sfm
Manufactured by American Type Culture Collection
Sourced in United States
K-SFM is a serum-free, low-calcium medium designed for the culture of epithelial cells. It contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, and growth factors required for the cultivation of epithelial cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Fetal calf serum (fcs), by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Penicillin, by Merck Group (1 mentions)
Rwpe 1, by American Type Culture Collection (1 mentions)
Lncap, by Merck Group (1 mentions)
Crl 2190, by American Type Culture Collection (1 mentions)
2 protocols using k sfm
1
Prostate Cell Line Culture Conditions
2
Wogonin Mitigates High Glucose-Induced Cytotoxicity
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Human renal proximal tubule HK-2 cells (CRL-2190™, ATCC, VA, USA) were maintained in keratinocyte serum-free medium (K-SFM, 17005-042, ATCC, VA, USA) in a cell culture incubator. HK-2 cells were subject to 30 mmol/L of glucose for 24 h as high glucose exposure (Cui et al. 2023; (link)Li et al. 2021; (link)Liu et al. 2023) (link). To investigate the roles of wogonin in HG-induced cell toxicity, we exposed HK-2 cells to increasing concentrations of wogonin (1, 2, 4, 8, 16, 32, and 64 µM) for 24 h to determine the suitable concentration for acute toxicity. We chose 8 µM as an optimal concentration for the co-treatment with wogonin and HG based on the cell viability. The treatment with mannitol (8 µM) for 24 h was used as the negative control. Mannitol plus HG medium was used as a control. In brief, cells were divided into three groups: (1) negative control, (2) HG, and (3) HG + wogonin. For in vitro cell viability, the assays were conducted using a commercial 992, Beijing, China) .
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