Elia calprotectin

Manufactured by Thermo Fisher Scientific

Sourced in Germany, Sweden

The EliA Calprotectin is a laboratory test used to measure the levels of calprotectin, a protein found in white blood cells. It is primarily used as a biomarker to aid in the assessment and monitoring of inflammatory bowel diseases, such as Crohn's disease and ulcerative colitis.

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Lab products found in correlation

Fecal sample preparation kit, by Roche (2 mentions) Phadia 100, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

EliA Calprotectin 2 assay EliA® Calprotectin 2 Calprotectin

4 protocols using elia calprotectin

Calprotectin Measurement from Stool Samples

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Stool samples were collected the day before beginning bowel preparation
(preferably from the first stool in the morning) and then kept in the fridge
until they were brought to the hospital. Within a maximum of 7 days after
collection, stools were extracted in accordance with the manufacturer’s
instructions, using a fecal sample preparation kit (Roche Diagnostics, Mannheim,
Germany). Sample extracts were stored at -80ºC until the assays were performed
at the Department of Pharmacology and Therapeutics, Faculty of Medicine of the
University of Porto. Samples were thawed and analyzed using a commercially
available fluoroenzyme immunoassay (EliA Calprotectin^®, ThermoFisher
Scientific, Freiburg, Germany).

Lopes S., Andrade P., Afonso J., Cunha R., Rodrigues-Pinto E., Ramos I., Macedo G, & Magro F. (2018). Monitoring Crohn’s disease activity: endoscopy, fecal markers and computed tomography enterography. Therapeutic Advances in Gastroenterology, 11, 1756284818769075.

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Fecal Calprotectin Quantification Protocol

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Stool samples were collected and kept at 4 °C (for a maximum of 48 h) until shipment to the central laboratory (Department of Pharmacology and Therapeutics, Faculty of Medicine of University of Porto). FC was extracted from stools within a maximum of seven days after collection using the ‘Fecal sample preparation kit’ (Roche Diagnostics, Germany) according to the instructions provided by the manufacturer and stored at − 80 °C until quantification. FC samples were quantified using a fluoroenzyme immunoassay (EliA Calprotectin®, Thermo Fisher Scientific, Germany) according with manufacturers' instructions.

Magro F., Afonso J., Lopes S., Coelho R., Gonçalves R., Caldeira P., Lago P., de Sousa H.T., Ramos J., Gonçalves A.R., Ministro P., Rosa I., Vieira A.I., Andrade P., Soares J.B., Carvalho D., Sousa P., Meira T., Lopes J., Moleiro J., Dias C.C., Falcão A., Geboes K, & Carneiro F. (2017). Calprotectin and the Magnitude of Antibodies to Infliximab in Clinically-stable Ulcerative Colitis Patients are More Relevant Than Infliximab Trough Levels and Pharmacokinetics for Therapeutic Escalation. EBioMedicine, 21, 123-130.

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Stool Calprotectin and FIT Analysis

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The first stool sample produced in the hospital following polyethylene glycol administration for colonoscopy preparation was collected. After defecating on a dedicated sheet on the stool seat, the patients collected a small piece of the stool sample in a container. Both calprotectin and FIT were measured in the stool samples. FC was measured with a fluorescence enzyme immunoassay (Elia Calprotectin [Thermo Fisher, Uppsala, Sweden]), and FIT was performed using OC‐Sensor PLEDIA (Eiken Chemical, Tokyo, Japan).

Sakurai T., Akita Y., Miyashita H., Miyazaki R., Maruyama Y., Saito T., Shimada M., Yamasaki T., Arhihiro S., Kato T., Matsuura T., Ikegami M., Okayasu I, & Saruta M. (2022). Prostaglandin E‐major urinary metabolite diagnoses mucosal healing in patients with ulcerative colitis in remission phase. Journal of Gastroenterology and Hepatology, 37(5), 847-854.

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Measurement of Fecal Calprotectin Levels

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Fecal calprotectin levels were measured at the University Hospital Zurich. Specimens from other study centers were sent to the laboratory by mail. The Calprotectin test is called EliA Calprotectin (Thermo Fisher Scientific, Uppsala, Sweden) and uses the FEIA method (fluorescence enzyme immunoassay) on a fully automated system called Phadia 100 (Thermo Fisher Scientific, Uppsala, Sweden). The EliA Calprotectin Wells are coated with monoclonal antibodies to calprotectin. If present in the patient’s specimen, calprotectin binds to the coated antibodies. After washing away non-bound components, enzyme-labeled antibodies against human calprotectin (EliACalprotectin Conjugate) are added to form a calprotectin-conjugate complex. After incubation, non-bound conjugate is washed away and the bound complex is incubated with a Development Solution. After stopping the reaction, the fluorescence in the reaction mixture is measured. The higher the response value, the more calprotectin is present in the specimen. To evaluate test results, the response for patient samples is compared directly to the response for calibrators.

Chmiel C., Senn O., Hasler S., Rosemann T., Rogler G., Zahnd N., Tandjung R., Scherz N., Sulz M.C, & Vavricka S. (2019). Can the CalproQuest predict a positive Calprotectin test? A prospective diagnostic study. PLoS ONE, 14(11), e0224961.

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