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Ab4047

Manufactured by Abcam
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Sourced in United Kingdom

Ab4047 is a primary antibody product offered by Abcam. It is a recombinant monoclonal antibody targeting a specific protein. The antibody is produced in a mammalian expression system and is purified using protein A affinity chromatography.

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Lab products found in correlation

A5441, by Merck Group (3 mentions) Alexa fluor conjugated secondary antibody, by Jackson ImmunoResearch (2 mentions) Ab166910, by Abcam (2 mentions) Ab92544, by Abcam (2 mentions) Ab36969, by Abcam (2 mentions) Ab18180, by Abcam (2 mentions) Mab645, by R&D Systems (2 mentions) Nbp1 04278, by Novus Biologicals (2 mentions) Ab8245, by Bio-Rad (2 mentions) Goat anti rat igg horseradish peroxidase conjugated haf005, by R&D Systems (2 mentions) Nb400 131, by Novus Biologicals (2 mentions) Goat anti mouse igg h l hrp conjugate, by Bio-Rad (2 mentions) Pvdf membrane, by Avantor (1 mentions) A2066, by Merck Group (1 mentions) Ab34173, by Abcam (1 mentions) Cocktails of protease, by Thermo Fisher Scientific (1 mentions) Hrp conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg horseradish peroxidase conjugate, by Bio-Rad (1 mentions) Goat anti mouse igg horseradish peroxidase conjugate, by Bio-Rad (1 mentions)

6 protocols using ab4047

1

Western Blotting Protein Detection Protocol

Cited 1 time
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Western blots were performed as previously described (49 (link)). In brief, protein lysates from cell culture or tumor tissues were extracted in ice-cold RIPA buffer (50 mM Tris-Cl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) containing cocktails of protease (Thermo Scientific) and phosphatase inhibitors (Sigma-Aldrich), by centrifugation (13000g, 15 min) at 4°C following 3 freeze-thaw cycles. Proteins were separated by SDS-PAGE and transferred to methanol activated polyvinylidene difluoride (PVDF) membrane (VWR). The primary antibodies used include the following: rabbit anti-mouse Fbln5 (1:1000), rabbit anti-human Fbln5 (1:500) (HPA000868, Sigma Aldrich), anti-Nqo1 (1:1000) (ab34173, Abcam), anti-α-Tubulin (1:1000) (ab4047, Abcam) and anti-β-actin (1:5000) (A2066, Sigma-Aldrich). HRP-conjugated donkey anti-rabbit IgG (1:10000) (Jackson Immunoresearch) as secondary antibodies were used.
Wang M., Topalovski M., Toombs J.E., Wright C.M., Moore Z.R., Boothman D.A., Yanagisawa H., Wang H., Witkiewicz A., Castrillon D.H, & Brekken R.A. (2015). Fibulin-5 blocks microenvironmental ROS in pancreatic cancer. Cancer research, 75(23), 5058-5069.
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2

Western Blot Analysis of DKK1 in Aged Rats

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Protein from aged rat lysates ± RLX (n = 3/group) was separated using pre-cast Mini-PROTEAN TGX 7.5% polyacrylamide gels and transferred to a PVDF membrane. Membranes were then probed for mouse anti-DKK1 (1:500, Santa Cruz sc374574) and rabbit anti-α-tubulin (1:1000, Abcam, ab4047). Membranes were developed using Pierce ECL Western Blotting Substrate (ThermoScientific, #32109) according to manufacturer instructions and developed by autoradiography. Quantification of the optical density of DKK1 or tubulin bands were performed using ImageJ software and DKK1 expression was normalized to α-tubulin.
Martin B., Gabris B., Barakat A.F., Henry B.L., Giannini M., Reddy R.P., Wang X., Romero G, & Salama G. (2019). Relaxin reverses maladaptive remodeling of the aged heart through Wnt-signaling. Scientific Reports, 9, 18545.
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3

Immunoblotting and Immunofluorescence Antibodies

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In the experimental procedures described, the following antibodies were used: rabbit monoclonal anti-LRP1 (Ab92544; Abcam), mouse monoclonal anti-ABCA1 (Ab18180; Abcam), rabbit monoclonal anti-PSAP (ab166910; Abcam), mouse monoclonal anti-CTSD (NBP1-04278 Novus Biologicals), goat anti–SR-B1 (NB400-131; Novus Biologicals), rabbit anti-ABCG1 (ab36969; Abcam), rat anti–Wnt-5a (MAB645; R&D Systems), mouse monoclonal anti–β-actin (A5441, Sigma-Aldrich, 1:5,000), anti–α-tubulin (Ab4047, Abcam), anti-GAPDH (Ab8245), rabbit anti-GST (sc-459), goat anti-rabbit IgG-horseradish peroxidase conjugate (170–6515: Bio-Rad Laboratories,), goat anti-mouse IgG-horseradish peroxidase conjugate (170-6516; Bio-Rad Laboratories), and goat anti-Rat IgG-horseradish peroxidase conjugated (HAF005; R&D Systems). For immunofluorescence, Alexa Fluor–conjugated secondary antibodies and Alexa Fluor 488–conjugated and 568–conjugated antibodies were purchased from Jackson ImmunoResearch.
Oldoni F., van Capelleveen J.C., Dalila N., Wolters J.C., Heeren J., Sinke R.J., Hui D.Y., Dallinga-Thie G.M., Frikke-Schmidt R., Hovingh K.G., van de Sluis B., Tybjærg-Hansen A, & Kuivenhoven J.A. (2018). Naturally Occurring Variants in LRP1 (Low-Density Lipoprotein Receptor–Related Protein 1) Affect HDL (High-Density Lipoprotein) Metabolism Through ABCA1 (ATP-Binding Cassette A1) and SR-B1 (Scavenger Receptor Class B Type 1) in Humans. Arteriosclerosis, Thrombosis, and Vascular Biology, 38(7), 1440-1453.
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4

Comprehensive Antibody Profiling for Cellular Analysis

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In these experimental procedures we used the following antibodies: rabbit polyclonal antibody against COMMD1 (11938-1-AP, Proteintech Group, USA), mouse anti-β-Actin (A5441, Sigma-Aldrich Chemie B.V., Zwijndrecht, the Netherlands), rabbit anti-Tubulin (AB4047, Abcam, Cambridge, UK), rabbit anti-Lamin A/C (2032, Cell Signaling Technology Europe, B.V., Leiden, the Netherlands), rabbit anti-p65 (4764, Cell Signaling Technology, Europe, B.V.), rabbit anti-IκBα (sc-371, Santa Cruz Biotechnology Inc., Heidelberg, Germany), rabbit anti-Cd68 (#137002, Biolegio, Nijmegen, the Netherlands) rabbit anti-F4/80 (#101201, Biolegio, Nijmegen, the Netherlands), goat anti-rabbit IgG (H + L)-HRP Conjugate (170-6515, Bio-Rad Laboratories BV, Veenendaal, the Netherlands), goat anti-mouse IgG (H + L)-HRP Conjugate (170-6516, Bio-Rad Laboratories BV).
Bartuzi P., Wijshake T., Dekker D.C., Fedoseienko A., Kloosterhuis N.J., Youssef S.A., Li H., Shiri-Sverdlov R., Kuivenhoven J.A., de Bruin A., Burstein E., Hofker M.H, & van de Sluis B. (2014). A cell-type-specific role for murine Commd1 in liver inflammation. Biochimica et biophysica acta, 1842(11), 2257-2265.
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5

Antibody Characterization Protocol

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In the experimental procedures described the following antibodies were used: rabbit monoclonal anti-LRP1 (Ab92544; Abcam), mouse monoclonal anti-ABCA1 (Ab18180; Abcam), rabbit monoclonal anti-PSAP (ab166910; Abcam), mouse monoclonal anti-Cathepsin D (NBP1-04278 Novus Biologicals), goat anti-SR-B1 (NB400-131; Novus Biologicals), rabbit anti-ABCG1(ab36969; Abcam), rat anti-Wnt-5a (MAB645; R&D Systems), mouse monoclonal anti-β actin (A5441, Sigma-Aldrich, 1:5,000), anti-α tubulin (Ab4047, Abcam), anti-GAPDH (Ab8245), rabbit anti-GST (sc-459), goat anti-rabbit IgG-horseradish peroxidase (HRP) conjugate (170-6515: Bio-Rad Laboratories,), goat anti-mouse IgG-HRP conjugate (170-6516; Bio-Rad Laboratories), goat anti-Rat IgG Horseradish Peroxidase conjugated (HAF005; R&D Systems). For immunofluorescence, Alexa Fluor–conjugated secondary antibodies and Alexa Fluor 488 and 568–conjugated were purchased from Jackson ImmunoResearch.
Oldoni F., van Capelleveen J.C., Dalila N., Wolters J.C., Heeren J., Sinke R.J., Hui D.Y., Dallinga-Thie G.M., Frikke-Schmidt R., Hovingh K.G., van de Sluis B., Tybjærg-Hansen A, & Kuivenhoven J.A. (2018). Naturally Occurring Variants in LRP1 (Low-Density Lipoprotein Receptor–Related Protein 1) Affect HDL (High-Density Lipoprotein) Metabolism Through ABCA1 (ATP-Binding Cassette A1) and SR-B1 (Scavenger Receptor Class B Type 1) in Humans. Arteriosclerosis, Thrombosis, and Vascular Biology, 38(7), 1440-1453.
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6

COMMD1 and LDLR Protein Detection Protocol

Cited 2 times
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In the experimental procedures described, the following antibodies were used: rabbit polyclonal antibody against COMMD1 (11938-1-AP, Proteintech Group, 1:1,000); rabbit polyclonal antibody against LDLR (PAB8804, Abnova, 1:1,000); rabbit polyclonal antibody against GST (Z-5) (sc-459, Santa Cruz Biotechnology, 1:5,000); goat anti-rabbit IgG (H+L)-horseradish peroxidase (HRP) conjugate (170-6515, Bio-Rad Laboratories, 1:10,000); goat anti-mouse IgG (H+L)-HRP conjugate (170-6516, Bio-Rad Laboratories, 1:10,000); mouse anti-β-actin (A5441, Sigma-Aldrich, 1:5,000); rabbit anti-tubulin (AB4047, Abcam, 1:2,000); rabbit anti-Rab11 (700184, Invitrogen, 1:1,000); rabbit anti-EEA-1 (AB2900, Abcam, 1:1,000); rabbit anti-CCDC22 (16636-1-AP, Proteintech, 1:2,000); and rabbit anti-CCDC93 (20861-1-AP, Proteintech, 1:5,000). Rabbit polyclonal antibodies against WASH1, FAM21 and VPS35 were previously described1 (link)5 (link). Anti-apoB100 (1:1,000) and rabbit anti-apoA1 (1:1,000) antibodies were a gift from A.K. Groen. Rabbit polyclonal antibody against ARH (1:1,000) was a gift from H.H. Hobbs. Densitometry analysis of western blot bands was performed using Image Lab 3.0.1 software (Bio-Rad Laboratories). Images have been cropped for presentation. Full-size images are presented in Supplementary Fig. 12.
Bartuzi P., Billadeau D.D., Favier R., Rong S., Dekker D., Fedoseienko A., Fieten H., Wijers M., Levels J.H., Huijkman N., Kloosterhuis N., van der Molen H., Brufau G., Groen A.K., Elliott A.M., Kuivenhoven J.A., Plecko B., Grangl G., McGaughran J., Horton J.D., Burstein E., Hofker M.H, & van de Sluis B. (2016). CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL. Nature Communications, 7, 10961.
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