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500 g genomic tips

Manufactured by Qiagen
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Sourced in Germany

The 500/G genomic tips are a specialized laboratory equipment designed for the efficient extraction and purification of genomic DNA from various sample types. The core function of these tips is to provide a reliable and consistent method for DNA isolation, which is a crucial step in many genomic and molecular biology applications.

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Lab products found in correlation

Genomic dna buffer, by Qiagen (1 mentions) Genomic dna buffer set, by Qiagen (1 mentions)

Close spelling variants of this product

Genomic-tip 500/G Genomic-tip 500/G kit QIAGEN genomic tip columns (500/G) Genomic-tip QIAGEN-tip 500

2 protocols using 500 g genomic tips

1

Genomic DNA Extraction and Illumina Sequencing

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Genomic DNA was isolated from a pure culture of SG61-1L with Qiagen Genomic DNA buffers and 500/G genomic tips (Qiagen, Hilden, Germany). Short (500-bp)- and long (2,000-bp)-insert libraries were sequenced by MiSeq Illumina technology at the Micromon DNA sequencing facility (Monash University, Melbourne, Australia). The raw reads were then assembled with the SPAdes assembler version 3.5.0 (42 (link)), which performs error correction, assembly, scaffolding, and mismatch correction. The final assembly was 3,771,711 bp long with a GC content of 63% and was composed of three scaffolds 3.6 Mb, 94 kb, and 10 kb in length. Annotation of this assembly was performed by the NCBI Prokaryotic Genome Annotation Pipeline and identified 3,209 protein-coding sequences, 49 tRNA sequences, and two rRNA clusters.
Palamuru S., Dellas N., Pearce S.L., Warden A.C., Oakeshott J.G, & Pandey G. (2015). Phylogenetic and Kinetic Characterization of a Suite of Dehydrogenases from a Newly Isolated Bacterium, Strain SG61-1L, That Catalyze the Turnover of Guaiacylglycerol-β-Guaiacyl Ether Stereoisomers. Applied and Environmental Microbiology, 81(23), 8164-8176.
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2

Bacterial Genomic DNA Extraction

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Genomic DNA was isolated from 10 ml of LB-grown bacterial overnight cultures using a Qiagen genomic DNA buffer set combined with Qiagen 500/G Genomic-tips. The extraction was performed according to the manufacturer’s protocol.
Flaugnatti N., Isaac S., Lemos Rocha L.F., Stutzmann S., Rendueles O., Stoudmann C., Vesel N., Garcia-Garcera M., Buffet A., Sana T.G., Rocha E.P, & Blokesch M. (2021). Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms. Nature Communications, 12, 5751.
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