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Cary 300 uv visabsorption spectrophotometer

Manufactured by Agilent Technologies

The Cary 300 UV–vis absorption spectrophotometer is a laboratory instrument designed to measure the absorbance of light by a sample across the ultraviolet and visible wavelength ranges. It provides quantitative analysis capabilities for a variety of applications.

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3 protocols using cary 300 uv visabsorption spectrophotometer

1

Spectroscopic Characterization of NCs

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The UV–visible
absorption spectra were recorded using a Varian Cary 300 UV–vis
absorption spectrophotometer. The PL spectra were measured on a Varian
Cary Eclipse spectrophotometer using an excitation wavelength (λex) of 350 nm for all of the samples. Samples were prepared
by diluting NC solutions in toluene, in quartz cuvettes with a path
length of 1 cm.
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2

Optical Absorption and Photoluminescence Characterization

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Optical absorption
spectra were collected on a Varian Cary 300 UV–vis absorption
spectrophotometer, while PL spectra were recorded by a Varian Cary
Eclipse spectrophotometer using an excitation wavelength at 370 nm
for all of the samples. The NC solutions were diluted in toluene in
quartz cuvettes (path length = 1 cm) to a maximum optical density
below 1.0, to minimize self-absorption effects. The time-resolved
PL measurements were performed on an Edinburgh FLS900 fluorescence
spectrometer equipped with a time-correlated single photon counting
unit coupled with an Edinburgh Instruments EPL-510 pulsed laser diodes
exc = 372 nm, pulse width = 68.8 ps, and λexc = 508.2 nm, pulse width = 177.0 ps). Data analysis was
carried out using DecayFit software.
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3

Optical Characterization of Nanocrystals

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The UV–vis
absorption spectra were recorded using a Varian Cary 300 UV–vis
absorption spectrophotometer. The PL spectra were measured on a Varian
Cary Eclipse spectrophotometer using an excitation wavelength (λex) of 350 nm for all of the chloride and bromide samples
and 450 nm for all of the iodide compounds. Samples were prepared
by diluting NC solutions in toluene in quartz cuvettes with a path
length of 1 cm.
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