Anti cd49b dx5

Manufactured by BioLegend

Sourced in United States

Anti-CD49b (DX5) is a mouse monoclonal antibody that recognizes the CD49b (Alpha-2 Integrin) cell surface antigen. CD49b is expressed on natural killer (NK) cells, a subset of T cells, and platelets.

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Lab products found in correlation

Anti cd16 cd32 antibody, by Thermo Fisher Scientific (1 mentions) Foxp3 transcription factor staining buffer kit, by Thermo Fisher Scientific (1 mentions) Anti t bet 4b10, by Thermo Fisher Scientific (1 mentions) Anti foxo1 c29h4, by Cell Signaling Technology (1 mentions) Recombinant il 33, by Thermo Fisher Scientific (1 mentions) Anti nk1.1 pk136, by Thermo Fisher Scientific (1 mentions) Recombinant il 7, by Thermo Fisher Scientific (1 mentions) D4902, by Merck Group (1 mentions) Streptavidin microbeads, by Miltenyi Biotec (1 mentions) Fortessa, by BD (1 mentions) Anti cd4 gk1, by BioLegend (1 mentions) Anti f4 80 bm8, by BioLegend (1 mentions) Anti cd8α 53 6.7, by BioLegend (1 mentions) Facs lysing solution, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Anti cd3 17a2, by BioLegend (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by BioLegend (1 mentions) Anti cd45 30 f11, by BioLegend (1 mentions) Rat anti mouse cd16 cd32, by BD (1 mentions) Anti cd11c n418, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-CD49b (9 citations) CD49b (DX5; (5 citations) Anti-CD49b (clone DX5, (4 citations) Biotin anti-CD49b (DX5) (2 citations)

4 protocols using anti cd49b dx5

Multiparametric Flow Cytometry for Immune Cell Analysis

Cited 2 times

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Flow cytometry was performed as described previously (11 (link), 14 (link)). In details, single cells were isolated from mouse BM, spleen, and periphery lymph nodes (pLN). If necessary, BM cells were counted by Fuchs-Rosenthal Counting Chamber. Briefly, to analyze the surface marker, cells were blocked with anti-CD16/CD32 antibodies (eBioscience, San Diego, CA) and then stained with surface marker antibodies and washed by PBS. To analyze the intracellular proteins, after staining with surface markers, cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit following the manufacturer's protocols (eBioscience, San Diego, CA).
Antibodies used were as followings: anti-Foxo1 (C29H4) was from Cell Signaling Technology (Boston, MA); anti-NKp46 (29A1.4), anti-CD19 (6D5), anti-CD3 (17A2), anti-Gr1 (RB6-8C5), anti-Ter119 (Ter119), anti-CD4 (GK1.5), anti-CD8 (53-6.7), anti-c-Kit (2B8), anti-Sca-1 (D7), anti-CD127 (A7R34), and anti-CD49b (DX5) were from Biolegend (San Diego, CA); anti-CD27 (LG.3A10), anti-CD11b (M1/70), anti-B220 (RA3-6B2), and anti-CD122 (TM-β1) were form BD Biosciences (San Diego, CA); anti-CD43 (S7), anti-KLRG1 (2F1), anti-CD135(A2F10), anti-Ki-67 (SolA15), and anti-T-bet (4B10) were from eBioscience (San Diego, CA).

Huang P., Wang F., Yang Y., Lai W., Meng M., Wu S., Peng H., Wang L., Zhan R., Imani S., Yu J., Chen B., Li X, & Deng Y. (2019). Hematopoietic-Specific Deletion of Foxo1 Promotes NK Cell Specification and Proliferation. Frontiers in Immunology, 10, 1016.

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Isolation and Activation of Murine ILC2s

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Ten- to 12-week-old B10.BR and BALB/c mice were given 0.4 µg of recombinant mouse IL-17E/IL-25 (R&D Systems, Minneapolis, MN) for 4 days. On day 5, cells were isolated from mesenteric lymph nodes and peritoneum by peritoneal lavage using RPMI with 10% fetal bovine serum (complete media). ILC2s were isolated by negative selection with a MACs column using biotinylated antibodies [anti-CD8α (53-6.7), anti-CD4 (RM 4.4), anti-CD3ε (145-2C11), anti-γδTCR (UC7-13DS), anti-TER119 (TER-119), anti-B220 (RA3-6B2), anti-CD11b (M1/70), and anti-NK1.1 (PK136) (all; eBioscience, San Diego, CA); anti-CD11c (N418), anti-CD19 (MB19-1), anti-Ly6G (1A8), and anti-CD49b (DX5) (all; BioLegend, San Diego, CA); and Streptavidin Microbeads (130-048-101; Miltenyi Biotec, Cambridge, MA)]. Cells were cultured for 6 days in complete media with recombinant IL-7 and recombinant IL-33 (10 ng/mL) (PeproTech, Rocky Hill, NJ), changing the media every 2 days. ILC2 activation was evaluated by using flow cytometry on day 6 by intracellular cytokine staining of lineage-specific markers, ST2, KLRG1, and expression of IL-13. For steroid response assays, dexamethasone (10 nM) (D4902; MilliporeSigma, Burlington, MA) was added to ILC2 cultures on day 4 and evaluated 48 hours later.

Bruce D.W., Kolupaev O., Laurie S.J., Bommiasamy H., Stefanski H., Blazar B.R., Coghill J.M, & Serody J.S. (2021). Third-party type 2 innate lymphoid cells prevent and treat GI tract GvHD. Blood Advances, 5(22), 4578-4589.

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Flow Cytometry Analysis of B Cells

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Flow cytometry was performed with an LSRII or Fortessa (BD) and analyzed with Flowjo (Tree Star, Ashland, OR). All antibodies were purchased from Ebioscience, unless specified. For I-E^d-specific B cell staining, cells were preblocked at 4°C with 2.4G2, followed by incubation for 30 mins with lineage-specific antibodies (for dump channel: Anti-CD3 (17A2), anti-CD4 (GK1.5), anti-CD8α (53-6.7), anti-F4/80 (BM8, Biolegend), and anti-CD49b (DX5, Biolegend)), anti-B220 (RA3-6B.2), anti-IgD (11-26C.2a), anti-Fas (JO2, BD Pharmingen), and anti-GL7 (GL7), and followed by I-E^d tetramers.

Yang J., Chen J., Young J., Wang Q., Yin D., Sciammas R, & Chong A.S. (2016). Tracing donor-MHC Class II reactive B cells in mouse cardiac transplantation: delayed CTLA4-Ig treatment prevents memory alloreactive B cell generation. Transplantation, 100(8), 1683-1691.

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Comprehensive Immune Cell Profiling of BAL and Lung

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To analyze immune cell populations, BAL and lung cells were incubated with rat anti-mouse CD16/CD32 (BD Biosciences) blocking antibody for 10 min at RT. Then, the BAL cells were stained with anti-Gr-1 (RB6-8C5, BioLegend, San Diego, CA, United States), anti-Siglec-F (E50-2440, BD Biosciences), Ly6c (AL-21, BD Biosciences), anti-MHCII (M5/114.15.2, eBioscience, San Diego, CA, United States), anti-CD11b (M1-70, BioLegend), anti-CD14 (Sa14-2, BioLegend), anti-CD11c (N418, eBioscience), anti-CD49b (DX5, BioLegend), anti-PDCA-1 (eBio927, eBioscience), DAPI (BioLegend), and anti-CD45 (30-F11, BioLegend) antibodies for 30 min at 4°C in dark. The lung cells were also stained with anti-CD3 (17A2, BioLegend), anti-CD4 (RM4-5, BioLegend), anti-CD8 (53-6.7, BioLegend), and anti-CD45 (30-F11, BioLegend) antibodies for 30 min at 4°C in a dark place. The stained cells were fixed in FACS lysing solution (BD biosciences) and analyzed by BD LSR Fortessa (BD Biosciences). All flow cytometry data were analyzed by Flowjo software (TreeStar Inc., Ashland, OR, United States). The gating strategy for immune cell populations is described in Supplementary Figures S1, S2.

Lee Y.J., Lee J.Y., Jang Y.H., Seo S.U., Chang J, & Seong B.L. (2018). Non-specific Effect of Vaccines: Immediate Protection against Respiratory Syncytial Virus Infection by a Live Attenuated Influenza Vaccine. Frontiers in Microbiology, 9, 83.

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