Rabbit anti gfp alexa 488

Manufactured by Thermo Fisher Scientific

Rabbit anti-GFP Alexa-488 is a primary antibody that specifically binds to green fluorescent protein (GFP). It is conjugated with the Alexa Fluor 488 fluorescent dye, which emits green fluorescence when excited by the appropriate wavelength of light. This antibody can be used for detection and localization of GFP-tagged proteins in various biological applications.

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Lab products found in correlation

Anti phospho p44 42 map kinase antibody, by Cell Signaling Technology (1 mentions) Blocking reagent, by Roche (1 mentions) Goat anti mouse igg alexa 568, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg alexa 568, by Thermo Fisher Scientific (1 mentions) Lsm 700 confocal microscope, by Zeiss (1 mentions)

Spelling variants of this product

Alexa 488 (1863 citations) Rabbit anti-GFP (584 citations) Anti-GFP (404 citations) Alexa anti-rabbit 488 (3 citations) Alexa Fluor® 488 conjugated rabbit anti-GFP (3 citations) Rabbit anti-GFP 488 (2 citations) Rabbit anti-GFP Alexa Fluor 488 (2 citations)

2 protocols using rabbit anti gfp alexa 488

Phospho-p44/42 MAPK Localization in Stab2 Morphant Embryos

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Control uninjected and Stab2 morphant embryos were fixed in 4% paraformaldehyde (PFA) at the 20 somite stage, dehydrated in ethanol and stored at −20°C. Embryos were rehydrated and blocked in blocking reagent (Roche). Following blocking, embryos were incubated in rabbit polyclonal anti-phospho-p44/42 MAP kinase antibody (Cell Signaling Technologies) overnight, washed, incubated in rabbit anti-Alexa647 (Invitrogen) for four hours, washed, and incubated in rabbit anti-GFP Alexa-488 (Invitrogen) overnight. Embryos were analyzed and imaged using fluorescent microscopy.

Rost M.S, & Sumanas S. (2014). Hyaluronic Acid Receptor Stabilin-2 Regulates Erk Phosphorylation and Arterial - Venous Differentiation in Zebrafish. PLoS ONE, 9(2), e88614.

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Antibody Staining Protocol for Confocal Microscopy

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Detailed protocols for antibody staining have been published previously (Lorent et al., 2004 (link)). Primary antibodies included rabbit anti-GFP-Alexa 488 (1:400, except 1:200 following fluorescence in situ hybridization) (Invitrogen), rabbit anti-Mdr (1:100, Santa Cruz Biotechnology), and mouse monoclonal 2F11 (1:1000, gift of Julien Lewis). Primary antibody incubations were performed overnight at 4°C. Secondary antibodies included goat anti-rabbit IgG-Alexa 568 and goat anti-mouse IgG-Alexa 568 (Invitrogen), and were diluted 1:600 for incubation at room temperature for 2–3 h. For confocal microscopy, livers and attached gastrointestinal tracts were dissected from immunostained larvae and mounted in Vectashield with DAPI (Vector Industries), coverslipped, and imaged using a Zeiss LSM 700 confocal microscope.

Wilkins B.J., Gong W, & Pack M. (2014). A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver. Gene expression patterns : GEP, 14(2), 62-68.

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