Uniformly labeled 13c glucose

Manufactured by Cambridge Isotopes

Sourced in United States

Uniformly labeled 13C glucose is a stable isotope-labeled compound used as a tracer in metabolic research. It contains carbon-13 (13C) atoms uniformly distributed across the glucose molecule, allowing for the tracking of glucose metabolism in biological systems.

Automatically generated - may contain errors

Lab products found in correlation

Unlabeled glucose, by Merck Group (2 mentions) 1 13c glucose, by Cambridge Isotopes (2 mentions) Tryptic soy agar, by Thermo Fisher Scientific (1 mentions) Ciprofloxacin cipro, by Merck Group (1 mentions) Mops ez rich defined medium, by Teknova (1 mentions)

Close spelling variants of this product

13C-glucose (50 citations) 13C-labeled glucose (18 citations)

5 protocols using uniformly labeled 13c glucose

Metabolomic Analysis of Glut1 Knockout Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Metabolomic analyses were performed as described before using la iquid chromatography Q Exactive Mass Spectrometer (LC-QE-MS) (Thermo Scientific).^{22 (link)} In non-targeted metabolomics analyses, Glut1^fl/fl CreER and WT CreER cells were treated with vehicle or 0.4 μM 4-OHT for 96 h followed by an additional 48 h in complete IMDM media containing 1 mM sodium pyruvate. For 13C-glucose flux studies, cells were cultured as described above for 5 days culture, washed with PBS (Mediatech), and cultured in glucose-free RPMI without sodium pyruvate (Gibco) supplemented with 10% fetal bovine serum, glutamine (2 mM) and uniformly labeled 13C-glucose (10 mM; Cambridge Isotope Laboratories, Tewksbury, MA, USA) for 24 h. Metaboanalyst was used to range-scale data and provide PCA and KEGG pathway analysis of metabolites significantly changed (1.5-fold difference, P<0.05)(www.metaboanalyst.ca/).^{43 (link)}

Liu T., Kishton R.J., Macintyre A.N., Gerriets V.A., Xiang H., Liu X., Abel E.D., Rizzieri D., Locasale J.W, & Rathmell J.C. (2014). Glucose transporter 1-mediated glucose uptake is limiting for B-cell acute lymphoblastic leukemia anabolic metabolism and resistance to apoptosis. Cell Death & Disease, 5(10), e1470-.

+ Open protocol

+ Expand

Glucose labeling for metabolic analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Uniformly labeled ¹³C glucose and 1-¹³C glucose were from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). Unlabeled glucose and other reagents were from Sigma-Aldrich (St. Louis, MO). LC–MS/MS reagents were from Honeywell Burdick & Jackson® (Muskegon, MI), Fisher Scientific (Pittsburgh, PA) and Sigma-Aldrich (St. Louis, MO).

McCloskey D., Xu S., Sandberg T.E., Brunk E., Hefner Y., Szubin R., Feist A.M, & Palsson B.O. (2018). Evolution of gene knockout strains of E. coli reveal regulatory architectures governed by metabolism. Nature Communications, 9, 3796.

+ Open protocol

+ Expand

Metabolic Profiling with Ciprofloxacin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ciprofloxacin (cipro) and all metabolites used as metabolic
perturbations or metabolomic profiling standards were purchased from
Sigma-Aldrich (St. Louis, MO). BD Difco TSB and tryptic soy agar were
purchased from Fisher Scientific (Hampton, NH). Uniformly labeled
¹³C glucose was purchased from Cambridge Isotope
Laboratories, Inc. (Tewksbury, MA). LC-MS reagents were purchased from
Honeywell Burdick & Jackson® (Muskegon, MI) and
Sigma-Aldrich.

Yang J.H., Bhargava P., McCloskey D., Mao N., Palsson B.O, & Collins J.J. (2017). Antibiotic-Induced Changes to the Host Metabolic Environment Inhibit Drug Efficacy and Alter Immune Function. Cell host & microbe, 22(6), 757-765.e3.

+ Open protocol

+ Expand

E. coli Cultivation and Metabolite Supplementation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Escherichia coli strain K-12 MG1655 (ATCC 700926) was used for all experiments in this study. For metabolite supplementation experiments, cells were cultured in MOPS minimal medium with 0.2% glucose (Teknova; Hollister, CA). For experiments involving gene deletions, cells were cultured in MOPS EZ Rich defined medium (Teknova). For all experiments, cells were grown at 37°C either on a rotating shaker at 300 rpm in baffled flasks or 14 mL test tubes or on a rotating shaker at 900 rpm in Biolog 96-well phenotype microarrays (Bochner, 2009 (link)) (Biolog; Hayward, CA). All experiments were performed with n ≥ 3 biological replicates from independent overnight cultures. Uniformly labeled ¹³C glucose was purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). LC-MS reagents were purchased from Honeywell Burdick & Jackson® (Muskegon, MI) and Sigma-Aldrich (St. Louis, MO).

Yang J.H., Wright S.N., Meagan H.a.m.b.l.i.n., McCloskey D., Alcantar M.A., Schrübbers L., Lopatkin A.J., Satish S., Nili A., Palsson B.O., Walker G.C, & Collins J.J. (2019). A white-box machine learning approach for revealing antibiotic mechanisms of action. Cell, 177(6), 1649-1661.e9.

+ Open protocol

+ Expand

Isotopic Glucose Labeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Uniformly labeled ¹³C glucose and 1-¹³C glucose was purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). Unlabeled glucose and other media components were purchased from Sigma-Aldrich (St. Louis, MO). LC-MS reagents were purchased from Honeywell Burdick and Jackson® (Muskegon, MI), Fisher Scientific (Pittsburgh, PA) and Sigma-Aldrich (St. Louis, MO).

McCloskey D., Xu S., Sandberg T.E., Brunk E., Hefner Y., Szubin R., Feist A.M, & Palsson B.O. (2018). Growth Adaptation of gnd and sdhCB Escherichia coli Deletion Strains Diverges From a Similar Initial Perturbation of the Transcriptome. Frontiers in Microbiology, 9, 1793.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!