Ds qi2 cmos camera

Manufactured by Nikon

Sourced in United States, Japan

The DS-Qi2 CMOS camera is a high-performance digital camera designed for microscopy applications. It features a 16.2-megapixel CMOS sensor and can capture images at a resolution of 4912 x 3264 pixels. The camera supports live-view functionality and offers a wide dynamic range for accurate image capture.

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Lab products found in correlation

Eclipse ti, by Nikon (4 mentions) Ixon ultra 897 emccd camera, by Oxford Instruments (3 mentions) Eclipse ti2 e microscope, by Nikon (3 mentions) Spectra x, by Lumencor (3 mentions) Nis elements software, by Nikon (3 mentions) T1 inverted fluorescence microscope, by Nikon (3 mentions) A1r hd, by Nikon (2 mentions) Lab tek eight well chambers, by Thermo Fisher Scientific (2 mentions) Element ar software, by Nikon (2 mentions) L 15 medium, by Thermo Fisher Scientific (1 mentions) Plan 0.40 na air objective, by Nikon (1 mentions) Eclipse ti2 inverted microscope, by Nikon (1 mentions) Prism 5, by GraphPad (1 mentions) Lambda 10 b smart shutter, by Sutter Instruments (1 mentions) Nis elements acquisition software, by Nikon (1 mentions) Spectra x led light engine, by Lumencor (1 mentions) Ti e inverted microscope, by Nikon (1 mentions) Jobs module, by Nikon (1 mentions) Igor pro environment, by Wavemetrics (1 mentions) Neo scmos camera, by Oxford Instruments (1 mentions)

Spelling variants of this product

DS-Qi2 camera (138 citations) DS-Qi2 (128 citations) DS-Qi2 monochrome CMOS camera (5 citations) CMOS camera (4 citations) Qi2 CMOS camera (2 citations)

21 protocols using ds qi2 cmos camera

Wide-field Imaging of Photoconverted Myosin-II

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High-resolution wide-field fluorescence images were acquired on a Nikon Eclipse Ti equipped with a Nikon 100× Plan Apo 1.45 numerical aperture (NA) oil objective and a Nikon DS-Qi2 CMOS camera. Images were acquired every 10 s using 100- to 300-ms integration using a fluorescein isothiocyanate (FITC) filter cube. Photoconversion of NMIIA-mEOS2 was accomplished by closing the Eclipse Ti’s field diaphragm and using a 4’,6-diamidino-2-phenylindole filter cube to shine UV light on the cell’s leading edge for 10 s as previously described (Baker et al., 2010 (link)). This was immediately followed by opening the field diaphragm and imaging with FITC and Tex Red filter cubes every 1 min. Phase imaging was performed on a Nikon Eclipse Ti equipped with a Nikon 20× Plan 0.40 NA air objective and Nikon DS-Qi2 CMOS camera. Images were acquired every 1-min using 50-ms integration. A custom-built incubator was constructed to keep cells cultured in L-15 medium (BW12700Q; Fisher Scientific) at 37°C. An orange glass filter was placed on top of the condenser to prevent the inactivation of blebbistatin (Sakamoto et al., 2005 (link)).

Fenix A.M., Taneja N., Buttler C.A., Lewis J., Van Engelenburg S.B., Ohi R, & Burnette D.T. (2016). Expansion and concatenation of nonmuscle myosin IIA filaments drive cellular contractile system formation during interphase and mitosis. Molecular Biology of the Cell, 27(9), 1465-1478.

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Visualizing p75NTR Dynamics in Neurons

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Sympathetic neurons were electroporated with mCherry-p75NTR-GFP construct and plated on glass bottom dishes. The neurons were first plated in UltraCULTURE media with 50 ng/ml NGF which was changed to media with 20 ng/ml NGF next day. After 2 days in culture, NGF was removed and the neurons were incubated with a p75NTR antibody (Anti-p75NTR (extracellular)-ATTO-550, Alomone labs) for 30 min in phenol red free DMEM with HEPES, BSA (1 mg/ml) and KCl (12.5 mM) on ice. The antibody was then rinsed off and the neurons were treated with or without 200 ng/ml BDNF +/− 5 μM MS275 for 2 hrs.
High-resolution wide-field fluorescence images were acquired on a Nikon Eclipse Ti equipped with a Nikon 100× Plan Apo 1.45 numerical aperture (NA) oil objective and a Nikon DS-Qi2 CMOS camera. Multichannel time lapse images were acquired at 3 sec intervals for a total of 3 min and processed using Nikon Imaging software to generate the kymographs. The cell body is at the top of the kymograph, all sloping (moving) pathways were counted to be moving particles while the horizontal pathways were considered to be stationary. All the kymographs were blindly analyzed to quantify the overall direction of movement. Approximately 100 moving particles were analyzed from at least 8 different neurons in each condition for each experiment.

Pathak A., Stanley E.M., Hickman F.E., Wallace N., Brewer B., Li D., Gluska S., Perlson E., Fuhrmann S., Akassoglou K., Bronfman F., Casaccia P., Burnette D.T, & Carter B.D. (2018). Retrograde degenerative signaling mediated by the p75 neurotrophin receptor requires p150Glued deacetylation by axonal HDAC1. Developmental cell, 46(3), 376-387.e7.

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Characterization of Polydisperse Droplets

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The volume values were obtained in the same procedure previously described. 13 Droplets were generated by vortexing the aqueous phase and non-aqueous phase together in a 1.5 mL microcentrifuge tube. Samples were thermocycled then transferred to an imaging chamber and characterized using a microscope (20× objective on a Nikon Eclipse Ti2 inverted microscope with epifluorescence illumination and a 14-bit Nikon DS-Qi2 CMOS camera (Nikon Instruments, Melville, NY, USA)). The stability of polydisperse droplets prepared using this same technique was previous demonstrated 13 and the stability of the reagents used in this work has been previously noted. 21, 22

Huynh T ., Byrnes SA ., Chang TC ., Weigl BH , & Nichols KP . (2019). General methods for quantitative interpretation of results of digital variable-volume assays. The Analyst, 144(24).

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Quantitative Fluorescent Microscopy Protocol

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Fluorescent microscopy was performed at the Nikon Imaging Core (UCSD) using a Nikon Eclipse Ti2-E microscope with Plan Apo objectives. Samples were excited by the Lumencor SpectraX and acquired with a DS-Qi2 CMOS camera using NIS-Elements software, or with a laser scanning confocal (A1R HD, Nikon), acquired with an iXon Ultra 897 EMCCD camera (Andor). All AVPV slides were imaged at the same time and under the same conditions. All ARC and thalamic reticular nucleus slides were imaged at the same time and under the same conditions. The number of Kiss1 cells that colocalized with Six3 or atypical signal was determined manually, using FIJI Cell Counter tool. NIS-Elements: General Analysis software was used to objectively quantify the intensity of Kiss1, cFos, and Six3 signals. A signal threshold of 2.5 standard deviations above background was used for defining positive signal of each gene. cFos and Six3 signals were quantified only in Kiss1-positive cells.

Lavalle S.N., Chou T., Hernandez J., Naing N.C., He M.Y., Tonsfeldt K.J, & Mellon P.L. (2022). Deletion of the homeodomain gene Six3 from kisspeptin neurons causes subfertility in female mice. Molecular and cellular endocrinology, 546, 111577.

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Ratiometric FRET Imaging of AKAR4 Biosensor

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For sensitized emission measurements of AKAR4 biosensor in HeLa cells wide-field images were captured with a Nikon TiE inverted microscope with a 20 × 0.5NA objective and a DS-Qi2 CMOS camera (Nikon, Japan). Images were acquired at intervals of 2 min with the Nikon NIS-Elements acquisition software using JOBS module (Nikon, Japan). A Lumencor Spectra X LED Light Engine (Lumencor, Beaverton, OR, USA) was used as excitation light source to reduce phototoxicity. Ratio imaging used a 440/30 nm excitation filter, a t440/510/575rpc multi-band dichroic mirror, and two emission filters (ET480/40 M (cyan fluorescent protein: CFP) and AT545/30 M (FRET)). Lumencor provided excitation filters, and all dichroic mirrors and emission filters were obtained from Chroma Technology (Brattleboro, VT, USA). An automated emission filter wheel Lambda 10-B Smart Shutter (Sutter Instrument, Novato, CA, USA) was used. The F480/F545 emission ratio, indicative of biosensor activation, calculated for each pixel on the whole image, was performed with custom routines written in IGOR Pro environment (Wavemetrics, Lake Oswego, OR, USA). Normalized F480/F545 emission ratio values were then plotted in PrismV (GraphPad software, La Jolla, CA, USA) and displayed in two ways: cell trace for each individual cell (grey) overlaid with the population mean in red and the 25^th and 75^th percentile values in blue.

Demeautis C., Sipieter F., Roul J., Chapuis C., Padilla-Parra S., Riquet F.B, & Tramier M. (2017). Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM. Scientific Reports, 7, 41026.

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Quantifying Cytoskeletal Dynamics via Microscopy

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High-resolution widefield fluorescence images (for Z-line measurement) and live imaging of actin filaments (to measure MSF translocation rates) were acquired on a Nikon Eclipse Ti equipped with a Nikon 100× Plan Apo 1.45 numerical aperture (NA) oil objective and a Nikon DS-Qi2 CMOS camera. Live imaging for Figure 2 and focused laser-mediated cutting for Figure 1 were performed on a Nikon Spinning Disk confocal microscope equipped with a 60 × 1.4 NA objective and an Andor iXON Ultra EMCCD camera, provided by the Nikon Center of Excellence, Vanderbilt University. Cutting of DSFs was performed using a 100 mW UV laser (Coherent Technologies) at 75% power, using a dwell time of 500 µs for a total period of 1 s. TIRF imaging was performed using a Nikon TiE inverted light microscope equipped with a 100 × 1.49 NA TIRF objective and an Andor Neo sCMOS camera. Cells were maintained at 37°C with 5% CO₂ using a Tokai Hit stage incubator.

Taneja N., Neininger A.C, & Burnette D.T. (2020). Coupling to substrate adhesions drives the maturation of muscle stress fibers into myofibrils within cardiomyocytes. Molecular Biology of the Cell, 31(12), 1273-1288.

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Quantifying Protein Recruitment to Centrosomes

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U2Os cells were transfected with the indicated constructs as described above. Cells transfected with YFP‐FKBP‐tagged diffusion probes and Ce3‐FRB‐tagged CTSs were imaged every 5 s for 4 min on a Nikon T1 inverted fluorescence microscope with a 60× oil objective (Nikon), DS‐Qi2 CMOS camera, and 37°C, 5% CO2 heated stage. We added 100 nM rapamycin (LC Laboratories) during imaging. Time‐lapse images were processed by Huygens Deconvolution software. The intensity of YFP‐FKBP‐tagged proteins at centrosomes upon rapamycin treatment was measured by Nikon Elements AR software.

Cheng H., Kao Y., Chen T., Sharma L., Yang W., Chuang Y., Huang S., Lin H., Huang Y., Kao C., Yang L., Bearon R., Cheng H., Hsia K, & Lin Y. (2022). Actin filaments form a size‐dependent diffusion barrier around centrosomes. EMBO Reports, 24(1), e54935.

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Fluorescence Microscopy of Animal Tissue

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Fluorescence microscopy for the animal tissue was performed at the Nikon Imaging Core (UCSD) using a Nikon Eclipse Ti2-E microscope with Plan Apo objectives. Samples were excited by the Lumencor SpectraX and acquired with a DS-Qi2 CMOS camera using NIS-Elements software (Nikon) acquired with an iXon Ultra 897 EMCCD camera (Andor). All slides were imaged under the same conditions. The number of tdTomato⁺ cells that colocalized with SOX2 was determined manually, using FIJI Cell Counter tool. Fluorescence microscopy for the in vitro work was performed at the UCSD Neuroscience Microscopy Imaging Core with a Keyance BZ-X Series all-in-one fluorescence microscope using Keyance software.

Cassin J., Stamou M.I., Keefe K.W., Sung K.E., Bojo C.C., Tonsfeldt K.J., Rojas R.A., Ferreira Lopes V., Plummer L., Salnikov K.B., Keefe DL J.r., Ozata M., Genel M., Georgopoulos N.A., Hall J.E., Crowley WF J.r., Seminara S.B., Mellon P.L, & Balasubramanian R. (2023). Heterozygous mutations in SOX2 may cause idiopathic hypogonadotropic hypogonadism via dominant-negative mechanisms. JCI Insight, 8(3), e164324.

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Live-cell imaging of cytoskeletal dynamics

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Neon-IFT88 or Neon-Kif3B-stable NIH3T3 cells were transfected with the indicated constructs and then seeded onto poly(d-lysine)-coated borosilicate glass Lab-Tek eight-well chambers (Thermo Scientific). The cells were imaged every 200 ms for 30 s on a Nikon T1 inverted fluorescence microscope (Nikon) with a ×60 oil objective (Nikon), DS-Qi2 CMOS camera (Nikon), and 37 °C, 5% CO₂ heat stage (Live Cell Instrument). Time-lapse images were processed by Huygens deconvolution (Scientific Volume Imaging), and kymographs were produced with ImageJ and the plug-in KymographClear^{70 (link)}.

Hong S.R., Wang C.L., Huang Y.S., Chang Y.C., Chang Y.C., Pusapati G.V., Lin C.Y., Hsu N., Cheng H.C., Chiang Y.C., Huang W.E., Shaner N.C., Rohatgi R., Inoue T, & Lin Y.C. (2018). Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling. Nature Communications, 9, 1732.

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Live-cell Imaging with Nikon Microscope

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Live‐cell imaging was carried out with a Nikon T1 inverted fluorescence microscope with a 60× or 100× oil objective (Nikon), DS‐Qi2 CMOS camera (Nikon), and 37°C, 5% CO₂ heated stage (Live Cell Instrument). Images with multiple z‐stacks were processed with Huygens Deconvolution software (Scientific Volume Imaging). Image analysis and the maximum intensity projections of images were generated with Nikon Elements AR software.

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