Hm355s

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom

The HM355S is a high-quality centrifuge designed for laboratory applications. It features a robust construction and a versatile rotor system that can accommodate a variety of sample tubes and microplates. The centrifuge offers precise speed control and can reach a maximum speed of 6,000 RPM, making it suitable for a wide range of sample preparation and separation tasks.

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Lab products found in correlation

Asp300, by Leica (6 mentions) Axio observer z1, by Zeiss (3 mentions) Eclipse te2000 u microscope, by Nikon (2 mentions) Cellsens dimension desktop software, by Olympus (2 mentions) Neutral buffered formalin, by Thermo Fisher Scientific (2 mentions) Superfrost plus, by Thermo Fisher Scientific (2 mentions) Formic acid, by Merck Group (2 mentions) Nanozoomer, by Hamamatsu Photonics (2 mentions) Bond max system, by Leica (2 mentions) Scn400 slide scanner, by Leica (2 mentions) Anti hbcag primary antibody, by Diagnostic BioSystems (2 mentions) Superfrost plus microscope slide, by Thermo Fisher Scientific (2 mentions) Mx 20, by Hologic (2 mentions) Mol decalcifier, by Milestone (2 mentions) Kos histostation, by Milestone (2 mentions) Dm500, by Leica (2 mentions) Hematoxylin and eosin, by Merck Group (1 mentions) Shandon excelsior es, by Thermo Fisher Scientific (1 mentions) Eclipse ti, by Nikon (1 mentions) Osteosoft, by Merck Group (1 mentions)

Close spelling variants of this product

HM355S microtome (18 citations) Microtome Microm HM355S (3 citations) Micron HM355S (3 citations) Thermo/Microm HM355S (2 citations)

70 protocols using hm355s

Spatial and Single-Cell Profiling of Breast Cancer

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Sample #1. A single formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue block (TNM stage T2N1M0, ER + /HER2 + /PR −) was collected on 2021-07-26 and obtained from Discovery Life Sciences. Corresponding dissociated tumor cells, fresh frozen in liquid nitrogen, were also sampled from the same biopsy (patient matched). 5 μm sections were taken from the FFPE tissue using a microtome (Thermo Scientific HM355S; MX35 blades). For the Chromium Single Cell Gene Expression Flex (scFFPE-seq) workflow, 25 μm FFPE curls were collected into a tube prior to serial sectioning for Visium CytAssist and Xenium (two replicates of 5 μm sections for each spatial platform), then an additional 25 μm FFPE curl was collected into the same tube reserved for scFFPE-seq. These pooled 25 μm curls (50 μm total) were treated as a single replicate. Another replicate could not be performed due to the large amount of input material required by scFFPE-seq and needing to reserve the same block for multiple technologies.
Sample #2. A formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue block (AJCC pathologic stage pT2 pN1a pMX, ER − /HER2 + /PR −) was collected on 2009-07-24 and obtained from Discovery Life Sciences. 5 μm sections were taken from the FFPE tissue using a microtome (Thermo Scientific HM355S; MX35 blades).

Janesick A., Shelansky R., Gottscho A.D., Wagner F., Williams S.R., Rouault M., Beliakoff G., Morrison C.A., Oliveira M.F., Sicherman J.T., Kohlway A., Abousoud J., Drennon T.Y., Mohabbat S.H, & Taylor S.E. (2023). High resolution mapping of the tumor microenvironment using integrated single-cell, spatial and in situ analysis. Nature Communications, 14, 8353.

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Histological Processing of Mouse Lymph Nodes

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Mouse lymph nodes were fixed in 10% neutral-buffered formalin solution for min. 48 h, dehydrated under standard conditions (Leica ASP300S, Wetzlar, Germany) and embedded in paraffin. Serial 2 µm-thin sections prepared with a rotary microtome (HM355S, Thermo Fisher Scientific, Waltham, USA) were collected and subjected to histological and immunohistochemical analysis. Hematoxylin–Eosin (H–E) staining was performed on deparaffinized sections with Eosin and Mayer’s Haemalaun according to a standard protocol.

Schick M., Zhang L., Maurer S., Maurer H.C., Isaakaidis K., Schneider L., Patra U., Schunck K., Rohleder E., Hofstetter J., Baluapuri A., Scherger A.K., Slotta-Huspenina J., Hettler F., Weber J., Engleitner T., Maresch R., Slawska J., Lewis R., Istvanffy R., Habringer S., Steiger K., Baiker A., Oostendorp R.A., Miething C., Lenhof H.P., Bassermann F., Chapuy B., Wirth M., Wolf E., Rad R., Müller S, & Keller U. (2022). Genetic alterations of the SUMO isopeptidase SENP6 drive lymphomagenesis and genetic instability in diffuse large B-cell lymphoma. Nature Communications, 13, 281.

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Histological Analysis of Dissected Tumors

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Dissected tumor plugs were kept overnight in freshly prepared formalin solution and dehydrated in tissue processor (Shandon Excelsior ES, Thermo Scientific, Kalamazoo, MI, USA) using reagents provided by DiaPath (DiaPath, Martinengo, Italy). Dehydrated plugs were embedded in paraffin, cut into 4-μm-thick sections on microtome (HM 355S, Thermo Scientific, Kalamazoo, MI, USA), and stained with hematoxylin and eosin (Sigma-Aldrich, St. Louis, MO, USA) in Varistain Gemini automated slide stainer (Thermo Scientific, Kalamazoo, MI, USA), according to a standard protocol. Prepared sections were analyzed under a microscope (Eclipse Ti, Nikon, Tokyo, Japan). Pictures were taken using NIS-Elements BR software. Content of necrotic tissue was assessed using AxioVision 40 V 4.8.2 software (Carl Zeiss Microscopy, Jena, Germany).

Szade K., Zukowska M., Szade A., Collet G., Kloska D., Kieda C., Jozkowicz A, & Dulak J. (2015). Spheroid-plug model as a tool to study tumor development, angiogenesis, and heterogeneity in vivo. Tumour Biology, 37(2), 2481-2496.

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Femur Histology and Decalcification Protocol

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The right femurs were fixed in 4% buffered formalin for one day, and then decalcified with 9% formic acid for about three weeks. Attempts were made to standardize the sectioning at a mid-sagittal plane of each specimen by cutting the specimen in half (longitudinally in a sagittal plane) using a slicing blade. Samples were subjected to tissue processing and then embedded in paraffin. Thin sections (7 μm) were cut on a Rotary Microtome (HM 355S, Thermo Fisher Scientific, Inc., Germany) along the long axis of each femur in sagittal plane. Sections were mounted on the coated slides. Paraffin was removed by immersing the slides in Xylene 2 changes of 5 minutes at room temperature. Slides were then taken through graded ethanol and distilled water, then stained with Hematoxylin and Eosin (H&E), at last dehydrated and mounted.

Huang S., Xu L., Sun Y., Lin S., Gu W., Liu Y., Zhang J., Chen L, & Li G. (2016). Systemic Administration of Allogeneic Mesenchymal Stem Cells Does Not Halt Osteoporotic Bone Loss in Ovariectomized Rats. PLoS ONE, 11(10), e0163131.

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Formalin Fixation and Demineralization of Pulp Sphere-Seeded Root Canals

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The pulp sphere-seeded root canals were fixed in 3.7% neutral buffered formalin at 4 °C for 7 days, followed by demineralization in Osteosoft (Merck Millipore, Darmstadt, Germany) at room temperature for one week. After dehydration in ascending concentrations of alcohol and degreasing the samples twice in xylene, the dentin samples were embedded in paraffin. Sections of 5 μm thickness were cut by the use of a rotary microtome HM 355S (Thermo Fisher Scientific GmbH, Dreieich, Germany).

Neunzehn J., Pötschke S., Hannig C., Wiesmann H.P, & Weber M.T. (2017). Odontoblast-like differentiation and mineral formation of pulpsphere derived cells on human root canal dentin in vitro. Head & Face Medicine, 13, 23.

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Quantifying Macrophage Infiltration in Spleen and Myocardium

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For immunohistochemical studies, microtome sections of the spleen and myocardium were prepared using an HM 355S rotary microtome (Thermo Fisher Scientific, USA). From each block, 10 sections of spleen fragments and 20 sections of the myocardium were obtained. The material was applied to glasses coated with L-polylysine (two sections per glass). Two independent researchers investigated the macrophage infiltration of the spleen and myocardium via immunohistochemical studies conducted using an automatic immunostainer (Leica Bond-Max, Wetzlar, Germany). Macrophage immunophenotyping was performed using mouse monoclonal antibodies for the common macrophage marker CD68 (Cell Marque, dilution 1:500), and antibodies for the M2 macrophage marker synthesized in the Laboratory of Innate Immunity and Immunological Tolerance (University of Heidelberg) against stabilin-1 (dilution 1:1000) [14 (link)].
The studied markers were visualized using the HRP-DAB system (horseradish peroxidase-3, 3′-diaminobenzidine, peroxidase-3, 3′diaminobenzidine). Immunohistochemical staining was performed in accordance with the standard protocol [14 (link)]. Two independent researchers counted cells in both organs in 10 randomly chosen fields of view (40× objective) using a Zeiss Axio Imager M2 microscope, bright field (Figure 2).

Kercheva M., Ryabov V., Trusov A., Stepanov I, & Kzhyshkowska J. (2022). Characteristics of the Cardiosplenic Axis in Patients with Fatal Myocardial Infarction. Life, 12(5), 673.

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Analyzing Femoral Cartilage Mitochondrial Function

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Femora were isolated and fixed with 4% paraformaldehyde for 18 h. Fixed samples were decalcified using 0.5 M EDTA (pH 8.0), embedded in paraffin, and sectioned into 7-μm sections using a microtome (HM355 S; Thermo Fisher Scientific). The morphological organization of the PFE was evaluated on deparaffinized sections by safranin O staining (0.1% safranin O; Sigma–Aldrich). Activity of the mitochondrial complex IV (CYTOCOX) and complex II (SDH) was assessed on cryoembedded tissue. Isolated cartilage of the proximal femoral end was embedded in optimal cutting temperature compound medium (Tissue-Tek; Sakura), shock frozen in liquid nitrogen, and sectioned using the CM3050 cryostat (Leica Biosystems). About 7-μm cryosections were stained with a 1 mg/ml 3,3-diaminobenzidine (Sigma–Aldrich) solution for 30 min at 37 °C to visualize CYTOCOX activity (43 (link)). After washing with PBS, sections were treated with 2 mg/ml nitrotetrazolium blue chloride (Sigma–Aldrich) solution containing 0.2 M sodium succinate (Sigma–Aldrich) and 50 mM MgCl₂ (Merck KGaA) for 2 h at 37 °C to detect SDH activity. Stained sections were embedded in Kaiser's glycerol gelatine (Merck KGaA) and analyzed using a Nikon Eclipse TE2000-U microscope (Nikon).

Bubb K., Holzer T., Nolte J.L., Krüger M., Wilson R., Schlötzer-Schrehardt U., Brinckmann J., Altmüller J., Aszodi A., Fleischhauer L., Clausen-Schaumann H., Probst K, & Brachvogel B. (2021). Mitochondrial respiratory chain function promotes extracellular matrix integrity in cartilage. The Journal of Biological Chemistry, 297(4), 101224.

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SARS-CoV-2 Detection in Human Lungs

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Human lung tissues fixed in 4% paraformaldehyde were embedded in paraffin and sectioned at 4-μm thickness with a Thermo Fisher Scientific HM 355S rotary microtome. Deparaffinization and antigen retrieval were performed prior to immunofluorescence staining. Briefly, tissue sections were incubated with 0.1% Sudan Black B solution and blocked with 1× PBS containing 5% bovine serum albumin and 0.1% Triton X-100. Polyclonal rabbit primary antibodies were raised against the SARS-CoV-2 N glycoprotein (1:800), which cross-reacted with BtCoV-WIV1 and PCoV-GX. Anti-CD68 antibodies were used for macrophages, anti-podoplanin antibodies were used for type I pneumocytes, and anti-TTF1 antibodies were used for type II pneumocytes. FITC-conjugated Affinipure goat anti-rabbit IgG (H+L) (1:100) and Cy3-conjugated Affinipure goat anti-rabbit IgG (H+L) (1:100) were used. The stained slices were imaged using a Panoramic MIDI system (3DHISTECH).

Yang Y., Shen X.R., Zhang Y.L., Jiang R.D., Wang X., Guan Z.Q., Li Q., Yao Y.L., Gong Q.C., Geng R., Wang Q., Zhu Y., Luo J.Y., Shi Z.L., Zhang H.L., Peng K, & Zhou P. (2023). Strategy To Assess Zoonotic Potential Reveals Low Risk Posed by SARS-Related Coronaviruses from Bat and Pangolin. mBio, 14(2), e03285-22.

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Quantifying Copper in Rat Liver Tissue

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For laser ablation ICP-MS analysis, tissue sections of rat liver samples embedded in paraffin were prepared with a thickness of 5 μm using a microtome HM 355S (Thermo Scientific, Bremen, Germany). To quantify the copper concentration in the tissue samples, matrix-matched standards based on 10% gelatin in aqueous solutions of copper (II) sulfate pentahydrate were prepared as described before.⁶² (link) The concentration range for copper was between 10 and 1000 μg/g. To validate the standard concentrations, bulk analysis after digestion with nitric acid was used as described before.⁶² (link) A laser ablation system (LSX-213 G2+; Teledyne CETAC Technologies, Omaha, NE) was used. ICP-MS detection was performed with a quadrupole-based iCAP TQ (Thermo Fisher Scientific). The laser ablation and ICP-MS were connected with Tygon tubing Saint-Gobain (Courbevoie, France). The following ICP-MS parameters were applied for all measurements: forward power, 1550 W; cool gas flow, 14 L/min; and auxiliary gas flow, 0.8 L/min. In-house–developed software was used to convert the laser ablation ICP-MS data into 2-dimensional images. The copper concentration was calculated using a linear calibration function derived from the average signal intensities for each standard using Microsoft Excel 2016 (Microsoft Corp, Redmond, WA).

Einer C., Leitzinger C., Lichtmannegger J., Eberhagen C., Rieder T., Borchard S., Wimmer R., Denk G., Popper B., Neff F., Polishchuk E.V., Polishchuk R.S., Hauck S.M., von Toerne C., Müller J.C., Karst U., Baral B.S., DiSpirito A.A., Kremer A.E., Semrau J., Weiss K.H., Hohenester S, & Zischka H. (2018). A High-Calorie Diet Aggravates Mitochondrial Dysfunction and Triggers Severe Liver Damage in Wilson Disease Rats. Cellular and Molecular Gastroenterology and Hepatology, 7(3), 571-596.

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Golgi Staining of Cortical Neurons

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Golgi staining was carried out using the superGolgi Kit (Bioenno Tech, LLC, Santa Ana, CA, USA). Briefly, the mice were anesthetized and the intact brains were removed. The brains were immersed in the impregnation solution. The solution was replaced the next day and the brains were immersed 10 or 12 additional days in the dark, and then transferred to the post-impregnation buffer for 48 h in the dark. Coronal sections (80 μm thick) were cut using a microtome (HM 355S, Thermo Fisher Scientific, Houston, TX, USA). Sections were mounted on adhesive microscope slides using staining solution and post-staining buffer, cleaned, and cover slipped using Permount (FUJIFILM Wako Pure Chemical Corporation). Dendrites of cortical neurons were examined using the Eclipse Ni-U microscope (Nikon Corporation, Tokyo, Japan), and the images were captured with a CCD camera (DS-Fi2, Nikon Corporation, Tokyo, Japan).
For quantification of spines, all images were converted to 8-bit, inverted, and background subtracted using Fiji ImageJ (National Institutes of Health, Bethesda, MD, USA) software, and dendritic spine density was measured by two independent researchers. In each animal, three intact dendritic spines of cortical neurons were selected for more detailed observation.

Liu X., Zhou Q., Zhang J.H., Wang K.Y., Saito T., Saido T.C., Wang X., Gao X, & Azuma K. (2021). Microglia-Based Sex-Biased Neuropathology in Early-Stage Alzheimer’s Disease Model Mice and the Potential Pharmacologic Efficacy of Dioscin. Cells, 10(11), 3261.

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