Ft ir 6100

The microstructure of the samples was characterized using scanning electron microscopy (SEM, S-3500N, Hitachi, Tokyo, Japan), Fourier-transform infrared spectroscopy (FT-IR, FT/IR-6100, JASCO, Tokyo, Japan), X-ray photoelectron spectroscopy (XPS, KRATOS, Shimadzu, Kyoto, Japan) and field emission transmission electron microscope (FE-TEM, JEM-F200, JEOL Ltd., Tokyo, Japan). For SEM, the surfaces of the samples were coated with a Au/Pd thin film by ion sputtering (E-101, Hitachi). For FT-IR, the milled samples were mixed with KBr powder at the mass ratio of 1:60. The powder mixture was pressed to form a thin film for measurements. For FE-TEM, samples were supported on a copper mesh coated with triacetylcellulose and carbon and acceleration voltage was fixed at 200kV. The surface charge of the samples in 10 mM NaCl solution was measured using a zeta-potential analyzer (ELS-Z, Otsuka Electronics Co., Osaka, Japan). The amount of amino groups on the sample surface was determined using the ninhydrin reaction. Namely, the microspheres were reacted with the ninhydrin reagent, and the change in absorbance at 570 nm was examined using ultraviolet – visible (UV – vis) spectrophotometry (V-630, JASCO).

For detecting the structural characteristic of main functional groups in the nano-silver solution FTIR spectrometer (JASCO, FT/IR- 6100) was applied in the range of 400–4000 cm-1 at a resolution of 4 cm⁻¹, by employing KBr pellet technique.

The infrared spectra of almotriptan, buccal film (FA4), physical mixture and placebo film were recorded on FTIR spectrophotometer (FT/IR-6100, Jasco, Tokyo, Japan). An intimate mixture of samples was prepared by grinding with anhydrous potassium bromide powder at 1:5 (sample: KBr) ratio and the pellets were prepared using a hydraulic press (5000–10,000 psi). The spectral scan of the discs were drawn in transmittance mode between the ranges of 400–4000 cm⁻¹.