LX2 cells (human hepatic stellate cells; HSCs) were provided by Dr. Haeng Ran Seo (Institute Pasteur Korea). HUVEC cells (human umbilical vein endothelial cells) were obtained from Lonza (Basel, Switzerland). WI38 cells (human fibroblast cell line) were obtained from the Korean Cell Line Bank. The cells were maintained at 37 °C in a humidified atmosphere (5% CO2/95% air). LX2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with heat-inactivated 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1× penicillin (Welgene, Gyeongsan, Korea) (Complete media). HUVEC cells were maintained in Medium 200 (Gibco, USA) supplemented with 1× penicillin (Welgene, Gyeongsan, Korea), 1× LSGS (Gibco, USA) and heat-inactivated 10% FBS. For WI38 cells, Roswell Park Memorial Institute medium (RPMI 1640; Gibco) supplemented with 1× penicillin (Welgene, Gyeongsan, Korea), and 10% heat-inactivated FBS was used. Primary HCC cells were maintained in DMEM/F12 (Gibco, USA) supplemented with 1× penicillin (Welgene, Gyeongsan, Korea), 1× GlutaMmax (Gibco, USA), 1× Primocin (Invitrogen, Carlsbad, CA, USA), and 10% heat-inactivated FBS.
Penicillin
Manufactured by Welgene
Sourced in United States, Cameroon, Australia, Germany, United Kingdom
Penicillin is a type of laboratory equipment used for the cultivation and isolation of the Penicillium fungus, which is the source of the antibiotic compound penicillin. This equipment provides a controlled environment for the growth and production of penicillin.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Welgene (232 mentions)
Fetal bovine serum (fbs), by Welgene (72 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (48 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (47 mentions)
Rpmi 1640 medium, by Welgene (27 mentions)
Rpmi 1640, by Welgene (24 mentions)
L glutamine, by Welgene (19 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (16 mentions)
Dulbecco s modified eagle s medium, by Welgene (15 mentions)
Amphotericin b, by Welgene (13 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (12 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (9 mentions)
Rpmi 1640, by Thermo Fisher Scientific (8 mentions)
Lipopolysaccharides (lps), by Merck Group (7 mentions)
Trypsin, by Welgene (7 mentions)
Heat inactivated fetal bovine serum, by Welgene (7 mentions)
Penicillin, by Thermo Fisher Scientific (7 mentions)
Streptomycin, by Thermo Fisher Scientific (7 mentions)
Phosphate buffered saline (pbs), by Welgene (7 mentions)
Dmem f12, by Thermo Fisher Scientific (7 mentions)
266 protocols using penicillin
1
Cell Culture Protocols for Hepatic, Endothelial, and Fibroblast Cells
2
Cultivation of Prostate Cancer Cell Lines
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Human PCa DU145 cells from the American Type Culture Collection (ATCC, USA) were maintained in MEM media (Welgene, Korea) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin (Welgene) at 37°C with 5% CO2. LNCaP (ATCC) and 22RV1 (ATCC) cells were maintained in RPMI-1640 media (Welgene) supplemented with 10% FBS (Thermo Fisher Scientific), 100 U/ml penicillin, and 100 μg/ml streptomycin (Welgene) at 37°C with 5% CO2. PC3 (ATCC) cells were maintained in F12-K media (Welgene) supplemented with 10% FBS (Thermo Fisher Scientific), 100 U/ml penicillin, and 100 μg/ml streptomycin (Welgene) at 37°C with 5% CO2. LNCaP and 22RV1 cells are AR-positive, whereas DU145 and PC3 cells are AR-negative.
3
Culturing Human Hepatic Stellate and Monocyte Cells
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Example 6
Human Hepatic Stellate cell line, LX-2, was purchased from Sigma-Aldrich (St Louis, Mo., USA). The LX-2 cells were maintained in DMEM containing 2% fetal bovine serum (Life Technologies, Gaithersburg, Md., USA), 100 IU/ml penicillin, and 100 μg/ml streptomycin (Welgene, Daegu, South Korea).
Human Monocyte cell line, THP-1, was purchased from American Type Culture Collection (Manassas, Va., USA). The THP-1 cells were maintained in RPMI1640 containing 10% fetal bovine serum (Life Technologies, Gaithersburg, Md., USA), 100 IU/ml penicillin, 100 μg/ml streptomycin (Welgene, Daegu, South Korea), and 0.55 uM 2-Mecaptpethanol. Cell were cultured in both conditions, one condition is a humidified atmosphere at 37° C. containing 5% CO2, and another is hypoxia culture condition was maintained at 1% oxygen, 5% and 94%.
4
Culturing Human Cancer Cell Lines
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The MDA-MB-231 human BC cell line and Hep3B human HCC cell line were purchased from American Type Culture Collection (ATCC; Rockville, MD, USA). MDA-MB-231 cells were cultured in RPMI-1640 (WelGENE, Daegu, Korea) supplemented with 100 mL/L of fetal bovine serum (FBS; WelGENE), 100,000 U/L of penicillin (WelGENE), and 100 mg/L of streptomycin (WelGENE). Hep3B cells were grown in Dulbecco’s modified Eagle medium (DMEM, WelGENE) supplemented with 100 mL/L of FBS, 100,000 U/L of penicillin, and 100 mg/L of streptomycin as described above. Both cell lines were grown in a humidified atmosphere under 5% CO2 at 37 °C.
5
Culturing and Transfecting Human Colorectal Cancer Cell Lines
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The human colorectal adenocarcinoma LoVo, SW480 and DLD-1 cell lines were gifted by Eok-Soo Oh (Ewha Womans University). LoVo cells were maintained in Roswell Park Memorial Institute 1640 medium (RPMI-1640; #31800022; Gibco, Grand Island, NY, USA), SW480 cells were maintained in Dulbecco’s modified Eagle’s medium: Nutrient Mixture F-12 (DMEM/F-12; #12500062; Gibco) and DLD-1 cells were maintained in DMEM (#12100046; Gibco) supplemented with 10 % heat-inactivated fetal bovine serum (FBS; #US-FBS-500; GW Vitek, Seoul, South Korea), 100 units/ml penicillin and 100 µg/ml streptomycin (#LS202-02; WelGENE, Daegu, South Korea). In addition, HEK293T cells were maintained in DMEM supplemented with 10 % FBS (#US-FBS-500; GW Vitek), 100 units/ml penicillin and 100 ug/ml streptomycin (#LS202-02; WelGENE). The cells were incubated at 37 °C in a humidified incubator with 5 % CO2. For transient transfection, 1–3 µg of plasmids was transfected into HEK293T cells using the calcium phosphate precipitation method. SW480 cells were transfected using Lipofectamine 3000 Reagent (#L3000015; Invitrogen) according to the manufacturer’s instructions.
6
HaCaT Cell Culture and Stimulation
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The HaCaT cell line was obtained from the laboratory of Wonkwang university (Prof. Min Cheol Park). The HaCaT cells were incubated in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 units/ml of penicillin, and streptomycin (Welgene, Seoul, Korea). The HaCaT cells were cultured at 37°C in an incubator with a humidified atmosphere of 5% CO2 and 95% air. DMEM and FBS were purchased from GIBCO BRL (Grand Island, NY, USA). penicillin and streptomycin were purchased from Welgene (Seoul, Korea). Recombinant human TNF-α, IFN-γ, and IgE mouse ELISA kit were obtained from BioLegend (San Diego, CA, USA). Primary antibodies for Nrf2, Lamin B, HO-1, β-actin, and secondary antibodies used in the western blot analysis were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), dexamethasone (#D2915), and DNCB were purchased from Sigma-Aldrich. (St. Louis, Mo., USA).
7
Macrophage Culture: LX-2 and THP-1 Cell Lines
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Example 6
Macrophage Culture
Human Hepatic Stellate cell line, LX-2, was purchased from Sigma-Aldrich (St Louis, Mo., USA). The LX-2 cells were maintained in DMEM containing 2% fetal bovine serum (Life Technologies, Gaithersburg, Md., USA), 100 IU/ml penicillin, and 100 μg/ml streptomycin (Welgene, Daegu, South Korea).
Human Monocyte cell line, THP-1, was purchased from American Type Culture Collection (Manassas, Va., USA). The THP-1 cells were maintained in RPMI1640 containing 10% fetal bovine serum (Life Technologies, Gaithersburg, Md., USA), 100 IU/ml penicillin, 100 μg/ml streptomycin (Welgene, Daegu, South Korea), and 0.55 uM 2-Mecaptpethanol. Cell were cultured in both conditions, one condition is a humidified atmosphere at 37° C. containing 5% CO2, and another is hypoxia culture condition was maintained at 1% oxygen, 5% and 94%.
8
Culturing Human Hepatic Stellate and Monocyte Cells
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Example 6
Human Hepatic Stellate cell line, LX-2, was purchased from Sigma-Aldrich (St Louis, Mo., USA). The LX-2 cells were maintained in DMEM containing 2% fetal bovine serum (Life Technologies, Gaithersburg, Md., USA), 100 IU/ml penicillin, and 100 μg/ml streptomycin (Welgene, Daegu, South Korea).
Human Monocyte cell line, THP-1, was purchased from American Type Culture Collection (Manassas, Va., USA). The THP-1 cells were maintained in RPMI1640 containing 10% fetal bovine serum (Life Technologies, Gaithersburg, Md., USA), 100 IU/ml penicillin, 100 μg/ml streptomycin (Welgene, Daegu, South Korea), and 0.55 uM 2-Mecaptpethanol. Cell were cultured in both conditions, one condition is a humidified atmosphere at 37° C. containing 5% CO2, and another is hypoxia culture condition was maintained at 1% oxygen, 5% and 94%.
9
Cell Culture for Myogenic Differentiation
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C2C12 and HEK293T cell lines were obtained from American Type Culture Collection. C2C12 myoblasts were cultured in the growth medium, high glucose DMEM (Gibco, USA) containing 15% fetal bovine serum (Gibco, USA), 100 units/ml penicillin-100 μl/ml streptomycin (Welgene, South Korea). To induce myogenic differentiation, 80%-90% confluent C2C12 cells were exchanged into differentiation medium, high glucose DMEM containing 2% horse serum (Life Technologies, USA), 100 units/ml penicillin -100 μl/ml streptomycin (Welgene, South Korea). HEK293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum, 100 units/ml penicillin-100 μl/ml streptomycin. All cells were maintained at 37℃ under 5% CO2 in an incubator. Recombinant mouse interferongamma protein (R&D, USA), MG-132 (Sigma, USA), and NMS-873 (Sigma, USA) were prepared according to the manufacturer's instructions.
10
C2C12 Myoblast Differentiation Protocol
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C2C12 myoblasts (ATCC) were seeded onto non-coated 6-well plates and maintained in Dulbecco’s modified Eagle’s medium (DMEM, Welgene, Korea) containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 mg/mL streptomycin (Welgene, Korea) in a humidified atmosphere of 95% air and 5% CO2 at 37°C. For proliferation test, 5 mM L-carnitine or 300 uM oleic acid or a mixture (5 mM L-carnitine and 300 μM oleic acid) were added to the growth medium (10% FBS), based on previous studies15 (link),17 (link). When myoblasts were confluent (95%), the growth medium was changed to a differentiation medium (DM) supplemented with 2% horse serum (HS), 100 U/mL penicillin, and 100 mg/mL streptomycin (Welgene, Korea). DM was changed every 24 h. For the differentiation test and fusion index assay, 5 mM L-carnitine or 300 μM oleic acid or a mixture were added to DM supplemented with 2% HS and incubated for 96 h. The detailed incubation method for each experiment is described in each figure legend. Cell culture was performed a minimum of six independent times. As the fusion index is a myological indicator showing the degree of myogenic differentiation from myoblasts to myotubes, the index is calculated as the number of nuclei in myotubes divided by the total number of nuclei counted, which is expressed as a percentage. A schematic diagram of the cell culture protocol is presented in Figure 1 .
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