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2 protocols using pacap

1

Immunostaining of Synaptic Proteins

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Anti-ShhN (#2207; epitope surrounding Glu53 of human Shh) and cFos (#2250S) were from Cell Signaling Technology. Anti-ShhN (5E1) was from the Developmental Studies Hybridoma Bank. Anti-ShhC (#S9947; epitope-amino acids 199-437 of mouse Shh), anti-synaptophysin (#S5768), anti-map2 (#M9942) and anti-actin (#A5441 and #A2066) were from Sigma-Aldrich. smi312 antibody (#SMI-312R) was from Convance/BioLegend. Anti-bassoon (#141002), anti-homer1 (#160004), anti-Aldh1L1 (#278003), and anti-Gfap (#173002) were from Synaptic Systems. Anti-psd95 (#7E3-1B8) was from Thermo Fisher Scientific. Bicuculline (#0130) and PACAP (#1183) were from Tocris Bioscience. Purmorphamine (#ALX-420-045) was from Enzo Biochem Inc. Cytosine β-D-arabinofuranoside (#C1768) and Tetrodotoxin (#T5551) were from Sigma-Aldrich.
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2

Molecular Mediators in Vascular Regulation

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CGRP, amylin, PACAP, VIP, PGF, olcegepant, astemizole, ER819762 and Ro1138452 were from Tocris Bioscience (Bristol, UK); adrenomedullin, PACAP6-38, PGE2, PGI2 and histamine were from Sigma Aldrich (Milan, Italy); the mouse monoclonal anti-CGRP antibody (clone [4901]) and the inactive immunoglobulin (mouse monoclonal IgG2a) were from Abcam (Cambridge, UK)
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