A total of 10 mg of nanoICG was dispersed in 10 mL of lipiodol, and the crude lipiodol-nanoICG formulation was transferred to the clean SHIFT reactor [20 ]. After 1 h, the samples were collected and sterilized by irradiation (Fig. 1 B). The viscosities of lipiodol, the crude lipiodol–nanoICG (RL&nanoICG) formulation mixed using manual three-way blending, and SHIFT&nanoICG were detected with a viscosimeter at 37 °C. The radio-imaging properties of lipiodol and SHIFT&nanoICG were investigated using the Siemens Inveon micro-CT scanner (Munich, Germany).
Inveon micro ct scanner
Manufactured by Siemens
Sourced in United States, Germany
The Inveon micro-CT scanner is a high-resolution imaging system designed for preclinical research applications. It provides non-invasive, three-dimensional visualization of small animal anatomy and physiology. The Inveon micro-CT scanner captures detailed images of bone, soft tissue, and other structures, enabling researchers to study a wide range of biological processes.
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18 protocols using inveon micro ct scanner
1
Nanoparticle-Loaded Lipiodol for Imaging
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Micro-CT Analysis of Ossification
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A Siemens Inveon Micro CT scanner (Siemens Medical Solutions, Knoxville, TN, USA) was used to analyze the specimens using the following parameters: X-ray beam voltage, 80 kV; current, 500 μA; and effective resolution 13.6 μm. Referring to the semi-quantitative grading criteria of Rutges et al (14 (link)), the following criteria were adopted for this analysis: the absence of calcification was indicated as -; the presence of a single area of calcification as ±; the presence of 2 clear areas of calcification as +; and the clear presence of multiple areas of calcification as ++. We designated - or ± for a negative CT scan as the control group; ++ with positive CT scan as experiment group. At least 2 of the authors collaborated to assess the ossification from the CT radiograph and the ossification grade according to the micro CT analysis.
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Micro-CT Imaging of Tumor Metastasis
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Micro-computed tomography (CT) imaging was performed 2 weeks after the tumor cells were injected into the mice. Images were acquired using an Inveon Micro-CT scanner (Siemens Preclinical Solution, Knoxville, TN, USA). The animals were anesthetized with 2% isoflurane during the scan. The mice were placed in a prone position on the bed of the scanner, and two bed positions were acquired. The imaging program was a 5-min CT scan. Whole body images were obtained using the micro-CT scanner. The composite micro-CT images were generated using an Inveon Research Workplace (Siemens Preclinical Solution). For image reconstruction, the standard Ordered Subsets Expectation Maximization technique was used.
All of the mice were sacrificed 5 weeks after injection. The tumors and lungs were excised and embedded in paraffin for hematoxylin and eosin (H&E) staining.
All of the mice were sacrificed 5 weeks after injection. The tumors and lungs were excised and embedded in paraffin for hematoxylin and eosin (H&E) staining.
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Longitudinal Tumor Monitoring via MicroCT
5
Micro-CT Analysis of Lumbar Fusion
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Posteroanterior radiographs were taken of all the lumbar spines at 35 kV for 90 s. Two independent, blinded observers graded the spines on radiographs as showing a solid bilateral fusion mass (A) a unilateral fusion mass (B) a small bilateral fusion mass (C) or graft resorption (D) based on previously published radiographic scales. 27 Micro-Computed Tomography Analysis Micro-computed tomography (CT) analysis was used to quantitatively calculate new bone formation in all lumbar spine specimens. Areas of interest were demarcated from the top of the L4 transverse process cephalad to the bottom of the L5 transverse process caudally, including any bone lateral to a vertical line connecting the pars of the involved vertebrae (Fig. 1A andB). The spines were analyzed on both the right and left sides. In the three-dimensional analysis, tissue volume (TV), bone volume (BV), and bone volume fraction (BV/TV, %) were calculated. A Siemens Inveon Micro-CT scanner (Siemens, Germany) was used with a pixel size of 20 mm.
6
Muscle Strength and Bone Density Evaluation
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Before euthanasia, the muscle strength of limbs was assessed by a wire screen holding test described previously (Carlson et al., 2010 (link)). The muscle strength was evaluated by the physical impulse (Fdt) (N·s) = body mass (g) × 0.00980665 N/g × holding time(s). After euthanasia with an intraperitoneal injection of excess pentobarbital sodium, the femurs and tibias were harvest. And the Bone mineral density was measured using a Siemens Inveon Micro-CT scanner (Siemens Medical Solutions United States Inc, Malvern, PA, United States).
7
Micro-CT Imaging of Acute Lung Injury
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In the past decade, micro-computed tomography (CT) has become a powerful technique in laboratory investigation as technical advances in computer speed and memory have enabled micro-CT systems to generate high-spatial-resolution images of small specimens (17 (link)). To evaluate LPS responses to ALI, micro-CT images were obtained of the entire thorax using a Siemens Inveon micro-CT scanner (Siemens, Germany) after 48 h of LPS administration. The mice were anesthetized and placed on a plate in the supine position. CT images were collected on a volumetric CT scanner at 70 kVp and 500 μA. Images were acquired at 1,300 ms per frame and 360 views, and were reconstructed using the Feldkamp Algorithm. The reconstructed image was 2,048 × 2,048 pixels and effective pixel size was 39.99 μm. The final reconstructed data were converted to the Digital Imaging and Communications in Medicine format (Lucion; MeviSYS, Seoul, Korea).
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Micro-CT Imaging of Limb Bone Destruction
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Formalin-ethanol fixed hind limbs were micro-CT scanned prior to histology processing using a Siemens Inveon micro-CT scanner (Victoria, Australia). Data was acquired with the Inveon Acquisition Workplace at 16.84µm pixel size, 360 projections, 4100ms integration time, 80keV photon energy and 140µA current. 3D models were visualized and snap shots of the limbs acquired with the Inveon Research Workplace software. Data was quantified by binary score where 0=no bone destruction and 1=destruction was given to each individual limb.
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Radiographic and Microstructural Evaluation of Bone Healing
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X-ray examinations were performed under anesthesia at 4-, 8-, and 10-weeks post-surgery. The fracture images were blindly reviewed and scored by two individuals according to the radiographic scoring system adapted from other studies67 (link) (Table 2 ). To quantitatively evaluate bone formation at 10-weeks, microCT scanning of the healing femur was performed using Siemens Inveon microCT scanner (Siemens, Malvern, PA) at the Center for Magnetic Resonance Research at the University of Minnesota. To avoid the interference of the metal material, the intramedullary Kirschner wires were removed carefully before scanning. The harvested rat femurs were placed in polypropylene tubes filled with 70% ethanol, aligned orthogonally to the X-ray beam, and scanned at 80 kv and 500 uA with 720 steps for 360 degree rotation in a resolution of 35.84 μm and the pixel matrix 2368 × 3264. The images were acquired by Inveon Acquisition Workplace 1.5 and reconstructed with Inveon Research Workplace 4.2 associated with the scanner. In order to quantify the ratio of bone volume to total tissue volume (BV/TV) and trabecular bone thickness in the healing area, a standardized region of interest (ROI) in the reconstructed images was determined 2 mm above and below the fracture site with manually corrected contours to cross the gap in non-union fractures.
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Microstructural Mineralization Analysis of Abcc6 Rats
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