Anti lamin a c sc 6215

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-Lamin A/C (sc-6215) is a primary antibody product offered by Santa Cruz Biotechnology. It is a mouse monoclonal antibody that recognizes Lamin A and Lamin C proteins.

Automatically generated - may contain errors

Lab products found in correlation

Rapamycin r 5000, by LC Laboratories (3 mentions) Anti β actin a1978, by Merck Group (2 mentions) Alexa fluor 594 donkey anti mouse igg, by Thermo Fisher Scientific (2 mentions) Anti lc3, by Cell Signaling Technology (2 mentions) Anti tubulin lf pa0146a, by AbFrontier (2 mentions) Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Bafilomycin a1, by Cayman Chemical (2 mentions) Anti flag f3165, by Merck Group (2 mentions) Cycloheximide c4859, by Merck Group (1 mentions) Aicar a9978, by Merck Group (1 mentions) Phenformin p7045, by Merck Group (1 mentions) Anti ha antibody mms 101r, by Fortrea (1 mentions) Mg132 m 1157, by AG Scientific (1 mentions) Chloroquine c6628, by Merck Group (1 mentions) Bafilomycin a1 b1793, by Merck Group (1 mentions) Ha 2367, by Cell Signaling Technology (1 mentions) Anti heme oxygenase 1 adi spa 895, by Enzo Life Sciences (1 mentions) Anti lef1, by Santa Cruz Biotechnology (1 mentions) Anti tg2, by Cell Signaling Technology (1 mentions) Mab645, by R&D Systems (1 mentions)

9 protocols using anti lamin a c sc 6215

Antibody Characterization for Studying Nuclear Envelope Proteins

Cited 2 times

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Anti-human SUN1 2383 and anti-human SUN2 2853 antibodies have been described previously [44] (link). Anti-SUN1 Atlas antibody (HPA008346) was obtained from Sigma prestige antibodies. Anti-nesprin-2 (N2N3) antibody was kind gift from Q. Zhang (King's College London) and has been described previously [16] (link). Anti-nesprin-2G has been reported previously [53] (link). Anti-nesprin-2 monoclonal antibody (IQ562) was purchased from Immuquest. Monoclonal anti-emerin antibody was a kind gift from G. Morris (Center for Inherited Neuromuscular Disease, Oswestry, UK). Anti-lamin A/C (sc-6215) and GFP antibodies were purchased from Santa Cruz Biotechnologies. Anti- GAPDH (MAM374) was obtained from Millipore. Anti-α-tubulin (T9026), anti-β-actin (A5441), anti-γ-tubulin (T6557), anti-myc and anti-desmin antibodies were purchased from Sigma. Anti-caveolin 3 monoclonal antibody (610420) was purchased from Transduction Laboratories and anti-desmin polyclonal antibody (MONX10657) was purchased from Monosan. Anti-pericentrin polyclonal antibody (Ab4448) was obtained from Abcam.

Meinke P., Mattioli E., Haque F., Antoku S., Columbaro M., Straatman K.R., Worman H.J., Gundersen G.G., Lattanzi G., Wehnert M, & Shackleton S. (2014). Muscular Dystrophy-Associated SUN1 and SUN2 Variants Disrupt Nuclear-Cytoskeletal Connections and Myonuclear Organization. PLoS Genetics, 10(9), e1004605.

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Analysis of AMPK and autophagy regulation

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The following commercially available antibodies were used: anti-AMPKα1 (ab110036), anti-AMPKα2 (ab3760), anti-ATG14 (ab173943), anti-FOXO3a (ab12162), anti-histone H3 (ab1791), anti-H3R17me2 (ab8284), anti-H3K4me3 (ab8580), anti-H3K9me3 (ab8898), anti-H3K36me3 (ab9050), anti-PI3K class 3 (ab124905), and anti-TFEB (ab2636) antibodies were purchased from Abcam. Anti-AMPK (2532), anti-ATG12 (4180), anti-CARM1 (3379 for immunblotting, 12495 for immunoprecipitation and ChIP), anti-LC3 (2775), anti-phospho-AMPKαT172 (2535), anti-phospho-FOXO3a S413 (8174), anti-SQSTM1/p62 (5114), and anti-TFE3 (14779) antibodies were from Cell Signaling Technology. Anti-SKP2 (sc-7164), anti-CUL1 (sc-17775), anti-tubulin (sc-8035), and anti-Lamin A/C (sc-6215) were from Santa Cruz Biotechnology. Anti-Flag (F3165), anti-ULK1 (A7481) and anti-β-actin (A1978) antibodies were from Sigma, anti-HA antibody (MMS-101R) from Covance, and anti-tubulin antibody (LF-PA0146A) from Abfrontier. The following chemicals were used in this study: rapamycin (R-5000) was purchased from LC laboratories, cycloheximide (C4859), AICAR (A9978) and phenformin (P7045) from Sigma, bafilomycin A1 (11038) and ellagic acid from Cayman (10569), compound C from Calbiochem (171260), and MG132 (M-1157) from A.G. Scientific.

Shin H.J., Kim H., Oh S., Lee J.G., Kee M., Ko H.J., Kweon M.N., Won K.J, & Baek S.H. (2016). AMPK–SKP2–CARM1 signalling cascade in transcriptional regulation of autophagy. Nature, 534(7608), 553-557.

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Diverse Antibody and Reagent Sources

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PHF1 (phospho-Ser396/404) and 12E8 (phospho-Ser262) antibodies were kindly provided by Dr. P. Davies and Dr. P. Seubert, respectively. Anti-tau polyclonal (A0024) antibody was purchased from Dako. Anti-Nrf2 (12721), TFEB (4220), mTOR (2983), pmTOR (phospho-Ser2448, 5536), p70S6K (2708), pp70S6K (phospho-Thr389, 9234), 4E-BP1 (9644), p4E-BP1 (phospho-Thr37/46, 2855), NDP52 (9036) and HA (2367) antibodies were purchased from Cell Signaling Technology. Anti-LC3 antibody (PD014) was obtained from Medical & Biological Laboratories. Anti-lamin (A/C) (SC-6215) and β-actin (MAB1501) antibodies were purchased from Santa Cruz Biotechnology and Millipore, respectively. Anti-heme oxygenase (HO)-1 (ADI-SPA-895) and p62/SQSTM1 (BML-PW9860) antibodies were obtained from Enzo Life Sciences. Anti-ATG9b (PA5-20998) antibody was purchased from Thermo Fisher Scientific. Anti-tubulin (T6074) antibody was obtained from Sigma. The GFP-LC3 plasmid was used previously18 (link). The GSK-3β-S9A plasmid was kindly provided by Dr. E. Choi. The mouse TFEB specific predesigned siRNA (S74859) was purchased from Life Technology. The ON-TARGET plus mouse Nrf2 (18024) siRNA SMART pool was purchased from GE Healthcare. Fisetin (5016) was purchased from Tocris. Chemicals such as bafilomycin A1 (B1793) and chloroquine (C6628) were obtained from Sigma.

Kim S., Choi K.J., Cho S.J., Yun S.M., Jeon J.P., Koh Y.H., Song J., Johnson G.V, & Jo C. (2016). Fisetin stimulates autophagic degradation of phosphorylated tau via the activation of TFEB and Nrf2 transcription factors. Scientific Reports, 6, 24933.

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Investigating Wnt Signaling Pathway

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Anti LEF1 (sc374522, Santa Cruz); anti β−catenin (ab32572, AbCam); anti phospho β−catenin (9561, Cell Signaling); anti TG2 (3557, Cell signaling); anti TG2 (sc71632, Santa Cruz); anti Lamin A/C (sc-6215, Santa Cruz); anti Tubulin (T-4026, Sigma); anti Wnt5a (MAB645, R&D); anti Wnt10b (PA5-20530, Invitrogen).

Rossin F., Costa R., Bordi M., D’Eletto M., Occhigrossi L., Farrace M.G., Barlev N., Ciccosanti F., Muccioli S., Chieregato L., Szabo I., Fimia G.M., Piacentini M, & Leanza L. (2021). Transglutaminase Type 2 regulates the Wnt/β-catenin pathway in vertebrates. Cell Death & Disease, 12(3), 249.

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Antibody and Chemical Reagents for Cell Analysis

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The following antibodies were used: anti-Flag (F3165) and anti-β-actin (A1978) (Sigma-Aldrich, St. Louis, MO, USA); anti-GFP (sc-9996) and anti-Lamin A/C (sc-6215) (Santa Cruz biotechnology, Dallas, TX, USA); anti-Tubulin (LF-PA0146A) (AbFrontier, Seoul, Korea); anti-PHF20 (#3934), anti-WDR5 (#13105), and anti-LC3 (#2775) (Cell Signaling Technology, Danvers, MA, USA); anti-HA (#MMS-101R) (Covance, Princeton, NJ, USA); Alexa Fluor 488 donkey anti-rabbit IgG (A21206) and Alexa Fluor 594 donkey anti-mouse IgG (A21203) (Invitrogen, Waltham, MA, USA). The following chemicals were used: hygromycin (H3274), puromycin (P8833), and CQ (C6628) (Sigma-Aldrich, St. Louis, MO, USA); Bafilomycin A1 (#11038) (Cayman, Ann Arbor, MI, USA); and rapamycin (R-5000) (LC laboratories, Woburn, MA, USA).

Park S.W., Kim J., Oh S., Lee J., Cha J., Lee H.S., Kim K.I., Park D, & Baek S.H. (2022). PHF20 is crucial for epigenetic control of starvation-induced autophagy through enhancer activation. Nucleic Acids Research, 50(14), 7856-7872.

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Analyzing NOLC1 and TRF2 in Cancer Cell Lines

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The human embryonic kidney cell line HEK293T, the human breast cancer cell line MCF7, the human hepatoma cell line HepG2, and Hela cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin, and 100 μg/ml streptomycin in 5% CO₂ at 37 °C. The antibodies used in this study were as follows: anti-NOLC1 (ab184550, Abcam, Cambridge, MA, USA; and sc-374033, Santa Cruz Biotechnology, Dallas, TX, USA), anti-TRF2 (ab13579, Abcam), anti-UBF (sc-9131, Santa Cruz Biotechnology), anti-Lamin A/C (sc-6215, Santa Cruz Biotechnology), anti-GAPDH (5632-1, Epitomics, Cambridge, MA, USA), anti-Flag (F1804, Sigma, St Louis, MO, USA), anti-H3 (AM8433, ABGENT, San Diego, CA, USA) and anti-actin (SC-130300, Santa Cruz Biotechnology). The Flag-NOLC1 expression vector was constructed by inserting the full-length NOLC1 cDNA into the pCMV-Flag vector. The GFP-TRF2 expression vector was purchased from Addgene (ID no. 19798).

Yuan F., Li G, & Tong T. (2017). Nucleolar and coiled-body phosphoprotein 1 (NOLC1) regulates the nucleolar retention of TRF2. Cell Death Discovery, 3, 17043-.

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Analysis of NF-κB Inhibition and Golgi Markers

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NF-κB inhibitor BAY 11–7085 (SC-202490, Santa Cruz Biotechnology, Dallas, TX, USA) and monensin (M5273, Sigma Chemical Co., St. Louis, MO, USA) were dissolved in dimethyl sulfoxide (DMSO) and diluted in fresh medium before use. Antibodies used in this study and their sources are as follows: anti-golgin-97 (ab84340; Abcam Inc., Cambridge, MA, USA); anti-E-cadherin (sc-7870), anti-p65 (sc-8008), anti-GAPDH (sc-6215), and anti-Lamin A/C (sc-6215) (Santa Cruz Biotechnology, Dallas, TX, USA); anti-IκB (#4814; Cell Signaling Technology, Beverly, MA, USA); anti-phospho-NF-κB p65 (Ser529) (GTX38622; GeneTex, Irvine, CA, USA); anti-β-actin (Millipore, Bedford, MA, USA); anti-TGN46 (AD Serotec, AHP500; Bio-Rad Laboratories, Inc., Hercules, CA, USA); and anti-GM130 (610823; BD Transduction Laboratories, Lexington, KY, USA). The rabbit polyclonal antibody against human Arl1 was produced in-house as described previously [28 (link)]. Anti-GCC185 was kindly provided by Dr. Fang-Jen S. Lee, National Taiwan University, Taiwan.

Hsu R.M., Zhong C.Y., Wang C.L., Liao W.C., Yang C., Lin S.Y., Lin J.W., Cheng H.Y., Li P.Y, & Yu C.J. (2018). Golgi tethering factor golgin-97 suppresses breast cancer cell invasiveness by modulating NF-κB activity. Cell Communication and Signaling : CCS, 16, 19.

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Antibody Characterization for Pontin Regulation

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The following commercially available antibodies were used: anti-Pontin (SAB4200194), anti-Flag (F3165), and anti-β-actin (A1978) (Sigma-Aldrich); anti-Pontin (sc-15259), anti-GST (sc-459), anti-GFP (sc-9996), and anti-Lamin A/C (sc-6215) (Santa Cruz Biotechnology); anti-Tubulin (LF-PA0146A) (Abfrontier); anti-asymmetric dimethyl arginine motif (Rme2a) (#13522), anti-CARM1 (#3379), anti-LC3 (#2775), anti-FOXO3a (#2497), anti-phospho-FOXO3a S413 (#8174), and anti-Tip60 (#12058) (Cell Signaling Technology); anti-FOXO3a (ab12162) and anti-H3R17me2 (ab8284) (Abcam); anti-acetyl-Histone H4 (06-866) (Millipore); anti-HA (#MMS-101R) (Covance); anti-ATG9A (NB110-56893) (Novus); anti-ATG14 (#PD026) (MBL). Following commercially available fluorescent-labeled secondary antibodies were used: Alexa Fluor 488 donkey anti-rabbit IgG (A21206) and Alexa Fluor 594 donkey anti-mouse IgG (A21203) (Invitrogen). We used antibodies recommended by the manufacturer for the species and application. The antibody specifically recognizing arginine methylated Pontin at R333 and R339 (Pontin-me) was generated by Peptron. Regarding chemicals, hygromycin (H3274), puromycin (P8833), and chloroquine (C6628) were from Sigma-Aldrich. Bafilomycin A1 (#11038) was from Cayman. Rapamycin (R-5000) was from LC Laboratories. EPZ025654 (AOB33871) was from AOBIOUS. EZM2302 (HY-111109) was from MedChemExpress.

Yu Y.S., Shin H.R., Kim D., Baek S.A., Choi S.A., Ahn H., Shamim A., Kim J., Kim I.S., Kim K.K., Won K.J, & Baek S.H. (2020). Pontin arginine methylation by CARM1 is crucial for epigenetic regulation of autophagy. Nature Communications, 11, 6297.

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Immunofluorescence Staining of Myoblasts

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Myoblasts were fixed for 5 min with 4% formaldehyde, permeabilized with 0.5%Triton X100 and blocked with 5% bovine serum albumin (BSA) diluted in PBS. Myoblasts were stained with Phalloidin-Alexa 568 to label F-actin (Interchim, Montluçon, France). The following primary antibodies were used for immunostaining: anti-vinculin (Sigma-Aldrich, Saint Quentin-Fallavier, France), anti-non muscle myosin IIA (NM-2A) (Abcam, Paris, France), anti-lamin A/C (sc-6215, Santa Cruz Biotechnology, Santa Cruz, California, USA), anti-emerin (NCL-emerin Novocastra), and anti-SUN2^{11 (link)} (generously provided by D. Hodzic), anti-nesprin 1 (N1G-7C8 and MANNES1A), which recognize exons 84-85 and exons 143-146 of nesprin-1, respectively)^{54 (link)}, anti-nesprin-2 (MANNES2A)^{54 (link)}, and anti FHOD1 (ab73443, Abcam). Secondary antibodies (Life technologies, Saint-Aubin, France; 1/500) were: Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 568 goat anti-rabbit IgG, Alexa Fluor 488 donkey anti-mouse IgG, or Alexa Fluor 488 rabbit anti-goat IgG. The preparations were mounted on slides with fluorescent mounting medium containing DAPI (Vectashield, Vector Labs, Berlingame, California). Confocal images were taken with an Olympus FV 1000 (Olympus, Hamilton, Bermuda) and a Leica SP2 (Leica Microsystems, Wetzlar, Germany) microscopes.

Schwartz C., Fischer M., Mamchaoui K., Bigot A., Lok T., Verdier C., Duperray A., Michel R., Holt I., Voit T., Quijano-Roy S., Bonne G, & Coirault C. (2017). Lamins and nesprin-1 mediate inside-out mechanical coupling in muscle cell precursors through FHOD1. Scientific Reports, 7, 1253.

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