Uv 3600 plus spectrometer

Diffuse reflectance spectra of the petals (adaxial side) and dehisced anthers with pollen were measured from 300 nm to 700 nm using a UV-3600 Plus spectrometer (Shimadzu, Kyoto, Japan) equipped with an integrating sphere unit ISR-603 (Shimadzu). The samples were measured in a powder sample cell (Shimadzu) using Spectralon (Labsphere, NH, USA) as a white standard. Data were processed with a software UV-Probe 2.50 (Shimadzu) and are presented as the mean of five biological replicates.

The photocatalytic experiments were carried out in a home-made photochemical Reactor equipped with a 300 W Xe lamp. To acquire visible light, a 420 nm cut off filter was applied between the lamp and the catalyst container. Rhodamine B (RhB) was adopted as the photocatalysis probe due to its high stability and sensitivity to visible light absorption due to its intrinsic absorption band at about 553 nm. In each photocatalytic experiment, 50 ml RhB aqueous solution in concentration 10 mg/L was filled into quartz beaker together with 50 mg BiLa_1.4Ca_0.6O_4.2 powder. Before irradiation, the mixture was magnetically stirred in dark for 1.5 hours to approach adsorption-desorption equilibrium. The mixtures were then irradiated by visible light at room temperature and ambient pressure, while stirring to keep catalyst particles homogenously dispersed in solution.
During visible light irradiation, about 5 mL of the suspension was taken out from the beaker at a given time intervals about 2 hours in sequence for subsequent analysis of target dye concentration after centrifuging. Absorption spectra of the suspensions were collected by a Shimadzu UV-3600 Plus spectrometer. The photocatalytic activity of the BiLa_1.4Ca_0.6O_4.2 was evaluated from the intrinsic absorption band (at 553 nm) intensity ratio of the remnant RhB after visible light illumination to that of the RhB in parent solution.

The morphologies of Au nanospheres were investigated using a Gemini SEM 300 scanning electron microscope. The UV–vis absorption spectra was detected using a Shimadzu UV-3600 plus spectrometer (Japan). The electrochemical measurements were conducted by a CHI660E electrochemical workstation (Shanghai Chenhua Co., China) with a conventional three-electrode setup consisting of a platinum wire as an auxiliary electrode, a saturated calomel electrode (SCE) as a reference electrode, and a bare or modified glassy carbon electrode as the working electrode.