Thin sections were cut with a diamond knife on an Ultra 45°C (Daitome AG, Nidau, Switzerland) and examined using a Tecnai G2 20 Twin transmission electron microscope (FEI, Hillsboro, OR, USA). Uranium lead double staining (with 2% uranyl acetate saturated aqueous solution, and then citrate for 15 min each). Ultrastructural features of autophagy in microglial cells were studied by TEM as previously described (25 (link)). Briefly, microglial cells were harvested following exposure to hypoxia, washed with PBS, fixed with 1% glutaraldehyde in 0.1 M cacodylate buffer for 2 h at 4°C, then post-fixed with 1% OsO4 for 1.5 h in the same buffer at 20°C. The samples were then washed, dehydrated, and embedded in 812 embedding medium (SPI Supplies, Inc, West Chester, PA, USA) at 20°C for 12 h. Thin sections (60–80 nm) were cut with a diamond knife on an Ultra 45°C (Daitome AG) and examined using a Tecnai G2 20 Twin transmission electron microscope (FEI, Hillsboro, OR, USA).
Tecnai g2 20 twin transmission electron microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Tecnai G2 20 TWIN transmission electron microscope is a high-performance instrument designed for advanced imaging and analysis of materials at the nanoscale. It features a twin-lens configuration, providing high-resolution imaging capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Vitrobot mark 4, by Thermo Fisher Scientific (2 mentions)
Em uc7 ultramicrotome, by Leica (1 mentions)
Eagle 4k ccd camera, by Thermo Fisher Scientific (1 mentions)
Uc7 fc7, by Leica (1 mentions)
Em uc7 microtome, by Leica (1 mentions)
Su8010 scanning electron microscope, by Hitachi (1 mentions)
Em uc6 microtome, by Leica (1 mentions)
Quanta 200 scanning electron microscope, by Thermo Fisher Scientific (1 mentions)
Ls55 spectrofluorometer, by PerkinElmer (1 mentions)
Lambda 35 uv visible spectrophotometer, by PerkinElmer (1 mentions)
Zetasizer software v7, by Malvern Panalytical (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
51 inverted fluorescence microscope, by Olympus (1 mentions)
26 protocols using tecnai g2 20 twin transmission electron microscope
1
Ultrastructural Analysis of Microglial Autophagy
2
Transmission Electron Microscopy Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Morphological analysis was performed using a Tecnai G2 20 TWIN transmission electron microscope (FEI, Hillsboro, OR, USA) as described in (33 (link)).
3
Transmission Electron Microscopy of hIAPP Aggregates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The images of hIAPP aggregates were recorded by Tecnai G2 20 TWIN transmission electron microscope (FEI, USA) at 200 kV under vacuum. The point resolution was 0.27 nm, and the tracer resolution was 0.14 nm.
Image J software was used to measure the diameters of amyloid fibrils and amorphous aggregates in the TEM images. Mean diameters of fibrils and amorphous aggregates were calculated from 40 randomly selected fibrils or aggregates in each group. Results are expressed as mean ± SD [38 ].
Image J software was used to measure the diameters of amyloid fibrils and amorphous aggregates in the TEM images. Mean diameters of fibrils and amorphous aggregates were calculated from 40 randomly selected fibrils or aggregates in each group. Results are expressed as mean ± SD [38 ].
4
Ultrastructural Analysis of Cell Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell or tumour samples were fixed in 2.5% glutaraldehyde and washed three times in PBS the next day. Samples were then fixed with 1% osmium acid at 4°C for 2‐3 h followed by washing in PBS and dehydration through an alcohol gradient. Successive permeation was then performed using acetone:epoxy resin (2:1), acetone:epoxy resin (1:1) and epoxy resin. Samples were embedded into epoxide resin, sliced with an EM UC7 ultramicrotome (Leica, Germany) and observed with a Tecnai G2 20 TWIN transmission electron microscope (FEI, USA).
5
Visualizing Extracellular Vesicle Surface Markers
6
Ultrathin-sectioning of Tannerella forsythia
7
Protein Sample Preparation for TEM Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5 μL of the protein solution was collected from the solution of ThT measurements and diluted to 25 μg/mL solution with Tris buffer quickly. Then, 10 μL of the diluted sample was blotted on a Cu(II) grid (carbon-coated Formvar 300 mesh, Electron Microscopy Sciences, Hatfield, PA, USA), and stained with 2% (w/v) uranyl acetate for 20 min. The grids were allowed to dry thoroughly. Images were acquired using a Tecnai G2 20 TWIN transmission electron microscope (FEI, Hillsboro, OR, USA) at 200 kV.
8
Ultrastructural Analysis of ADSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On the 15th day of the co‐culture, ADSCs were harvested and centrifuged, and then were fixed in 2.5% glutaric dialdehyde (Boster Biotech). After incubation at 4°C for 12 hrs, the cells were fixed in 1% osmic acid (Boster Biotech) at 4°C for 1 hr, dehydrated in graded acetone series, and embedded in Epon 812 (Boster Biotech). Ultrastructural identification was performed under Tecnai G220TWIN transmission electron microscope (Fei, Hillsboro, OR, USA).
9
Baicalin Modulates PAVECs-Haemophilus Interaction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The effect of baicalin on the interaction between PAVECs and H.
parasuis was examined by transmission electron microscopy (TEM) as
described previously, with minor modifications.16 (link) PAVECs (2 × 107) were seeded onto 24-well plates and treated
with baicalin at a concentration of 12.5, 25, 50, or 100 μg/ml for 2 h prior to
bacterial infection. H. parasuis (2 × 107 CFU/ml)
was added to the plates and co-incubated for 12 h. The infected cells were
gently washed five times with PBS. PAVECs were fixed with 0.1 M cacodylate
buffer (pH 7) including 5% glutaraldehyde and 0.15% ruthenium red at 37°C for
5 h. PAVECs were reacted with polycationic ferritin (1 mg/ml). The thin sections
were examined by a Tecnai G2 20 TWIN transmission electron microscope
(FEI, Hillsboro, OR).
parasuis was examined by transmission electron microscopy (TEM) as
described previously, with minor modifications.16 (link) PAVECs (2 × 107) were seeded onto 24-well plates and treated
with baicalin at a concentration of 12.5, 25, 50, or 100 μg/ml for 2 h prior to
bacterial infection. H. parasuis (2 × 107 CFU/ml)
was added to the plates and co-incubated for 12 h. The infected cells were
gently washed five times with PBS. PAVECs were fixed with 0.1 M cacodylate
buffer (pH 7) including 5% glutaraldehyde and 0.15% ruthenium red at 37°C for
5 h. PAVECs were reacted with polycationic ferritin (1 mg/ml). The thin sections
were examined by a Tecnai G2 20 TWIN transmission electron microscope
(FEI, Hillsboro, OR).
10
Ultrastructural Identification of Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells in P3 were harvested and centrifuged, and then were fixed in 2.5% glutaric dialdehyde (Boster Biotech, Wuhan, China). After incubation at 4°C for 12 h, the cells were fixed in 1% osmic acid (Boster Biotech, Wuhan, China) at 4°C for 1 h, dehydrated in graded acetone series, and embedded in Epon 812 (Boster Biotech, Wuhan, China). Ultrastructural identification was performed under Tecnai G220TWIN transmission electron microscope (Fei, Hillsboro, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!