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9 protocols using anhydrous ethanol

1

Electrochromic Device Fabrication

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The dispersion of WO3 particles in ethanol (ECS-C1) was purchased from Adchro Inc. PEO with molecular weights (Mw) of 100,000 and 600,000, anhydrous propylene carbonate (PC, 99.7%), and ferrocene (Fc, 98%) were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Lithium perchlorate anhydrous (LiClO4) was purchased from Wako Pure Chemical Corporation (Osaka, Japan), and anhydrous ethanol (99.9%) was purchased from Daejung Chemicals (Seoul, Korea) and Metals and used without additional purification. For assembling the ECD, we used indium-tin oxide (ITO)-coated glass (10 Ω, AMG Tech), ITO-coated poly(ethylene terephthalate) (PET (10 Ω, WJ Chemical) and Surlyn (60 µm, SOLARONIX). Conventional polypropylene (PP) nonwoven fabric was used. Polyurethane was purchased from Hepce Chemical, and silver-coated polyamide/polyester hybrid thread (85 Ω/m, Silver-tech) was used as a conductive thread.
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2

Synthesis and Characterization of NIC Compound

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NIC (C13H8Cl2N2O4) was obtained from DERIVADOS QUIMICOS, Murcia, Spain. Anhydrous Ethanol (99.9 %), and isopropyl alcohol (IPA) were purchased from Daejung, Gyeonggi-do, South Korea. BSA and (β-cyclodextrin were purchased from Sigma Aldrich, USA. PEG, weight average molecular weight, Mn ∼ 10,000, was purchased from Sigma, Milwaukee W1 53233, USA.
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3

Synthesis of Bimetallic Electrocatalysts

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Carbon cloth (CC, HCP331; 0.35 +/−0.02 mm; WizMAC Co. Ltd.), Co(NO3)2·6H2O (≥98%, ACS reagent; Sigma‐Aldrich), FeSO4·7H2O (≥99%; Sigma‐Aldrich), hydrazine monohydrate (80% Assay; Daejung Chemicals), KOH (85% Assay, Extreme pure; Reagents Duksan), anhydrous ethanol (99.5%; Daejung Chemicals), Na2PdCl4 (98%; Sigma‐Aldrich), NaCl (≥99% ACS reagent; Sigma‐Aldrich), Pt—C (20 wt% on carbon black; Alfa Aesar). All chemicals were used as received unless a specific process or purification was mentioned separately.
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4

PVDF Film Preparation and Modification

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PVDF film (thickness; 0.08 mm) was purchased from Fils Co., Ltd. (Seongnam, Republic of Korea), and was ultrasonicated in water and washed with ethanol prior to use. Anhydrous ethanol (99.9%, Daejung, Siheung, Republic of Korea), sodium hydroxide (guaranteed reagent, JUNSEI, Tokyo, Japan), pyrrole (reagent grade; 98%, Sigma Aldrich, St. Louis, MO, USA), anthraquinone-2-sulfonic acid sodium salt monohydrate (AQSA-Na, 97%, Sigma Aldrich, St. Louis, MO, USA), FeCl3·6H2O (reagent grade; ≥98%, Sigma Aldrich, St. Louis, MO, USA), and DTMS (>93%, Tokyo Chemical Industry Co., Ltd., Tokyo, Japan) were used as received without any further purification.
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5

Synthesis of ZnO Nanoparticles

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Pellet-type PEICT (ECOZEN T95) (Mw = 65,400 g/mol) was kindly provided by SK Chemicals (Korea). Chloroform (99%) and trifluoroacetic acid (TFA, 98%) were purchased from Wako Chemicals (Japan). Zinc acetate dehydrate (Zn(CH3COO)2∙2H2O, Sigma-Aldrich, Milwaukee, WI, USA), potassium hydroxide (KOH, Junsei Chemical Co. Ltd., Tokyo, Japan), anhydrous ethanol (99.9%, Daejung Chemicals & Metals Co. Ltd., Siheung, Korea), methanol (CH3OH, OCI Co. Ltd., Seoul, Korea), and n-butanol (Sigma-Aldrich, Milwaukee, WI, USA) were used to synthesize the ZnO nanoparticles. The reagents were used without further purification, or as otherwise noted.
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6

Synthesis and Characterization of NIC Nanoparticles

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NIC (C13H8Cl2N2O4) was purchased from Derivados Quimicos, Murcia, Spain. Anhydrous Ethanol (99.9%) and isopropyl alcohol (IPA) were purchased from Daejung, Gyeonggi-do, South Korea. Magnesium nitrate hexahydrate [Mg(NO3)2·6H2O], aluminum nitrate nonahydrate [Al(NO3)3·9H2O], sodium hydroxide (NaOH), and formamide (CH3NO) were obtained from Daejung Co. Ltd., Gyeonggi-do, South Korea. ES100 was purchased from EVONIK (Darmstadt, Germany) and Tween 60 was purchased from TCI (Tokyo, Japan).
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7

Synthesis of Gold Nanoparticles Using CTAB

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Cetyltrimethylammonium bromide (CTAB, ≥99.9%), l-ascorbic acid (AA, 100%), sodium borohydride (NaBH4, ≥99%), trisodium citrate dihydrate (Na3C6H5O7·2H2O, Na3Cit), gold chloride hydrate (HAuCl4·xH2O, 99.999%) and poly(ethylene glycol) methyl ether thiol (thiolated polyethylene glycol, PEG–SH, average Mn = 2000) were obtained from Sigma-Aldrich. Cetyltrimethylammonium chloride (CTAC, ≥98%) was obtained from Wako. Sodium hydroxide (NaOH, 1 M), anhydrous ethanol (C2H5OH ≥ 99.9%) and dichloromethane (CH2Cl2 ≥99.5%) were obtained from Daejung. 3-Mercaptopropionic acid (MPA ≥ 99%) was purchased from Alfa Aesar. Si wafers and wet oxidized (300 nm) SiO2/Si wafers were purchased from DASOM RMS. These chemicals were used without further purification with deionized water (Pure Water Corporation). Fluorine-doped tin oxide (FTO, 8 Ω □−1) was purchased from Wooyang GMS. Titanium dioxide (TiO2), tungsten oxide (WO3) and hematite (α-Fe2O3) were purchased from Kojundo Chemical (grain shape, 99.9%).
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8

Cobalt-based Catalytic Ethanolysis

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The anhydrous ethanol (CH 3 CH 2 OH, 99.5%, M w = 46.07), Triton X-100 (t-Oct-C 6 H 4 -(OCH 2 CH 2 ) x OH, M w = 625) and potassium hydroxide (KOH, 85%, M w = 56.11) were purchased from Daejung, Korea. Cobalt(II) chloride hexahydrate (CoCl 2 •6H 2 O, 98%, M w = 129.8) was obtained from Junsei, Japan. CNF was donated by Moorim P&P resided in Ulsan, Korea.
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9

Zinc-Based Inorganic Complexes Synthesis

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All reagents were used as received
without further purification. Zinc chloride (>98%, ZnCl2) and zinc nitrate (>99%, Zn(NO3)2(H2O)6) were purchased from Merck KGaA (Darmstadt,
Germany).
DAT (>98%, C2H5N5) was purchased
from Acros Organics (Geel, Belgium), and anhydrous ethanol (99.9%)
was purchased from Dae Jung Chemicals & Metals (Seoul, Korea).
HeLa, NCCIT, and MCF-7 cell lines were purchased from the American
Type Culture Collection (Manassas, Virginia).
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