Equal amounts of whole-cell lysate were loaded onto a 10% or 12% sodium dodecyl sulfate-polyacrylamide gel and assayed by enhancing the chemiluminescence as described by the manufacturer (PerkinElmer Inc., Waltham, MA, USA). The blotting membranes were probed with antiserum of NIK, IĸBα, Akt, phospho-AktS473, Slug (Cell Signaling Technology, Danvers, MA, USA), MMP9, Snail (Abcam, Cambridge, MA, USA), MIEN1 (OriGene Technologies), NDRG1 (Invitrogen Thermo Fisher Scientific Inc., Waltham, MA, USA), E-cadherin (Santa Cruz Biotechnology, Santa Cruz, CA, USA), N-cadherin (Abgent, San Diego, CA, USA), or β-actin (Merck Millipore, Burlington, MA, USA). The intensities of different bands were analyzed using the GeneTools of ChemiGenius (Syngene, Cambridge, UK).
N cadherin
Manufactured by Abcepta
Sourced in United States
N-cadherin is a type of cell adhesion molecule that plays a crucial role in the formation and maintenance of tissues and organs. It is a transmembrane protein that facilitates the adhesion between cells, allowing them to communicate and coordinate their activities. N-cadherin is essential for the development and function of various tissues, including the nervous system, cardiovascular system, and musculoskeletal system.
Automatically generated - may contain errors
Lab products found in correlation
Vimentin, by Abcepta (3 mentions)
β actin, by Merck Group (3 mentions)
Snail, by Abcam (2 mentions)
Western lightning plus ecl detection system, by PerkinElmer (2 mentions)
E cadherin, by Abcepta (2 mentions)
Phospho akt s473, by Cell Signaling Technology (1 mentions)
Genetools of chemigenius, by Syngene (1 mentions)
Mmp 9, by Abcam (1 mentions)
E cadherin, by Santa Cruz Biotechnology (1 mentions)
Phospho sapk jnk, by Cell Signaling Technology (1 mentions)
Ndrg1, by Thermo Fisher Scientific (1 mentions)
Phospho stat3, by Merck Group (1 mentions)
Chemigenius image capture system, by Syngene (1 mentions)
Phospho p44 42 mapk, by Cell Signaling Technology (1 mentions)
P44 42 mapk, by Cell Signaling Technology (1 mentions)
P38 mapk, by Cell Signaling Technology (1 mentions)
Phospho p38 mapk, by Cell Signaling Technology (1 mentions)
Celecoxib, by Merck Group (1 mentions)
Cdc25a, by Cell Signaling Technology (1 mentions)
P raf, by Cell Signaling Technology (1 mentions)
5 protocols using n cadherin
1
Immunoblotting Analysis of EMT Markers
2
Western Blot Analysis of EMT Markers
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Equal quantities of cell extracts were separated on a 10% SDS-PAGE gel, transferred, and analyzed by the Western lightning plus-ECL detection system (Perkin Elmer, Inc., Waltham, MA, USA). Briefly, antibodies against NDRG1, NDRG2, NDRG3 (Invitrogen), Snail (Abcam, Cambridge, UK), cyclin E, E-cadherin, STAT3, N-cadherin, Vimentin (Abgent, San Diego, CA, USA), Slug, p44/42 MAPK, Phospho-p44/42 MAPK, SAPK/JNK, Phospho-SAPK/JNK, p38 MAPK, Phospho-p38 MAPK (Cell Signaling, Danvers, MA, USA), Phospho-STAT3 and β-actin (Millipore) were used and the protein bands were detected and quantified with the ChemiGenius Image Capture System (Syngene, Cambridge, UK) and the GeneTools Program of ChemiGenius (Syngene).
3
Comprehensive Molecular Signaling Profiling
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A549 and H1299 cells were purchased from the American Type Culture Collection (ATCC, Philadelphia, PA, USA). Antibodies used for WB are listed as following: β-actin (Abgent, San Diego, USA), N-Cadherin, p-FAK, FAK, MMP-9, Cleaved PARP, Cleaved caspase-9, Cleaved caspase-8, Cleaved caspase-7, Cleaved caspase-3, Bcl-2, Bcl-xl, Bad, Bax, Cyto-c, γ-H2AX, p53, p21, Cdc25A, p-Raf, Raf, p-MEK, MEK, p-ERK, ERK, p-PI3P, PI3P, p-AKT, AKT (Cell Signaling Technology, Beverly, MA, USA), p-ATM, p-CHK2, CDK2 (Abcam Inc., Cambridge, MA). Celecoxib(≥98%,HPLC), metformin(purity: > 99.9%), and pifithrin-α were purchased from Sigma (St. Louis, USA).
4
Immunoblotting Analysis of Signaling Proteins
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Cells were lysed in RIPA buffer (50 mM Tris-Cl at pH 7.5, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 1 mM EDTA, 1 μg/mL leupeptin, 1 μg/mL aprotinin, 0.2 mM PMSF) and proteins (20–40 μg) were separated on 8–10% SDS/PAGE gel and then transferred onto PVDF membranes (Millipore, Billerica, MA). After blocking procedure, membranes were incubated with primary antibodies (1:1000), HRP-conjugated secondary antibodies (1:5000), and visualized in Imager (Bio-Rad) using ECL system (Thermo Fisher Scientific, Rochester, NY). Antibodies of ATM, p-ATM, JAK1, p-JAK1, JAK2, p-JAK2, STAT3, and p-STAT3, were from Gene Tex (Irvine, CA), Antibodies of E-cadherin, N-cadherin, Vimentin, Snail, Zeb1, Twist and VEGF were obtained from Abgent (San Diego, CA) and antibodies of PD-L1, GAPDH, were from Cell Signaling (Danvers, MA).
5
Western Blot Analysis of Protein Expression
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Equal quantities of cell extracts which was measured by BCA protein assay kit were separated onto a 10% SDS-PAGE gel, transferred and analyzed by the Western lightning plus-ECL detection system (Perkin Elmer, Inc., Waltham, MA). Antibodies against HO (HO-1; Hsp32, Stressgen, Victoria, BC, Canada), PARP, cleaved PARP (BD Biosciences), N-cadherin, Vimentin (Abgent, San Diego, CA), Lamin B1 (Santa Cruz Biotechnology, Santa Cruz, CA), Slug, and β-actin (Millipore, Temecula, CA) were used.
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