Mouse monoclonal anti ha tag

The antibodies with their dilutions and sources were as follows: antibodies for immunofluorescence: mouse monoclonal anti‐HA‐tag [1 : 200; Cell Signaling Technologies (CST; Danvers, MA, USA)], rabbit polyclonal anti‐calnexin (CANX, 1 : 200; Santa Cruz Biotechnology, Dallas, TX, USA), Alexa Fluor 568‐goat anti‐mouse IgG (1 : 200; Molecular Probes, Eugene, OR, USA), Alexa Fluor 647‐goat anti‐rabbit IgG (1 : 200; Molecular Probes). Antibodies for western blotting: rabbit polyclonal anti‐HA (1 : 4000; Sigma‐Aldrich, St. Louis, MO, USA), anti‐Histone H3(1 : 1000, CST), rabbit polyclonal anti‐GAPDH (1 : 2500; Abcam, Cambridge, UK), mouse monoclonal anti‐α‐tubulin (1 : 10 000; Sigma‐Aldrich), rabbit anti‐ CANX (1 : 1000; CST), rabbit anti‐BiP (1 : 1000, CST), rabbit anti‐GRP94 (1 : 1000, CST), mouse anti‐ERP72 (1 : 200; Santa Cruz Biotechnology), goat anti‐SEL1L (1 : 200; Santa Cruz Biotechnology), rabbit anti‐HRD1 (1 : 500; CST), rabbit anti‐OS‐9 (1 : 500; Abcam), goat anti‐rabbit IgG‐peroxidase (1 : 50 000; Sigma‐Aldrich), and rabbit anti‐mouse IgG‐peroxidase (1 : 80 000; Sigma‐Aldrich).

Antibodies for Western blot analysis: rabbit monoclonal Anti-HA-tag (Cell Signaling Technology, at 1:1,000 dilution), Rabbit monoclonal anti-SYVN1 (Cell Signaling Technology, at 1:1,000 dilution), mouse monoclonal anti-GAPDH (Abcam, at 1:2,500 dilution) rabbit monoclonal Anti-HA (Cell Signaling, at 1:1,000 dilution), mouse monoclonal anti-RFP (Thermo Fisher Scientific, at 1:1,000 dilution), rabbit anti- Endoglin P3D1 (Santa Cruz Biotechnology, at 1:200 dilution), anti-Mouse IgG Peroxidase antibody (Sigma Aldrich, at 1:40,000 dilution), and anti-Rabbit IgG Peroxidase antibody (Sigma Aldrich, at 1:30,000 dilution).
Antibodies for immunofluorescence: mouse monoclonal anti-HA-tag (Cell signaling Technology, at 1: 200 dilution), rabbit polyclonal anti-calnexin (Santa Cruz Biotechnology, at 1 : 200 dilution), rabbit anti-Histone-H3 (Cell Signaling Technology, at 1:1,000 dilution), mouse monoclonal anti-HA (Cell Signaling Technology, at 1: 200 dilution), Alexa Fluor 568-goat anti-mouse IgG (Molecular Probes, at1:200 dilution), and Alexa Fluor 488-goat anti rabbit IgG (Molecular Probes, at 1:200dilution).