4 hydroxy 2 2 6 6 tetramethylpiperidin 1 oxyl tempol

Sodium thiosulfate, sodium sulfate anhydrate, sodium chlorite (purity: 80%), sodium hypochlorite, hydrochloric acid, sulfuric acid, potassium iodide, and N,N-diethyl-p-phenylenediamine (DPD) were purchased from FUJIFILM Wako Pure Chemical Co. Ltd. Starch was purchased from Kanto Chemical Co., Inc. 4-Hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL) was purchased from Sigma-Aldrich.

5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was purchased from Dojindo Laboratories, Ltd. (Kumamoto, Japan). 4-Hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL) and caffeine were purchased from Sigma-Aldrich (St. Louis, MO). Deionized water (deionization by the Milli-Q system, Merck Millipore, Billerica, MA) was used for all sample preparations.

3-aminophthalhydrazide (luminol), 1-(p-hydroxyphenyl)imidazole (HPI), disodium sulfide (Na₂S), erastin, RSL3, cumene hydroperoxide (CHP), liproxstatin-1, 7-(diethylamino)coumarin-3-carbohydrazide (CHH), 4-hydroxytamoxifen (Tam), monobromobimane (MBB), cysteine (Cys), 3-mercaptopyruvate (3MP), cytochrome c (cyt c), glutathione reductase (GR), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), S-nitrosoglutathione (GSNO), ferrocene, 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL) and isopropyl β-d-1-thiogalactopyranoside (IPTG) were obtained from Sigma-Aldrich. BODIPY C11, diallyl tetrasulfide (DATS) and Amplex Red were obtained from Thermo Fisher Scientific. DEPMPO was obtained from Enzo. H₂O₂ was obtained from Roth. GSH and 5-aminolevulinic acid hydrochloride were obtained from Merck. Sodium disulfide (Na₂S₂), sodium tetrasulfide (Na₂S₄) and 3′,6′-di(O-thiosalicyl)fluorescein (SSP4) were obtained from Dojindo. The synthesis of GSSSG, GS³⁴SSG and GSSSSG was performed as described previously⁴³ . CSSSC was synthesized as described below.