The CCK-8 reagent was purchased from APExBIO (USA). The 2×103 cells were seeded into 96-well plates in advance. The test was started 24 hours later and lasted for 6 consecutive days (0, 1, 2, 3, 4, 5, 6 days). The medium was absorbed and discarded, and CCK-8 reagent was mixed with serum-free medium in a 5ml eppendorf tube (CCK8 reagent: Serum-free medium =10 μL: 90 μL per well) and added into 96-well plates with 100 μL per well. OD value at 450 wavelength was detected on a microplate after incubation at 37°C for 1h.
Wei C., Wang B., Peng D., Zhang X., Li Z., Luo L., He Y., Liang H., Du X., Li S., Zhang S., Zhang Z., Han L, & Zhang J. (2022). Pan-Cancer Analysis Shows That ALKBH5 Is a Potential Prognostic and Immunotherapeutic Biomarker for Multiple Cancer Types Including Gliomas. Frontiers in Immunology, 13, 849592.
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