Cw0159s

Manufactured by CWBIO

Sourced in China

The CW0159S is a laboratory equipment product offered by CWBIO. It serves as a core function for various laboratory applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Further information about the intended use or specific features of this product is not available.

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Lab products found in correlation

Cw0114s, by CWBIO (2 mentions) Cw0113s, by CWBIO (1 mentions) Ar0009, by Boster Bio (1 mentions) T8200, by Solarbio (1 mentions) Ar1176, by Boster Bio (1 mentions) Ab53226, by Abcam (1 mentions) Ab5694, by Abcam (1 mentions)

2 protocols using cw0159s

Immunofluorescent Labeling of Brain Cell Types

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The brain-like tissues were fixed with 4% paraformaldehyde for an hour and washed with 1× phosphate-buffered saline (PBS) 3 times at 5-min intervals. After being incubated in 1× PBS with 5% goat serum (AR0009, Boster, USA), a primary antibody diluted 1:200 and 0.3% Triton X-100 (T8200, Solarbio) overnight at 4 °C, the tissues were washed with 1× PBS 6 times at 10-min intervals and then incubated in 1× PBS with 5% goat serum, a secondary antibody diluted 1:200 and 0.3% Triton X-100 for 4 h before DAPI (AR1176, Boster) was added for an additional 10 min. Finally, the tissues were washed 6 times in 1× PBS at 10-min intervals and observed under an LSCM. All antibodies used are summarised in Table 2. The LSCM software NIS-elements AR was used for measuring and counting the length and the number of axons and dendrites.Table 2

Summary of antibodies used in this study

Cell type	Primary antibody	Secondary antibody
Neurons	Rabbit anti-MAP2 (8707S, CST, USA)	Goat anti-rabbit IgG, Cy3-conjugated (CW0159S, CWBIO, China)
Neurons	Anti-neurofilament (2838S, CST)	Goat anti-mouse IgG, Cy3-conjugated (CW0145S, CWBIO)
Astrocytes	Mouse anti-GFAP (3670S, CST)	Goat anti-mouse IgG, FITC-conjugated (CW0113S, CWBIO)
Astrocytes	Rabbit anti-GFAP (16825-1-AP, Proteintech, USA)	Goat anti-rabbit IgG, FITC-conjugated (CW0114S, CWBIO)

Fang A., Li D., Hao Z., Wang L., Pan B., Gao L., Qu X, & He J. (2019). Effects of astrocyte on neuronal outgrowth in a layered 3D structure. BioMedical Engineering OnLine, 18, 74.

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Immunofluorescence Analysis of TGF-β2 Induced EMT

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The cells (1×10⁵ cells/well) were seeded on an 8×8 mm cover slip in 24-well plates, starved overnight and then treated with 3 ng/ml TGF-β2. Following 48 h of treatment, the cells were fixed with 4% paraformaldehyde for 20 min and washed 3 times with PBS. The cells were permeabilized in PBS with 0.3% Triton X-100 for 5 min. The cells were then blocked with 5% normal goat serum for 1 h. Primary rabbit anti-human α-SMA antibody (1:500, ab5694) and rabbit anti-human E-cadherin antibody (1:1,000, ab53226) (both from Abcam, Cambridge, MA, USA) were incubated with the cells overnight at 4°C. After being rinsed extensively, the cells were exposed to the fluorescent secondary antibody (goat anti-rabbit Cy3, 1:10,000, CW0114S; goat anti-rabbit FITC, 1:10,000, CW0159S; both from CWBIO, Beijing, China) in the dark for 30 min. The coverslips were rinsed again with PBS 3 times and mounted with DAPI for 5 min, as a nuclear counterstain. Finally, the cells were observed under a fluorescence microscope (DP71; Olympus, Tokyo, Japan).

Xing Y., Cui L, & Kang Q. (2016). Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-β2. International Journal of Molecular Medicine, 38(2), 407-416.

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