Human peripheral PBMC from citrated (0.38%) peripheral blood of healthy volunteers were isolated by sedimentation on ficoll hypaque (Amersham, Freiburg, Germany). PBMC were incubated with 8 μg/ml mouse anti‐CXCR6‐phycoerythrin (R&D Systems) or the respective IgG2b isotype control (R&D Systems) in PBS supplemented with 0.2% bovine serum albumin for 1 hr at 4°C and subjected to FACS analysis (LSR II Fortessa; BD Bioscience, Heidelberg, Germany).
Igg2b isotype control
Manufactured by R&D Systems
Sourced in Germany
IgG2b isotype control is a laboratory reagent used as a control in various immunoassays and cell-based experiments. It provides a reference point for evaluating the specificity of antibody binding in the studied system.
Automatically generated - may contain errors
Lab products found in correlation
Ficoll hypaque, by Cytiva (1 mentions)
Lsrii fortessa, by BD (1 mentions)
Anti cd127, by BioLegend (1 mentions)
Anti tnf α, by BioLegend (1 mentions)
Anti human cd3, by BD (1 mentions)
Anti il 10, by BioLegend (1 mentions)
Anti il 17a, by BioLegend (1 mentions)
Anti foxp3, by BioLegend (1 mentions)
Anti cd11c, by BioLegend (1 mentions)
7 aad staining, by BioLegend (1 mentions)
Anti siglec 8, by BioLegend (1 mentions)
Anti cd3, by BioLegend (1 mentions)
Anti il 22, by BioLegend (1 mentions)
Lsrii fortessa flow cytometer, by BD (1 mentions)
Anti cd4, by BioLegend (1 mentions)
Anti ifn γ, by BioLegend (1 mentions)
2 protocols using igg2b isotype control
1
Quantification of CXCR6+ PBMCs
2
Multiparameter Flow Cytometry of Esophageal Immune Cells
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Human fluorochrome-conjugated antibodies, anti-CD127, anti-CD4, anti-CD3, anti-CD11c, anti-Siglec-8, anti-IL17A, anti-IFNγ, anti-TNF-α, anti-IL22, anti-FOXP3, anti-IL10 all were purchased from BioLegend. Additional anti-human CD3 and anti-human CD4 were used from BD Biosciences. Anti-human IL-22BP antibody (clone 87554) and IgG2B isotype control were obtained from R&D Systems. To identify dead cells, 7-AAD staining (BioLegend) was performed. For extracellular staining, isolated hematopoietic cells from esophageal tissues were incubated for 20 min at 4 °C. Cells were acquired on a LSRII Fortessa flow cytometer (BD). Data were analyzed with FlowJo software (Treestar).
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