Nci h82

Human small cell carcinoma cell lines (NCI-H69; ATCC#HTB-119 and NCI-H82; ATCC#HTB-171) and human adenocarcinoma cell lines (PC9; RIKEN BioResource Center#RCB4455) were originally purchased from ATCC or RIKEN BioResource Center and stocked at our institution. The CAFs were obtained from surgically resected small cell carcinoma specimens (Supplementary Table 1), and were cultured according to a previously described method. [28 (link), 29 (link)] NCI-H69 and NCI-H82 were cultured in RPMI1640 (SIGMA-Aldrich, MO) containing 10% fetal bovine serum (FBS; Nichirei Bioscience, Japan) and 1% penicillin and streptomycin (SIGMA-Aldrich). The CAFs were cultured in MEM alpha (Life Technologies Corporation [Gibco], CA) supplemented with 10% FBS and 1% penicillin and streptomycin.

The human SCLC cell line SBC3 was gifted by Dr. Takashi Kijima (Osaka University, Japan), and SBC5 was obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan. BEAS-2B, DMS273, DMS53, NCI-H82, NCI-H526, NCI-H69 and Colo668 were originally obtained from ATCC (Rockville, MD, USA). All the cell lines were routinely maintained and cultured in standard culture conditions, in a CO₂ incubator at 37 °C, and regularly validated using STR profiling and checked for mycoplasma infections prior to experiments. For routine maintenance, SBC3, SBC5, NCI-H82, NCI-H69, NCI-H526, DMS273, DMS53, and Colo668 cells were cultured in RPMI-1640 medium supplemented with 1500 mg/L sodium bicarbonate, 10% fetal bovine serum (Sigma Cat#12303C), 1% penicillin/streptomycin cocktail solution (Invitrogen Cat#15,140–122), 1% sodium pyruvate (Invitrogen Cat#11,360,070), and 1% L-glutamine (Invitrogen Cat#25,030–081).