Quickplex sq instrument

Manufactured by Mesoscale

The QuickPlex SQ instrument is a multiplex assay platform designed for the quantitative measurement of multiple analytes in a single sample. It employs electrochemiluminescence technology to enable sensitive and accurate detection of a wide range of biomolecules, including proteins, peptides, and small molecules. The instrument provides a streamlined and automated workflow, allowing for efficient data generation and analysis.

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QuickPlex SQ 120 Instrument (12 citations) MESO QUICKPLEX SQ 120 instrument (10 citations)

2 protocols using quickplex sq instrument

Circulating Cytokine and Chemokine Analysis

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Concentrations of circulating cytokines and chemokines (IL-1β, IL-6, TNFα, CXCL1 [KC/GRO]) were measured using customized 4-plex fluorescent immunoassays (Meso Scale Discovery, Rockville, MD, USA) chosen based on their association with chemotherapy and behavioral comorbidities and run according to the manufacturer’s instructions. Plasma samples (n = 10–20/group) were diluted two-fold, run in duplicate, and analyzed using a QuickPlex SQ instrument (Meso Scale Discovery). Values below detection limits were reported as a concentration of 0 pg/ml (IL-1β: n = 3/20 vehicle, n = 4/19 chemo; IL-6: n = 4/20 vehicle, n = 5/19 chemo). Intra-assay variation was <10% for Il-1β, IL-2, Il-6, and CXCL1 and <15% for TNFα.

Loman B.R., Jordan K.R., Haynes B., Bailey M.T, & Pyter L.M. (2019). Chemotherapy-induced neuroinflammation is associated with disrupted colonic and bacterial homeostasis in female mice. Scientific Reports, 9, 16490.

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Cytokine Profiling in Breast Cancer

Cited 4 times

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Based on our previous studies and studies of breast cancer patients, protein concentrations of circulating (Experiment 1 and Experiment 3) and hippocampal, hypothalamic, and frontal cortex (Experiment 2) cytokines and chemokines (IL-1β, IL-2, IL-6, TNFα, CXCL1 [KC/GRO]) were measured using multi-plex fluorescent immunoassays (Meso Scale Discovery, Rockville, MD, USA) and run according to the manufacturer’s instructions (Sullivan et al., 2021 (link); Loman et al., 2019 (link); Bagnall-Moreau et al., 2019 (link)). Proinflammatory cytokines, including these, are associated with fatigue and anxiety (DeSanctis, V. et al., 2014 (link); Perez-Tejada, J. et al., 2021 (link)). Samples were run in duplicate and analyzed using a QuickPlex SQ instrument (Meso Scale Discovery). Values below detection limits were reported as the lowest value on the standard curve. This occurred only in Experiment 3 in GMT recipients; IL-1β = 0.49, 6/7 vehicle, 9/9 chemo, IL-6 = 0.61, 2/7 vehicle, 3/9 chemo. Due to a technical error in creating the standard curve on one plate, the sample size for brain regions associated with Experiment 2 are limited. Intra-assay variation was <20%.

Grant C.V., Loman B.R., Bailey M.T, & Pyter L.M. (2021). Manipulations of the gut microbiome alter chemotherapy-induced inflammation and behavioral side effects in female mice. Brain, behavior, and immunity, 95, 401-412.

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