Tertiary butanol

Manufactured by Merck Group

Sourced in China

Tertiary butanol is a colorless, flammable liquid chemical compound. It is commonly used as a solvent and intermediate in various industrial processes.

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Butanol (82 citations)

4 protocols using tertiary butanol

Antimicrobial Susceptibility Assay Protocol

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Mueller-Hinton broth (MHB), Mueller-Hinton agar (MHA) and MRS powder were obtained from AoBoX (China). Bovine serum albumin (BSA), Triton X-100, polymyxin B, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), dimethyl sulfoxide (DMSO), BODIPY-TR-cadaverine (BC), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 3,3′-dipropylthiadicarbocyanine (disc₃_–₅), ethanol (analytical grade, 99%), tertiary butanol (analytical grade, 99%), acetone (analytical grade, 99%), glutaraldehyde (synthetic grade, 50% in H₂O), lipoteichoic acid (LTA) from S. aureus and lipopolysaccharide (LPS) from E. coli were purchased from Sigma-Aldrich (China). Phosphate-buffered saline (PBS) solution, sodium chloride, potassium chloride, ammonium chloride, calcium chloride, zinc chloride, magnesium chloride, and ferric chloride were purchased from Kermel (China). Glucose (analytical grade) was obtained from Zhiyuan (Guangdong, China). DMEM phenol red-free medium and fetal bovine serum were purchased from Gibco (Beijing, China). All these reagents were used according to the required concentration range.

Chou S., Li Q., Nina Z., Shang L., Li J., Li J., Wang Z, & Shan A. (2020). Peptides With Triplet-Tryptophan-Pivot Promoted Pathogenic Bacteria Membrane Defects. Frontiers in Microbiology, 11, 537.

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CD Spectroscopy of Antimicrobial Agents

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Phosphate-buffered saline (PBS) solution obtained from Kermel (Tianjin, China), sodium dodecyl sulfate (SDS) obtained from Sigma-Aldrich (Shanghai, China) and trifluoroEthanol (TFE) purchased from Amresco (Solon, OH, USA) were used for CD as dilution agents. Mueller-Hinton Agar (MHA) powder and Mueller-Hinton Broth (MHB) powder were obtained from AoBoX (Beijing, China) to incubate the bacteria. Ethanol, acetone, and tertiary butanol which were analytical grade (>99%) were all purchased from Sigma-Aldrich (Shanghai, China). Triton X-100, N-phenyl-1-naphthylamine (NPN), HEPES, and glutaraldehyde (synthetic grade, 50% in H₂O) were obtained from Sigma-Aldrich (Shanghai, China).

Tan T., Wu D., Li W., Zheng X., Li W, & Shan A. (2017). High Specific Selectivity and Membrane-Active Mechanism of Synthetic Cationic Hybrid Antimicrobial Peptides Based on the Peptide FV7. International Journal of Molecular Sciences, 18(2), 339.

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Antimicrobial Activity Evaluation Protocol

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The bacterial strains Enterococcus faecalis ATCC 29212, Staphylococcus epidermidis ATCC 12228, Staphylococcus aureus ATCC 29213, Salmonella typhimurium C7731, Escherichia coli ATCC 25922 and Salmonella Pullorum C7913 were obtained from the College of Veterinary Medicine, Northeast Agricultural University (Harbin, China). The human red blood cells (hRBCs) were obtained from the Northeast Agricultural University Hospital.
Phosphate-buffered saline (PBS) solution was purchased from Kermel (China). Mueller Hinton Broth (MHB) powder was purchased from AoBoX (China) and used to prepare the microbial broths according to the manufacturer’s instructions. 3,3′-dipropylthiadicarbocyanine (diSC₃-5), dimethyl sulfoxide (DMSO), ethanol (analytical grade, 99%), tertiary butanol (analytical grade, 99%), acetone (analytical grade, 99%), glutaraldehyde (synthetic grade, 50% in H₂O) and propidium iodide (PI) were all ordered from Sigma-Aldrich (China).

Xu L., Shao C., Li G., Shan A., Chou S., Wang J., Ma Q, & Dong N. (2020). Conversion of Broad-Spectrum Antimicrobial Peptides into Species-Specific Antimicrobials Capable of Precisely Targeting Pathogenic Bacteria. Scientific Reports, 10, 944.

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Lipid Peroxidation Measurement in LX2 Cells

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Measurement of lipid peroxidation was carried out by a previously described method [13 (link)]. Briefly, 1X10⁵ cells were plated in six well plates prior to treatment with INH. Following overnight culture, LX2 cells were treated with INH for 24 to 72 hours. After treatment, the cells were trypsinized and suspended in PBS. The suspended cells were subjected to sonication followed by centrifugation of the cell lysate at 14000 rpm for 20 minutes. Protein was determined from the supernatant using Bradford method. To 100 μl of cell supernatant, 200 μl of 10% TCA and 400 μl of 0.67% thiobarbituric acid (TBA; T5500; Sigma Aldrich, St. Louis, MO) were added in a boiling water bath for 45 min. After being cooled in an ice bath, equal volume of tertiary butanol (19460; Sigma Aldrich, St. Louis, MO) was added to the samples followed by centrifugation at 10000 rpm for 10 min. The absorbance of the resulting supernatant was read at 532 nm to determine formation of TBA reactive components.

Santra S., Bishnu D., Dhali G.K., Santra A, & Chowdhury A. (2020). Expression of type I collagen in response to Isoniazid exposure is indirect and is facilitated by collateral induction of cytochrome P450 2E1: An in-vitro study. PLoS ONE, 15(7), e0236992.

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