Inveon small animal pet ct scanner

Two groups of mice, ApoE-KO (n = 15) and control (n = 6), were used for in vivo and ex vivo imaging studies. Mice were kept fully sedated with 1.5–2% isoflurane during injections and PET/CT imaging. Images were acquired using the Inveon small animal PET/CT scanner (Siemens, Knoxville, TN, USA) 1 h after i.v. injection of ⁶⁸Ga-NOTA-anti-MMR Nb (8–10 MBq, 3–4 μg). Briefly, CT anatomic images were acquired (80 kV, 500 μA) with a pixel size of 0.1 mm. After CT imaging, static PET images were acquired with an acquisition time of 20 min. Images were reconstructed as single frames using Siemens Inveon software, employing a 3-dimensional ordered subsets expectation maximum algorithm (OSEM3D) without scatter and attenuation correction. A group of ApoE-KO mice (n = 4), referred to as the ApoE-KO blocked group, was co-injected with blocking dose (100-fold excess) of non-labelled NOTA-anti-MMR Nb. For quantification of vascular uptake, circular regions of interest (ROIs) were placed on axial PET/CT images of the thoracic and abdominal aortas and signal intensities were recorded as kBq/cc. ROIs were identified by the same person with their centres at the point of local maximum ⁶⁸Ga-NOTA-anti-MMR Nb uptake.

Prior to imaging, mice were injected intravenously (tail vein) with 100 μCi of ⁸⁹Zr-Df-Bz-NCS-Ab. At three and seven days post-injection mice were anaesthetized with 1%-2% isoflurane and imaged with an Inveon small animal PET/CT scanner (Siemens Medical Solutions). Static images were collected for 20 min and reconstructed with the Maximum A Posteriory (MAP) probability algorithm [27 (link)] followed by co-registration with the Inveon Research Workplace 4.0 (IRW) image display software (Siemens Medical Solutions, Knoxville, TN). Regions of interest (ROI) were selected from PET images using CT anatomical guidelines and the activity determined with IRW software. Standard uptake values (SUV) were determined as nCi/cc x animal weight/injected dose. To block the binding of ⁸⁹Zr labeled antibody, 40 mg/kg of unlabeled antibody was injected intravenously (tail vein) 10 minutes prior to the labeled antibody.

Two groups of mice, ApoE-KO (n = 12) and control (n = 6), were used for in vivo and ex vivo imaging. Mice were kept sedated with 1.5-2% isoflurane during injections and PET/CT scans. Static images were acquired 48 h after i.v. injections of ⁸⁹Zr-DFO-Gal3-F(ab')2 mAb (2.2 ± 0.2 MBq, 8-9 µg) using the Inveon small animal PET/CT scanner (Siemens, Knoxville, TN, USA) with an acquisition time of 20 min. Images were reconstructed using Siemens Inveon software, which employs a 3-dimensional ordered subsets expectation maximum (OSEM3D) algorithm without attenuation and scatter correction. To confirm tracer uptake specificity, a group of ApoE-KO mice (n = 3) was co-injected with a 100-fold excess of unlabeled full-length rat anti Gal3 mAb, and referred as ApoE-KO blocked.