Anti lkb1

For IHC staining, paraffin sections of murine tissues were deparaffinized with xylene and rehydrated with ethanol at decreasing concentrations. Then, samples were incubated with the indicated antibodies overnight at 4 °C followed by incubating with the correspondent secondary antibodies for 1 h at room temperature. Nuclei were stained with hematoxylin and images were captured using an inverted microscope (Olympus). The following antibodies were used in IHC: anti‐ACC1 (CST, 3676), anti‐p‐ACC1 (CST, 3661), anti‐AMPK (CST, 5832), anti‐p‐AMPK (CST, 2535), anti‐LKB1 (Immunoway, YT2573), anti‐Vim (Bioss, bs‐8533R), anti‐Ecad (Bioss, bs‐10009R), anti‐HNRNPD (Thermo, PA5‐99469). The average optical density (AOD) is calculated by ImageJ to indicate the IHC signal.

For western blots, samples were separated on SDS‐PAGE gels and then transferred to PVDF membranes (Millipore). Membranes were processed according to the ECL western blotting protocol (GE Healthcare). The grayscale statistics of western blotting were performed by ImageJ. The following antibodies were used in western blots: anti‐AMPK (CST, 5832), anti‐p‐AMPK (CST, 2535), anti‐ACC1 (CST, 3676), anti‐p‐ACC1 (CST, 3661), anti‐RAPTOR (CST, 2280), anti‐p‐RAPTOR (CST, 89146), anti‐VEGFA (Proteintech, 9003‐1‐AP), anti‐TGF‐β (Immunoway, YT4632), anti‐MFF (Proteintech, 17090‐1‐AP), anti‐p‐MFF (Immunoway, YP1403), anti‐p‐ULK1 (CST, 5869), anti‐ULK1 (CST, 8054), anti‐LKB1 (Immunoway, YT2573), anti‐HNRNPD (Thermo, PA5‐99469), anti‐AGO2 (Sigma, SAB4200085), anti‐eIF4G1 (CST, 8701), anti‐eIF4E (Abcam, ab33768), anti‐c‐MYC (CST, 9402), and anti‐ACTB (TransGen, HC201). Antibody validation is provided on the manufacturers’ websites.