Nadph

Cisplatin (DDP) was purchased from Aladdin (Shanghai, China). NADPH and 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) were obtained from Biosharp (Guangzhou, China). Aurothioglucose (ATG) was purchased from WAKO (Tokyo, Japan). Glutathione reductase (GR) and iodoacetamide (IAM) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RIPA lysis buffer was obtained from Beyotime Biotechnology (Shanghai, China). Selenium-deficient (SD, 0.01 mg/kg), selenium-normal (SN, 0.21 mg/kg), and selenium-enriched (SE, 1.01 mg/kg) diets were manufactured by Medicience (Nanning, China). All other reagents were obtained from the Sigma-Aldrich Chemical Co. (St. Louis, MO, USA) or Fisher Scientific Ltd. (Waltham, MA, USA). The pure recombinant wild-type rat TrxR1 protein, and anti-Trx1 and anti-TrxR1 antibodies were obtained from IMCO Corporation, Stockholm, Sweden (www.imcocorp.se accessed on 1 January 2020). Other antibodies were obtained from Proteintech (Wuhan, China). C57BL/6j mice were provided by Ensiweier (Chongqing, China).

Hon (purity ≥ 99%) was obtained from TCAS Chem Tech Co., Ltd. (Wuhan, China); Strepotozotocin, hydroxybupropion and 12-hydroxy Lauric acid were purchased from Sigma Chem Co., Ltd. (St. Louis, MO, USA). Bupropion and 6β-hydroxy testosterone were purchased from Cayman Chem Co., (Ann Arbor, MI, USA). Phenacetinwas obtained from Alfa Aesar Chem Co., Ltd. (Tianjin, China); Chlorzoxazone was purchased from National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Tolbutamide and testosterone were purchased from Dr. Ehrenstorfer GmbH, (Augsburg, Germany). Lauric acid was purchased from Nu-Chek Prep, Inc. (Los Angeles, CA, USA). Acetaminophen was purchased from TCI (Tokyo, Japan). 6-hydroxychlorzoxazoneand 4-hydroxyTolbutamidewere purchased from TRC (Toronto, ON, Canada). NADPH was purchased from Biosharp (Hefei, China). HPLC-grade methanol and acetonitrile were purchased from Tedia Co., Inc. (Fairfield, OH, USA). RT-PCR Kit and Trans Start Green qPCR Super Mix were purchased from Toyobo Co., Ltd. (Osaka, Japan). Real-time fluorescent quantitative PCR primers were purchased from Sunny Biotech. Co., Ltd. (Shanghai, China). All other chemicals and reagents used in the study were of analytical grade.