Log In Sign up
The largest database of trusted experimental protocols

Criteriontris glycine extended gels

Manufactured by Bio-Rad
Visit Supplier

The CriterionTris-Glycine eXtended (TGX) gels are pre-cast polyacrylamide gels used for protein electrophoresis. They are designed to provide high-resolution separation of proteins across a wide molecular weight range.

Automatically generated - may contain errors

Lab products found in correlation

Azure c600, by Azure Biosystems (4 mentions) Laemmli sample buffer, by Bio-Rad (4 mentions) Cell lysis buffer, by Cell Signaling Technology (3 mentions) Trans blot turbo system, by Bio-Rad (3 mentions) Ab76024, by Abcam (2 mentions) Ab109235, by Cell Signaling Technology (2 mentions) Vinculin, by Merck Group (2 mentions) Antibodygoat anti rabbit or goat anti mouse igg hrp, by Thermo Fisher Scientific (2 mentions) Image studio lite software version 5, by LI COR (2 mentions) 2 mercaptoethanol, by Merck Group (2 mentions) Cell lysis buffer 6, by R&D Systems (1 mentions) β actin a1978, by Merck Group (1 mentions) Vinculin v9131, by Merck Group (1 mentions)

Close spelling variants of this product

Glycine

4 protocols using criteriontris glycine extended gels

1

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
1*106 cells were lysed (cell lysis buffer, Cell signaling
technology) and denatured in 1x Laemmli sample buffer (Bio-rad) containing
2-mercaptoethanol (Sigma Aldrich). Proteins were separated on Criterion
Tris-Glycine eXtended gels (Bio-rad), transferred to PVDF membranes using the
Trans-Blot Turbo system (Bio-rad), incubated with primary antibodies targeting
Catalase (Cell Signaling #14097 & Abcam #ab76024), xanthine oxidase
(Abcam #ab109235), Bcl-2 (Cell Signaling #2870), and with secondary antibody
Goat Anti-Rabbit or Goat Anti-Mouse IgG-HRP (Thermo Fischer). Proteins were
visualized using chemiluminescence on an Azure C600 (Azure Biosystems).
Quantification was performed using LI-COR Image Studio Lite software version
5.2. Vinculin (Sigma Aldrich), tubulin or beta actin (Sigma Aldrich) was used to
normalize for protein input.
Kampen K.R., Sulima S.O., Verbelen B., Girardi T., Vereecke S., Rinaldi G., Verbeeck J., de Beeck J.O., Uyttebroeck A., Meijerink J.P., Moorman A.V., Harrison C.J., Spincemaille P., Cools J., Cassiman D., Fendt S.M., Vermeersch P, & De Keersmaecker K. (2018). The ribosomal RPL10 R98S mutation drives IRES-dependent BCL-2 translation in T-ALL. Leukemia, 33(2), 319-332.
+ Open protocol
+ Expand
2

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed (cell lysis buffer 6, R&D Systems) and denatured in 1× Laemmli sample buffer (Bio-Rad) containing 2-mercaptoethanol (Sigma-Aldrich). Proteins were separated on Criterion Tris-Glycine eXtended gels (Bio-rad), transferred to PVDF membranes, and incubated with primary and secondary antibodies (Supplementary Table 3). Proteins were visualised using chemiluminescence on an Azure C600 (Azure Biosystems). Quantification was performed using LI-COR Image Studio Lite software version 5.2. Vinculin and β-actin were used to normalise for protein input. Immunoblots were performed on samples from at least three biological repeat experiments.
Heylen E., Verstraete P., Van Aerschot L., Geeraerts S.L., Venken T., Timcheva K., Nittner D., Verbeeck J., Royaert J., Gijbels M., Uyttebroeck A., Segers H., Lambrechts D., Cools J., De Keersmaecker K, & Kampen K.R. (2023). Transcription factor NKX2–1 drives serine and glycine synthesis addiction in cancer. British Journal of Cancer, 128(10), 1862-1878.
+ Open protocol
+ Expand
3

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
1*106 cells were lysed (cell lysis buffer, Cell signaling
technology) and denatured in 1x Laemmli sample buffer (Bio-rad) containing
2-mercaptoethanol (Sigma Aldrich). Proteins were separated on Criterion
Tris-Glycine eXtended gels (Bio-rad), transferred to PVDF membranes using the
Trans-Blot Turbo system (Bio-rad), incubated with primary antibodies targeting
Catalase (Cell Signaling #14097 & Abcam #ab76024), xanthine oxidase
(Abcam #ab109235), Bcl-2 (Cell Signaling #2870), and with secondary antibody
Goat Anti-Rabbit or Goat Anti-Mouse IgG-HRP (Thermo Fischer). Proteins were
visualized using chemiluminescence on an Azure C600 (Azure Biosystems).
Quantification was performed using LI-COR Image Studio Lite software version
5.2. Vinculin (Sigma Aldrich), tubulin or beta actin (Sigma Aldrich) was used to
normalize for protein input.
Kampen K.R., Sulima S.O., Verbelen B., Girardi T., Vereecke S., Rinaldi G., Verbeeck J., de Beeck J.O., Uyttebroeck A., Meijerink J.P., Moorman A.V., Harrison C.J., Spincemaille P., Cools J., Cassiman D., Fendt S.M., Vermeersch P, & De Keersmaecker K. (2018). The ribosomal RPL10 R98S mutation drives IRES-dependent BCL-2 translation in T-ALL. Leukemia, 33(2), 319-332.
+ Open protocol
+ Expand
4

Western Blot Analysis of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed (cell lysis buffer, Cell Signaling Technology) and denatured in 1x Laemmli sample buffer (Bio-rad) containing 2-mercaptoethanol (Sigma Aldrich). Proteins were separated on Criterion Tris-Glycine eXtended gels (Bio-rad), transferred to PVDF membranes using a Trans-Blot Turbo system (Bio-rad), incubated with primary antibodies targeting PSPH (Bioconnect, 14513-1-AP, 1:1000), phospho-CDK2 (4539, 1:1000) or Helios (42427, 1:1000) (Cell Signaling Technology), and with secondary Goat Anti-Rabbit (31462, 1:2000) or Goat Anti-Mouse (31432, 1:2000) IgG-HRP antibody (ThermoFischer Scientific) or near infrared anti-Mouse (5470, 1:1000) or anti-Rabbit (5151, 1:1000) antibodies (Cell Signaling Technology). Proteins were visualized using fluorescent secondary antibodies or chemiluminescence on an Azure C600 (Azure Biosystems). Quantification was performed using LI-COR Image Studio Lite software version 5.2. Vinculin (V9131, 1:4000) or β-actin (A 1978, 1:4000) (Sigma Aldrich) were used to normalize for protein input. Immunoblots were repeated at least three times and representative blots are shown in the figures and in full size in Supplementary Fig. 18.
Kampen K.R., Fancello L., Girardi T., Rinaldi G., Planque M., Sulima S.O., Loayza-Puch F., Verbelen B., Vereecke S., Verbeeck J., Op de Beeck J., Royaert J., Vermeersch P., Cassiman D., Cools J., Agami R., Fiers M., Fendt S.M, & De Keersmaecker K. (2019). Translatome analysis reveals altered serine and glycine metabolism in T-cell acute lymphoblastic leukemia cells. Nature Communications, 10, 2542.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!