Pdgf 220 bb

Manufactured by R&D Systems

Sourced in United States

PDGF (220-BB) is a recombinant human Platelet-Derived Growth Factor-BB homodimer. It is a 30 kDa disulfide-linked homodimer that belongs to the PDGF family of growth factors. PDGF-BB is involved in the regulation of cell growth and division.

Automatically generated - may contain errors

Lab products found in correlation

Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (2 mentions) Mouse anti gapdh sc 47724, by Santa Cruz Biotechnology (1 mentions) Silymarin s0292, by Merck Group (1 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Gemcitabine hydrochloride, by Fujifilm (1 mentions) Trypsin solution, by Thermo Fisher Scientific (1 mentions) Biocoat matrigel invasion chamber, by BD (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) High capacity rna to cdna kit, by Thermo Fisher Scientific (1 mentions) Collagen 4, by Merck Group (1 mentions) Supersignal west pico and femto chemiluminescent substrates, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Fugene hd transfection reagent, by Promega (1 mentions) Fibronectin, by BD (1 mentions)

Spelling variants of this product

PDGF-BB (171 citations)

2 protocols using pdgf 220 bb

Evaluating PQQ and NAC Effects on TGF-β1 and PDGF Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human TGF-β1 (240-B-002) and PDGF (220-BB) were obtained from R&D system. Rabbit anti-phospho-JNK (#9251),-JNK (#9252),-phospho-p38 (#4511),-p38 (#9212),-phospho-Smad2 (#3108),-phospho-Smad3 (#9520),-Smad2/3 (#5678),-phospho-AKT (#2965),-AKT (#4691) and Rabbit anti-cleaved caspase-3 (#9664) were purchased from Cell Signaling Technology. Rabbit anti-phospho-ERK1/2 (sc-101760),-ERK1/2 (sc-292838), mouse anti-GAPDH (sc-47724), and goat anti-Col1A1 (sc-8784) antibodies were obtained from Santa Cruz Biotechnology. Rat anti-F4/80 (MCA497RT) antibody was obtained from Serotec. Sheep anti-albumin (ab8940) and mouse anti-CD68 (ab955) antibodies were purchased from Abcam. Pyrroloquinoline-quinone (PQQ) (D7783), mouse anti-α-SMA antibody (A2547), N-acetylcysteine (NAC) (A7250) and silymarin (S0292) were purchased from Sigma Aldrich. For in vitro studies, PQQ was used at increasing doses of 0.3 μg/ml, 3 μg/ml and 30 μg/ml if not indicated. NAC served as a positive control and was used at the dose of 800 μg/ml.

Jia D., Duan F., Peng P., Sun L., Ruan Y, & Gu J. (2015). Pyrroloquinoline-Quinone Suppresses Liver Fibrogenesis in Mice. PLoS ONE, 10(3), e0121939.

+ Open protocol

+ Expand

Detailed Reagents and Materials Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following items were purchased: fetal bovine serum (FBS), D MEM, RPMI, MEM and trypsin solution from Gibco (Life Technologies); RNeasy Mini kit from Qiagen (Hilden, Germany); High Capacity RNA-to-cDNA kit from Applied Biosystems (Darmstadt, Germany); NE-PER Nuclear and Cytoplasmic Extraction reagents and SuperSignal West Pico and Femto chemiluminescent substrates from Thermo Fisher Scientific (Rockford, IL, USA); FuGENE HD Transfection reagent from Promega (Madison, WI, USA); G418 (Geneticin) from Roche Diagnostics Deutschland GmbH (Mannheim, Germany); laminin, collagen from human placenta type IV [(collagen IV) from Sigma-Aldrich]; Cell Counting kit-8 from Dojindo Laboratories (Kumamoto, Japan); BioCoat Matrigel Invasion Chamber (8-µm pore size) and fibronectin from BD Biosciences (Franklin Lakes, NJ, USA); gemcitabine hydrochloride from Wako Pure Chemical Industries (Osaka, Japan); Fluorescent Mounting Medium from Dako Japan (Tokyo, Japan); Histofine Simple Stain Max PO (M) or (R) kit from Nichirei Biosciences, Inc., (Tokyo, Japan); and recombinant human epidermal growth factor (EGF, 236-EG) and recombinant human platelet-derived growth factor (PDGF, 220-BB) from R&D Systems (Minneapolis, MN, USA).

SUMIYOSHI H., MATSUSHITA A., NAKAMURA Y., MATSUDA Y., ISHIWATA T., NAITO Z, & UCHIDA E. (2016). Suppression of STAT5b in pancreatic cancer cells leads to attenuated gemcitabine chemoresistance, adhesion and invasion. Oncology Reports, 35(6), 3216-3226.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!